PMC:8950092 / 18944-19858 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/8950092","sourcedb":"PMC","sourceid":"8950092","source_url":"https://www.ncbi.nlm.nih.gov/pmc/8950092","text":"2.3.2. Sample Assessment\nFor preliminary analysis of viable virus collection, each of the 12 filters collected in each test was placed in 2 mL of PBS. Aliquots of 4 mL of soft agar medium were added to glass tubes with 100 µg/mL Ampicillin. Aliquots of 100 μL of each collected sample in 10x dilution and 100 μL of the fresh mid-log phase S. enterica LT2 RD1 host were added to the medium and poured over a plate. A 10−6 dilution of either the nebulized liquid or the stock solution 100 μL was added to the soft agar medium with 100 μL of the Salmonella host and poured over an LB plate. The same process was performed with the resuspended exhaust filter. The plates were placed in the incubator overnight at 37 °C and the plaque forming units (PFUs) were counted the next morning. Total PRD1 phage numbers were quantitated by qPCR using species specific primers and ABI qPCR chemistry as described in Section 2.2.","divisions":[{"label":"title","span":{"begin":0,"end":24}}],"tracks":[]}