PMC:8950092 / 9756-10153 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/8950092","sourcedb":"PMC","sourceid":"8950092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/8950092","text":"Phage Isolation and Propagation via Plate Lysate\nAfter incubating the LB agar plate with the bacteria to form plaques at 37 °C, the plate was lysed by pouring 5 mL of SM buffer on top of the plate and shaking, gently, for 15 min. The phage suspension in the buffer was collected and centrifuged at 4000× g for 5 min to remove cell debris. The phage lysate was filter sterilized and stored at 4 °C.","divisions":[{"label":"title","span":{"begin":0,"end":48}}],"tracks":[]}