PMC:8950092 / 22324-24603 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/8950092","sourcedb":"PMC","sourceid":"8950092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/8950092","text":"Tests were conducted at 60% and 80% relative humidity at room temperature. Figure 6 shows the total gene copy numbers (GCN) of BCoV per milliliter (mL) in the 60% and 80% relative humidity testing of the stock and nebulized solution samples quantitated by real-time qPCR testing. The total GCN/mL in the stock and nebulized solution were 9.720 × 108 and 6.979 × 108 for 60% relative humidity and 1.034 × 109 and 8.162 × 108 for 80% relative humidity, respectively. Figure 7 and Figure 8 show the total BCoV gene copy numbers (GCN) per liter air (L Air) for the aerosol samples collected by the two wetted wall cyclones and the MD8 sampler, and the total BCoV gene copy numbers per cm2 for the chamber and surface swab samples in the 60% and 80% relative humidity testing. The total GCN/L Air for the WWC, 3-D WWC, and MD8 air samplers were 6.432 × 105, 1.645 × 106, and 2.731 × 107 for 60% relative humidity and 1.841 × 105, 1.239 × 106, and 3.618 × 106 for 80% relative humidity, respectively. The total GCN/cm2 for the chamber, wood, metal, and plastic surface swab samples were 4.547 × 106, 6.377 × 106, 4.444 × 106, and 4.534 × 106 for 60% relative humidity and 3.590 × 106, 1.597 × 107, 8.162 × 106, and 4.329 × 106 for 80% relative humidity, respectively. Based on statistical analysis performed, only the stock solution sample had significant difference between replicates with a p-value of 0.0448. Based on the results from the ANOVA test, all samples had significant difference between replicates with each p-value being below 0.05. Higher counts of BCoV were found in the 60% relative humidity testing samples compared to the 80% relative humidity testing samples. The WWC and MD8 Sartorius aerosol samples were the only sets to have significantly different total BcoV gene copy numbers per sample collected. The p-value attained from the ANOVA test between the 60% and 80% relative humidity tests for the WWC and MD8 samplers were 0.0147 and 9.30 × 10−5. The chamber and wood swab samples had p-values of 0.0469 and 0.0134, showing significant difference between the samples at 60% and 80% relative humidity. At 80% relative humidity there was significant difference between the chamber, plastic, metal, and wood samples as the ANOVA test returned a p-value of 0.0007.","tracks":[]}