PMC:8950415 / 9187-10041 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/8950415","sourcedb":"PMC","sourceid":"8950415","source_url":"https://www.ncbi.nlm.nih.gov/pmc/8950415","text":"SLAG operation: To avoid damaging the perforated disc elements, the pressure was gradually increased by increasing the air flow applied in increments for each run. Similarly, the volume of virus inoculum applied during operation was altered to produce visible, vigorous bursting bubbles without excessive foaming, by increasing and decreasing the speed of the peristaltic pump used to aliquot the liquid. It was found, especially for the 90 mm SLAG, that pre-wetting the perforated 90 mm disc moments before applying compressed air to the system, achieved maximal levels of bubble bursting (sparging). Due to limitations on time, the air pressure and inoculum volume were not fully optimised for the 1 inch SLAG, however the ranges applied to both disc sizes appear in Table 1. All other nebulisers were used according to the manufacturer’s instructions.","tracks":[]}