Id |
Subject |
Object |
Predicate |
Lexical cue |
T2105 |
0-16 |
NN |
denotes |
Characterization |
T2106 |
17-19 |
IN |
denotes |
of |
T2107 |
20-23 |
DT |
denotes |
the |
T2108 |
50-57 |
NN |
denotes |
variant |
T2109 |
24-30 |
NN |
denotes |
Mcoln1 |
T2110 |
31-42 |
JJ |
denotes |
alternative |
T2111 |
43-49 |
NN |
denotes |
splice |
T2112 |
57-237 |
sentence |
denotes |
In order to determine the coding sequence for the larger transcript, we searched the mouse EST database using each intron as well as the genomic sequence flanking the Mcoln1 gene. |
T2113 |
58-60 |
IN |
denotes |
In |
T2114 |
130-138 |
VBD |
denotes |
searched |
T2115 |
61-66 |
NN |
denotes |
order |
T2116 |
67-69 |
TO |
denotes |
to |
T2117 |
70-79 |
VB |
denotes |
determine |
T2118 |
80-83 |
DT |
denotes |
the |
T2119 |
91-99 |
NN |
denotes |
sequence |
T2120 |
84-90 |
VBG |
denotes |
coding |
T2121 |
100-103 |
IN |
denotes |
for |
T2122 |
104-107 |
DT |
denotes |
the |
T2123 |
115-125 |
NN |
denotes |
transcript |
T2124 |
108-114 |
JJR |
denotes |
larger |
T2125 |
125-127 |
, |
denotes |
, |
T2126 |
127-129 |
PRP |
denotes |
we |
T2127 |
139-142 |
DT |
denotes |
the |
T2128 |
153-161 |
NN |
denotes |
database |
T2129 |
143-148 |
NN |
denotes |
mouse |
T2130 |
149-152 |
NN |
denotes |
EST |
T2131 |
162-167 |
VBG |
denotes |
using |
T2132 |
168-172 |
DT |
denotes |
each |
T2133 |
173-179 |
NN |
denotes |
intron |
T2134 |
180-182 |
RB |
denotes |
as |
T2135 |
188-190 |
IN |
denotes |
as |
T2136 |
183-187 |
RB |
denotes |
well |
T2137 |
191-194 |
DT |
denotes |
the |
T2138 |
203-211 |
NN |
denotes |
sequence |
T2139 |
195-202 |
JJ |
denotes |
genomic |
T2140 |
212-220 |
VBG |
denotes |
flanking |
T2141 |
221-224 |
DT |
denotes |
the |
T2142 |
232-236 |
NN |
denotes |
gene |
T2143 |
225-231 |
NN |
denotes |
Mcoln1 |
T2144 |
236-237 |
. |
denotes |
. |
T2145 |
237-378 |
sentence |
denotes |
Two ESTs were identified that contained sequence from intron 12 (GB No. AI430291 and AA874645), and the corresponding clones were sequenced. |
T2146 |
238-241 |
CD |
denotes |
Two |
T2147 |
242-246 |
NNS |
denotes |
ESTs |
T2148 |
252-262 |
VBN |
denotes |
identified |
T2149 |
247-251 |
VBD |
denotes |
were |
T2150 |
263-267 |
WDT |
denotes |
that |
T2151 |
268-277 |
VBD |
denotes |
contained |
T2152 |
278-286 |
NN |
denotes |
sequence |
T2153 |
287-291 |
IN |
denotes |
from |
T2154 |
292-298 |
NN |
denotes |
intron |
T2155 |
299-301 |
CD |
denotes |
12 |
T2156 |
302-303 |
-LRB- |
denotes |
( |
T2157 |
310-318 |
NN |
denotes |
AI430291 |
T2158 |
303-305 |
NN |
denotes |
GB |
T2159 |
306-309 |
NN |
denotes |
No. |
T2160 |
319-322 |
CC |
denotes |
and |
T2161 |
323-331 |
NN |
denotes |
AA874645 |
T2162 |
331-332 |
-RRB- |
denotes |
) |
T2163 |
332-334 |
, |
denotes |
, |
T2164 |
334-337 |
CC |
denotes |
and |
T2165 |
338-341 |
DT |
denotes |
the |
T2166 |
356-362 |
NNS |
denotes |
clones |
T2167 |
342-355 |
JJ |
denotes |
corresponding |
T2168 |
368-377 |
VBN |
denotes |
sequenced |
T2169 |
363-367 |
VBD |
denotes |
were |
T2170 |
377-378 |
. |
denotes |
. |
T2171 |
378-533 |
sentence |
denotes |
Clone 408619 (ESTs: GB No. AI430291, AI429558) begins approximately 1.1 kb before exon 13 and continues through the exon and splices correctly to exon 14. |
T2172 |
379-384 |
NN |
denotes |
Clone |
T2173 |
426-432 |
VBZ |
denotes |
begins |
T2174 |
385-391 |
CD |
denotes |
408619 |
T2175 |
392-393 |
-LRB- |
denotes |
( |
T2176 |
416-424 |
NN |
denotes |
AI429558 |
T2177 |
393-397 |
NNS |
denotes |
ESTs |
T2178 |
397-399 |
: |
denotes |
: |
T2179 |
399-401 |
NN |
denotes |
GB |
T2180 |
402-405 |
NN |
denotes |
No. |
T2181 |
406-414 |
NN |
denotes |
AI430291 |
T2182 |
414-416 |
, |
denotes |
, |
T2183 |
424-425 |
-RRB- |
denotes |
) |
T2184 |
433-446 |
RB |
denotes |
approximately |
T2185 |
447-450 |
CD |
denotes |
1.1 |
T2186 |
451-453 |
NNS |
denotes |
kb |
T2187 |
454-460 |
IN |
denotes |
before |
T2188 |
461-465 |
NN |
denotes |
exon |
T2189 |
466-468 |
CD |
denotes |
13 |
T2190 |
469-472 |
CC |
denotes |
and |
T2191 |
473-482 |
VBZ |
denotes |
continues |
T2192 |
483-490 |
IN |
denotes |
through |
T2193 |
491-494 |
DT |
denotes |
the |
T2194 |
495-499 |
NN |
denotes |
exon |
T2195 |
500-503 |
CC |
denotes |
and |
T2196 |
504-511 |
VBZ |
denotes |
splices |
T2197 |
512-521 |
RB |
denotes |
correctly |
T2198 |
522-524 |
IN |
denotes |
to |
T2199 |
525-529 |
NN |
denotes |
exon |
T2200 |
530-532 |
CD |
denotes |
14 |
T2201 |
532-533 |
. |
denotes |
. |
T2202 |
533-637 |
sentence |
denotes |
Clone 1281641 (EST:GB No. AA874645) begins 175 bp before exon 13 and also splices correctly to exon 14. |
T2203 |
534-539 |
NN |
denotes |
Clone |
T2204 |
570-576 |
VBZ |
denotes |
begins |
T2205 |
540-547 |
CD |
denotes |
1281641 |
T2206 |
548-549 |
-LRB- |
denotes |
( |
T2207 |
560-568 |
NN |
denotes |
AA874645 |
T2208 |
549-552 |
NN |
denotes |
EST |
T2209 |
552-553 |
: |
denotes |
: |
T2210 |
553-555 |
NN |
denotes |
GB |
T2211 |
556-559 |
NN |
denotes |
No. |
T2212 |
568-569 |
-RRB- |
denotes |
) |
T2213 |
577-580 |
CD |
denotes |
175 |
T2214 |
581-583 |
NNS |
denotes |
bp |
T2215 |
584-590 |
IN |
denotes |
before |
T2216 |
591-595 |
NN |
denotes |
exon |
T2217 |
596-598 |
CD |
denotes |
13 |
T2218 |
599-602 |
CC |
denotes |
and |
T2219 |
603-607 |
RB |
denotes |
also |
T2220 |
608-615 |
VBZ |
denotes |
splices |
T2221 |
616-625 |
RB |
denotes |
correctly |
T2222 |
626-628 |
IN |
denotes |
to |
T2223 |
629-633 |
NN |
denotes |
exon |
T2224 |
634-636 |
CD |
denotes |
14 |
T2225 |
636-637 |
. |
denotes |
. |
T2226 |
637-826 |
sentence |
denotes |
A mouse multiple tissue Northern was hybridized using a probe generated from the putative intron sequence in clone 408619 (Probe 2, Fig. 1B), which detected only the 4.4 kb band (Fig. 3C). |
T2227 |
638-639 |
DT |
denotes |
A |
T2228 |
662-670 |
NNP |
denotes |
Northern |
T2229 |
640-645 |
NN |
denotes |
mouse |
T2230 |
646-654 |
JJ |
denotes |
multiple |
T2231 |
655-661 |
NN |
denotes |
tissue |
T2232 |
675-685 |
VBN |
denotes |
hybridized |
T2233 |
671-674 |
VBD |
denotes |
was |
T2234 |
686-691 |
VBG |
denotes |
using |
T2235 |
692-693 |
DT |
denotes |
a |
T2236 |
694-699 |
NN |
denotes |
probe |
T2237 |
700-709 |
VBN |
denotes |
generated |
T2238 |
710-714 |
IN |
denotes |
from |
T2239 |
715-718 |
DT |
denotes |
the |
T2240 |
735-743 |
NN |
denotes |
sequence |
T2241 |
719-727 |
JJ |
denotes |
putative |
T2242 |
728-734 |
NN |
denotes |
intron |
T2243 |
744-746 |
IN |
denotes |
in |
T2244 |
747-752 |
NN |
denotes |
clone |
T2245 |
753-759 |
CD |
denotes |
408619 |
T2246 |
760-761 |
-LRB- |
denotes |
( |
T2247 |
775-777 |
NN |
denotes |
1B |
T2248 |
761-766 |
NN |
denotes |
Probe |
T2249 |
767-768 |
CD |
denotes |
2 |
T2250 |
768-770 |
, |
denotes |
, |
T2251 |
770-774 |
NNP |
denotes |
Fig. |
T2252 |
777-778 |
-RRB- |
denotes |
) |
T2253 |
778-780 |
, |
denotes |
, |
T2254 |
780-785 |
WDT |
denotes |
which |
T2255 |
786-794 |
VBD |
denotes |
detected |
T2256 |
795-799 |
RB |
denotes |
only |
T2257 |
811-815 |
NN |
denotes |
band |
T2258 |
800-803 |
DT |
denotes |
the |
T2259 |
804-807 |
CD |
denotes |
4.4 |
T2260 |
808-810 |
NN |
denotes |
kb |
T2261 |
816-817 |
-LRB- |
denotes |
( |
T2262 |
822-824 |
NN |
denotes |
3C |
T2263 |
817-821 |
NN |
denotes |
Fig. |
T2264 |
824-825 |
-RRB- |
denotes |
) |
T2265 |
825-826 |
. |
denotes |
. |
T2266 |
826-1048 |
sentence |
denotes |
In order to determine the sequence of the entire transcript, RT-PCR using primers in exons 10 and 11 paired with a primer in intron 12 was performed using BALB/c mouse brain total RNA and the resulting products sequenced. |
T2267 |
827-829 |
IN |
denotes |
In |
T2268 |
966-975 |
VBN |
denotes |
performed |
T2269 |
830-835 |
NN |
denotes |
order |
T2270 |
836-838 |
TO |
denotes |
to |
T2271 |
839-848 |
VB |
denotes |
determine |
T2272 |
849-852 |
DT |
denotes |
the |
T2273 |
853-861 |
NN |
denotes |
sequence |
T2274 |
862-864 |
IN |
denotes |
of |
T2275 |
865-868 |
DT |
denotes |
the |
T2276 |
876-886 |
NN |
denotes |
transcript |
T2277 |
869-875 |
JJ |
denotes |
entire |
T2278 |
886-888 |
, |
denotes |
, |
T2279 |
888-890 |
NN |
denotes |
RT |
T2280 |
891-894 |
NN |
denotes |
PCR |
T2281 |
890-891 |
HYPH |
denotes |
- |
T2282 |
895-900 |
VBG |
denotes |
using |
T2283 |
901-908 |
NNS |
denotes |
primers |
T2284 |
909-911 |
IN |
denotes |
in |
T2285 |
912-917 |
NNS |
denotes |
exons |
T2286 |
918-920 |
CD |
denotes |
10 |
T2287 |
921-924 |
CC |
denotes |
and |
T2288 |
925-927 |
CD |
denotes |
11 |
T2289 |
928-934 |
VBN |
denotes |
paired |
T2290 |
935-939 |
IN |
denotes |
with |
T2291 |
940-941 |
DT |
denotes |
a |
T2292 |
942-948 |
NN |
denotes |
primer |
T2293 |
949-951 |
IN |
denotes |
in |
T2294 |
952-958 |
NN |
denotes |
intron |
T2295 |
959-961 |
CD |
denotes |
12 |
T2296 |
962-965 |
VBD |
denotes |
was |
T2297 |
976-981 |
VBG |
denotes |
using |
T2298 |
982-986 |
NN |
denotes |
BALB |
T2299 |
987-988 |
NN |
denotes |
c |
T2300 |
986-987 |
HYPH |
denotes |
/ |
T2301 |
989-994 |
NN |
denotes |
mouse |
T2302 |
995-1000 |
NN |
denotes |
brain |
T2303 |
1007-1010 |
NN |
denotes |
RNA |
T2304 |
1001-1006 |
JJ |
denotes |
total |
T2305 |
1011-1014 |
CC |
denotes |
and |
T2306 |
1015-1018 |
DT |
denotes |
the |
T2307 |
1029-1037 |
NNS |
denotes |
products |
T2308 |
1019-1028 |
VBG |
denotes |
resulting |
T2309 |
1038-1047 |
VBN |
denotes |
sequenced |
T2310 |
1047-1048 |
. |
denotes |
. |
T2311 |
1048-1170 |
sentence |
denotes |
These products show that the larger transcript is due to an alternative splice event that results in an expanded exon 13. |
T2312 |
1049-1054 |
DT |
denotes |
These |
T2313 |
1055-1063 |
NNS |
denotes |
products |
T2314 |
1064-1068 |
VBP |
denotes |
show |
T2315 |
1069-1073 |
IN |
denotes |
that |
T2316 |
1096-1098 |
VBZ |
denotes |
is |
T2317 |
1074-1077 |
DT |
denotes |
the |
T2318 |
1085-1095 |
NN |
denotes |
transcript |
T2319 |
1078-1084 |
JJR |
denotes |
larger |
T2320 |
1099-1102 |
IN |
denotes |
due |
T2321 |
1103-1105 |
IN |
denotes |
to |
T2322 |
1106-1108 |
DT |
denotes |
an |
T2323 |
1128-1133 |
NN |
denotes |
event |
T2324 |
1109-1120 |
JJ |
denotes |
alternative |
T2325 |
1121-1127 |
NN |
denotes |
splice |
T2326 |
1134-1138 |
WDT |
denotes |
that |
T2327 |
1139-1146 |
VBZ |
denotes |
results |
T2328 |
1147-1149 |
IN |
denotes |
in |
T2329 |
1150-1152 |
DT |
denotes |
an |
T2330 |
1162-1166 |
NN |
denotes |
exon |
T2331 |
1153-1161 |
VBN |
denotes |
expanded |
T2332 |
1167-1169 |
CD |
denotes |
13 |
T2333 |
1169-1170 |
. |
denotes |
. |
T2334 |
1170-1292 |
sentence |
denotes |
Specifically, exon 12 splices at bp 436 of intron 12, creating a large 1614 bp exon 13 that splices correctly to exon 14. |
T2335 |
1171-1183 |
RB |
denotes |
Specifically |
T2336 |
1193-1200 |
VBZ |
denotes |
splices |
T2337 |
1183-1185 |
, |
denotes |
, |
T2338 |
1185-1189 |
NN |
denotes |
exon |
T2339 |
1190-1192 |
CD |
denotes |
12 |
T2340 |
1201-1203 |
IN |
denotes |
at |
T2341 |
1204-1206 |
NN |
denotes |
bp |
T2342 |
1207-1210 |
CD |
denotes |
436 |
T2343 |
1211-1213 |
IN |
denotes |
of |
T2344 |
1214-1220 |
NN |
denotes |
intron |
T2345 |
1221-1223 |
CD |
denotes |
12 |
T2346 |
1223-1225 |
, |
denotes |
, |
T2347 |
1225-1233 |
VBG |
denotes |
creating |
T2348 |
1234-1235 |
DT |
denotes |
a |
T2349 |
1250-1254 |
NN |
denotes |
exon |
T2350 |
1236-1241 |
JJ |
denotes |
large |
T2351 |
1242-1246 |
CD |
denotes |
1614 |
T2352 |
1247-1249 |
NN |
denotes |
bp |
T2353 |
1255-1257 |
CD |
denotes |
13 |
T2354 |
1258-1262 |
WDT |
denotes |
that |
T2355 |
1263-1270 |
VBZ |
denotes |
splices |
T2356 |
1271-1280 |
RB |
denotes |
correctly |
T2357 |
1281-1283 |
IN |
denotes |
to |
T2358 |
1284-1288 |
NN |
denotes |
exon |
T2359 |
1289-1291 |
CD |
denotes |
14 |
T2360 |
1291-1292 |
. |
denotes |
. |
T2361 |
1292-1449 |
sentence |
denotes |
The open reading frame of this alternatively spliced transcript is 611 amino acids, 28 amino acids longer than the message encoded by the 2.4 kb transcript. |
T2362 |
1293-1296 |
DT |
denotes |
The |
T2363 |
1310-1315 |
NN |
denotes |
frame |
T2364 |
1297-1301 |
JJ |
denotes |
open |
T2365 |
1302-1309 |
NN |
denotes |
reading |
T2366 |
1357-1359 |
VBZ |
denotes |
is |
T2367 |
1316-1318 |
IN |
denotes |
of |
T2368 |
1319-1323 |
DT |
denotes |
this |
T2369 |
1346-1356 |
NN |
denotes |
transcript |
T2370 |
1324-1337 |
RB |
denotes |
alternatively |
T2371 |
1338-1345 |
VBN |
denotes |
spliced |
T2372 |
1360-1363 |
CD |
denotes |
611 |
T2373 |
1370-1375 |
NNS |
denotes |
acids |
T2374 |
1364-1369 |
NN |
denotes |
amino |
T2375 |
1375-1377 |
, |
denotes |
, |
T2376 |
1377-1379 |
CD |
denotes |
28 |
T2377 |
1386-1391 |
NNS |
denotes |
acids |
T2378 |
1380-1385 |
JJ |
denotes |
amino |
T2379 |
1392-1398 |
JJR |
denotes |
longer |
T2380 |
1399-1403 |
IN |
denotes |
than |
T2381 |
1404-1407 |
DT |
denotes |
the |
T2382 |
1408-1415 |
NN |
denotes |
message |
T2383 |
1416-1423 |
VBN |
denotes |
encoded |
T2384 |
1424-1426 |
IN |
denotes |
by |
T2385 |
1427-1430 |
DT |
denotes |
the |
T2386 |
1438-1448 |
NN |
denotes |
transcript |
T2387 |
1431-1434 |
CD |
denotes |
2.4 |
T2388 |
1435-1437 |
NN |
denotes |
kb |
T2389 |
1448-1449 |
. |
denotes |
. |
T2390 |
1449-1653 |
sentence |
denotes |
TMPred analysis predicts that isoform 2 encodes a protein identical in structure to Mcoln1, possessing 6 transmembrane domains and a channel pore, however the protein sequences diverge at amino acid 526. |
T2391 |
1450-1456 |
VBN |
denotes |
TMPred |
T2392 |
1457-1465 |
NN |
denotes |
analysis |
T2393 |
1466-1474 |
VBZ |
denotes |
predicts |
T2394 |
1627-1634 |
VBP |
denotes |
diverge |
T2395 |
1475-1479 |
IN |
denotes |
that |
T2396 |
1490-1497 |
VBZ |
denotes |
encodes |
T2397 |
1480-1487 |
NN |
denotes |
isoform |
T2398 |
1488-1489 |
CD |
denotes |
2 |
T2399 |
1498-1499 |
DT |
denotes |
a |
T2400 |
1500-1507 |
NN |
denotes |
protein |
T2401 |
1508-1517 |
JJ |
denotes |
identical |
T2402 |
1518-1520 |
IN |
denotes |
in |
T2403 |
1521-1530 |
NN |
denotes |
structure |
T2404 |
1531-1533 |
IN |
denotes |
to |
T2405 |
1534-1540 |
NN |
denotes |
Mcoln1 |
T2406 |
1540-1542 |
, |
denotes |
, |
T2407 |
1542-1552 |
VBG |
denotes |
possessing |
T2408 |
1553-1554 |
CD |
denotes |
6 |
T2409 |
1569-1576 |
NNS |
denotes |
domains |
T2410 |
1555-1568 |
NN |
denotes |
transmembrane |
T2411 |
1577-1580 |
CC |
denotes |
and |
T2412 |
1581-1582 |
DT |
denotes |
a |
T2413 |
1591-1595 |
NN |
denotes |
pore |
T2414 |
1583-1590 |
NN |
denotes |
channel |
T2415 |
1595-1597 |
, |
denotes |
, |
T2416 |
1597-1604 |
RB |
denotes |
however |
T2417 |
1605-1608 |
DT |
denotes |
the |
T2418 |
1617-1626 |
NNS |
denotes |
sequences |
T2419 |
1609-1616 |
NN |
denotes |
protein |
T2420 |
1635-1637 |
IN |
denotes |
at |
T2421 |
1638-1643 |
JJ |
denotes |
amino |
T2422 |
1644-1648 |
NN |
denotes |
acid |
T2423 |
1649-1652 |
CD |
denotes |
526 |
T2424 |
1652-1653 |
. |
denotes |
. |
T2425 |
1653-1843 |
sentence |
denotes |
The 55 amino acid C-terminal cytoplasmic tail encoded by the 2.4 kb transcript is completely different from the 86 amino acid tail encoded by the murine specific 4.4 kb transcript (Fig. 4). |
T2426 |
1654-1657 |
DT |
denotes |
The |
T2427 |
1695-1699 |
NN |
denotes |
tail |
T2428 |
1658-1660 |
CD |
denotes |
55 |
T2429 |
1667-1671 |
NN |
denotes |
acid |
T2430 |
1661-1666 |
NN |
denotes |
amino |
T2431 |
1672-1673 |
NN |
denotes |
C |
T2432 |
1674-1682 |
JJ |
denotes |
terminal |
T2433 |
1673-1674 |
HYPH |
denotes |
- |
T2434 |
1683-1694 |
JJ |
denotes |
cytoplasmic |
T2435 |
1733-1735 |
VBZ |
denotes |
is |
T2436 |
1700-1707 |
VBN |
denotes |
encoded |
T2437 |
1708-1710 |
IN |
denotes |
by |
T2438 |
1711-1714 |
DT |
denotes |
the |
T2439 |
1722-1732 |
NN |
denotes |
transcript |
T2440 |
1715-1718 |
CD |
denotes |
2.4 |
T2441 |
1719-1721 |
NN |
denotes |
kb |
T2442 |
1736-1746 |
RB |
denotes |
completely |
T2443 |
1747-1756 |
JJ |
denotes |
different |
T2444 |
1757-1761 |
IN |
denotes |
from |
T2445 |
1762-1765 |
DT |
denotes |
the |
T2446 |
1780-1784 |
NN |
denotes |
tail |
T2447 |
1766-1768 |
CD |
denotes |
86 |
T2448 |
1775-1779 |
NN |
denotes |
acid |
T2449 |
1769-1774 |
NN |
denotes |
amino |
T2450 |
1785-1792 |
VBN |
denotes |
encoded |
T2451 |
1793-1795 |
IN |
denotes |
by |
T2452 |
1796-1799 |
DT |
denotes |
the |
T2453 |
1823-1833 |
NN |
denotes |
transcript |
T2454 |
1800-1806 |
NN |
denotes |
murine |
T2455 |
1807-1815 |
JJ |
denotes |
specific |
T2456 |
1816-1819 |
CD |
denotes |
4.4 |
T2457 |
1820-1822 |
NN |
denotes |
kb |
T2458 |
1834-1835 |
-LRB- |
denotes |
( |
T2459 |
1835-1839 |
NN |
denotes |
Fig. |
T2460 |
1840-1841 |
CD |
denotes |
4 |
T2461 |
1841-1842 |
-RRB- |
denotes |
) |
T2462 |
1842-1843 |
. |
denotes |
. |
T2463 |
1843-2132 |
sentence |
denotes |
Clontech Mouse RNA Master Blots were hybridized with the exon 2 and intron 12 probes mentioned above in an attempt to determine if these two transcripts showed differences in expression patterns, however, there was no significant difference in the 22 tissues represented (data not shown). |
T2464 |
1844-1852 |
NNP |
denotes |
Clontech |
T2465 |
1870-1875 |
NNPS |
denotes |
Blots |
T2466 |
1853-1858 |
NNP |
denotes |
Mouse |
T2467 |
1859-1862 |
NNP |
denotes |
RNA |
T2468 |
1863-1869 |
NNP |
denotes |
Master |
T2469 |
1881-1891 |
VBN |
denotes |
hybridized |
T2470 |
1876-1880 |
VBD |
denotes |
were |
T2471 |
2055-2058 |
VBD |
denotes |
was |
T2472 |
1892-1896 |
IN |
denotes |
with |
T2473 |
1897-1900 |
DT |
denotes |
the |
T2474 |
1922-1928 |
NNS |
denotes |
probes |
T2475 |
1901-1905 |
NN |
denotes |
exon |
T2476 |
1906-1907 |
CD |
denotes |
2 |
T2477 |
1908-1911 |
CC |
denotes |
and |
T2478 |
1912-1918 |
NN |
denotes |
intron |
T2479 |
1919-1921 |
CD |
denotes |
12 |
T2480 |
1929-1938 |
VBN |
denotes |
mentioned |
T2481 |
1939-1944 |
RB |
denotes |
above |
T2482 |
1945-1947 |
IN |
denotes |
in |
T2483 |
1948-1950 |
DT |
denotes |
an |
T2484 |
1951-1958 |
NN |
denotes |
attempt |
T2485 |
1959-1961 |
TO |
denotes |
to |
T2486 |
1962-1971 |
VB |
denotes |
determine |
T2487 |
1972-1974 |
IN |
denotes |
if |
T2488 |
1997-2003 |
VBD |
denotes |
showed |
T2489 |
1975-1980 |
DT |
denotes |
these |
T2490 |
1985-1996 |
NNS |
denotes |
transcripts |
T2491 |
1981-1984 |
CD |
denotes |
two |
T2492 |
2004-2015 |
NNS |
denotes |
differences |
T2493 |
2016-2018 |
IN |
denotes |
in |
T2494 |
2019-2029 |
NN |
denotes |
expression |
T2495 |
2030-2038 |
NNS |
denotes |
patterns |
T2496 |
2038-2040 |
, |
denotes |
, |
T2497 |
2040-2047 |
RB |
denotes |
however |
T2498 |
2047-2049 |
, |
denotes |
, |
T2499 |
2049-2054 |
EX |
denotes |
there |
T2500 |
2059-2061 |
DT |
denotes |
no |
T2501 |
2074-2084 |
NN |
denotes |
difference |
T2502 |
2062-2073 |
JJ |
denotes |
significant |
T2503 |
2085-2087 |
IN |
denotes |
in |
T2504 |
2088-2091 |
DT |
denotes |
the |
T2505 |
2095-2102 |
NNS |
denotes |
tissues |
T2506 |
2092-2094 |
CD |
denotes |
22 |
T2507 |
2103-2114 |
VBN |
denotes |
represented |
T2508 |
2115-2116 |
-LRB- |
denotes |
( |
T2509 |
2125-2130 |
VBN |
denotes |
shown |
T2510 |
2116-2120 |
NNS |
denotes |
data |
T2511 |
2121-2124 |
RB |
denotes |
not |
T2512 |
2130-2131 |
-RRB- |
denotes |
) |
T2513 |
2131-2132 |
. |
denotes |
. |
T2514 |
2132-2532 |
sentence |
denotes |
Figure 4 Peptide sequence comparison of the two alternatively spliced Mcoln1 isoforms. The green box surrounds the divergent c-terminal cytoplasmic tails. The blue lines indicate the transmembrane domains. Next, we directly compared the nucleotide and amino acid sequence of the alternatively spliced mouse transcript to the entire human MCOLN1 genomic sequence and found no significant similarity. |
T2515 |
2340-2344 |
RB |
denotes |
Next |
T2516 |
2358-2366 |
VBD |
denotes |
compared |
T2517 |
2344-2346 |
, |
denotes |
, |
T2518 |
2346-2348 |
PRP |
denotes |
we |
T2519 |
2349-2357 |
RB |
denotes |
directly |
T2520 |
2367-2370 |
DT |
denotes |
the |
T2521 |
2397-2405 |
NN |
denotes |
sequence |
T2522 |
2371-2381 |
NN |
denotes |
nucleotide |
T2523 |
2382-2385 |
CC |
denotes |
and |
T2524 |
2386-2391 |
NN |
denotes |
amino |
T2525 |
2392-2396 |
NN |
denotes |
acid |
T2526 |
2406-2408 |
IN |
denotes |
of |
T2527 |
2409-2412 |
DT |
denotes |
the |
T2528 |
2441-2451 |
NN |
denotes |
transcript |
T2529 |
2413-2426 |
RB |
denotes |
alternatively |
T2530 |
2427-2434 |
VBN |
denotes |
spliced |
T2531 |
2435-2440 |
NN |
denotes |
mouse |
T2532 |
2452-2454 |
IN |
denotes |
to |
T2533 |
2455-2458 |
DT |
denotes |
the |
T2534 |
2487-2495 |
NN |
denotes |
sequence |
T2535 |
2459-2465 |
JJ |
denotes |
entire |
T2536 |
2466-2471 |
JJ |
denotes |
human |
T2537 |
2472-2478 |
NN |
denotes |
MCOLN1 |
T2538 |
2479-2486 |
JJ |
denotes |
genomic |
T2539 |
2496-2499 |
CC |
denotes |
and |
T2540 |
2500-2505 |
VBD |
denotes |
found |
T2541 |
2506-2508 |
DT |
denotes |
no |
T2542 |
2521-2531 |
NN |
denotes |
similarity |
T2543 |
2509-2520 |
JJ |
denotes |
significant |
T2544 |
2531-2532 |
. |
denotes |
. |
T2545 |
2532-2640 |
sentence |
denotes |
As mentioned previously, Northern blots performed with human MCOLN1 probes show only one 2.4 kb transcript. |
T2546 |
2533-2535 |
IN |
denotes |
As |
T2547 |
2536-2545 |
VBN |
denotes |
mentioned |
T2548 |
2608-2612 |
VBP |
denotes |
show |
T2549 |
2546-2556 |
RB |
denotes |
previously |
T2550 |
2556-2558 |
, |
denotes |
, |
T2551 |
2558-2566 |
NNP |
denotes |
Northern |
T2552 |
2567-2572 |
NNS |
denotes |
blots |
T2553 |
2573-2582 |
VBN |
denotes |
performed |
T2554 |
2583-2587 |
IN |
denotes |
with |
T2555 |
2588-2593 |
JJ |
denotes |
human |
T2556 |
2594-2600 |
NN |
denotes |
MCOLN1 |
T2557 |
2601-2607 |
NNS |
denotes |
probes |
T2558 |
2613-2617 |
RB |
denotes |
only |
T2559 |
2629-2639 |
NN |
denotes |
transcript |
T2560 |
2618-2621 |
CD |
denotes |
one |
T2561 |
2622-2625 |
CD |
denotes |
2.4 |
T2562 |
2626-2628 |
NN |
denotes |
kb |
T2563 |
2639-2640 |
. |
denotes |
. |
T2564 |
2640-2780 |
sentence |
denotes |
In addition, we hybridized a human multiple tissue Northern and human Southern with a probe in human intron 12 that is adjacent to exon 13. |
T2565 |
2641-2643 |
IN |
denotes |
In |
T2566 |
2657-2667 |
VBD |
denotes |
hybridized |
T2567 |
2644-2652 |
NN |
denotes |
addition |
T2568 |
2652-2654 |
, |
denotes |
, |
T2569 |
2654-2656 |
PRP |
denotes |
we |
T2570 |
2668-2669 |
DT |
denotes |
a |
T2571 |
2692-2700 |
NNP |
denotes |
Northern |
T2572 |
2670-2675 |
JJ |
denotes |
human |
T2573 |
2676-2684 |
JJ |
denotes |
multiple |
T2574 |
2685-2691 |
NN |
denotes |
tissue |
T2575 |
2701-2704 |
CC |
denotes |
and |
T2576 |
2705-2710 |
JJ |
denotes |
human |
T2577 |
2711-2719 |
NNP |
denotes |
Southern |
T2578 |
2720-2724 |
IN |
denotes |
with |
T2579 |
2725-2726 |
DT |
denotes |
a |
T2580 |
2727-2732 |
NN |
denotes |
probe |
T2581 |
2733-2735 |
IN |
denotes |
in |
T2582 |
2736-2741 |
JJ |
denotes |
human |
T2583 |
2742-2748 |
NN |
denotes |
intron |
T2584 |
2749-2751 |
CD |
denotes |
12 |
T2585 |
2752-2756 |
WDT |
denotes |
that |
T2586 |
2757-2759 |
VBZ |
denotes |
is |
T2587 |
2760-2768 |
JJ |
denotes |
adjacent |
T2588 |
2769-2771 |
IN |
denotes |
to |
T2589 |
2772-2776 |
NN |
denotes |
exon |
T2590 |
2777-2779 |
CD |
denotes |
13 |
T2591 |
2779-2780 |
. |
denotes |
. |
T2592 |
2780-2879 |
sentence |
denotes |
The probe was located in the region syntenic to that which encodes the alternate mouse transcript. |
T2593 |
2781-2784 |
DT |
denotes |
The |
T2594 |
2785-2790 |
NN |
denotes |
probe |
T2595 |
2795-2802 |
VBN |
denotes |
located |
T2596 |
2791-2794 |
VBD |
denotes |
was |
T2597 |
2803-2805 |
IN |
denotes |
in |
T2598 |
2806-2809 |
DT |
denotes |
the |
T2599 |
2810-2816 |
NN |
denotes |
region |
T2600 |
2817-2825 |
JJ |
denotes |
syntenic |
T2601 |
2826-2828 |
IN |
denotes |
to |
T2602 |
2829-2833 |
DT |
denotes |
that |
T2603 |
2834-2839 |
WDT |
denotes |
which |
T2604 |
2840-2847 |
VBZ |
denotes |
encodes |
T2605 |
2848-2851 |
DT |
denotes |
the |
T2606 |
2868-2878 |
NN |
denotes |
transcript |
T2607 |
2852-2861 |
JJ |
denotes |
alternate |
T2608 |
2862-2867 |
NN |
denotes |
mouse |
T2609 |
2878-2879 |
. |
denotes |
. |
T2610 |
2879-3051 |
sentence |
denotes |
Only the expected bands were detected on the Southern and no bands were detected on the Northern, confirming that this alternative transcript is specific to murine Mcoln1. |
T2611 |
2880-2884 |
RB |
denotes |
Only |
T2612 |
2898-2903 |
NNS |
denotes |
bands |
T2613 |
2885-2888 |
DT |
denotes |
the |
T2614 |
2889-2897 |
VBN |
denotes |
expected |
T2615 |
2909-2917 |
VBN |
denotes |
detected |
T2616 |
2904-2908 |
VBD |
denotes |
were |
T2617 |
2918-2920 |
IN |
denotes |
on |
T2618 |
2921-2924 |
DT |
denotes |
the |
T2619 |
2925-2933 |
NNP |
denotes |
Southern |
T2620 |
2934-2937 |
CC |
denotes |
and |
T2621 |
2938-2940 |
DT |
denotes |
no |
T2622 |
2941-2946 |
NNS |
denotes |
bands |
T2623 |
2952-2960 |
VBN |
denotes |
detected |
T2624 |
2947-2951 |
VBD |
denotes |
were |
T2625 |
2961-2963 |
IN |
denotes |
on |
T2626 |
2964-2967 |
DT |
denotes |
the |
T2627 |
2968-2976 |
NNP |
denotes |
Northern |
T2628 |
2976-2978 |
, |
denotes |
, |
T2629 |
2978-2988 |
VBG |
denotes |
confirming |
T2630 |
2989-2993 |
IN |
denotes |
that |
T2631 |
3022-3024 |
VBZ |
denotes |
is |
T2632 |
2994-2998 |
DT |
denotes |
this |
T2633 |
3011-3021 |
NN |
denotes |
transcript |
T2634 |
2999-3010 |
JJ |
denotes |
alternative |
T2635 |
3025-3033 |
JJ |
denotes |
specific |
T2636 |
3034-3036 |
IN |
denotes |
to |
T2637 |
3037-3043 |
JJ |
denotes |
murine |
T2638 |
3044-3050 |
NN |
denotes |
Mcoln1 |
T2639 |
3050-3051 |
. |
denotes |
. |
T2640 |
3051-3219 |
sentence |
denotes |
Recent BLASTP analysis of the alternate Mcoln1 transcript yields a match to a putative 145 amino acid anonymous protein (GB No. BAB25862) predicted from a RIKEN clone. |
T2641 |
3052-3058 |
JJ |
denotes |
Recent |
T2642 |
3066-3074 |
NN |
denotes |
analysis |
T2643 |
3059-3065 |
NN |
denotes |
BLASTP |
T2644 |
3110-3116 |
VBZ |
denotes |
yields |
T2645 |
3075-3077 |
IN |
denotes |
of |
T2646 |
3078-3081 |
DT |
denotes |
the |
T2647 |
3099-3109 |
NN |
denotes |
transcript |
T2648 |
3082-3091 |
JJ |
denotes |
alternate |
T2649 |
3092-3098 |
NN |
denotes |
Mcoln1 |
T2650 |
3117-3118 |
DT |
denotes |
a |
T2651 |
3119-3124 |
NN |
denotes |
match |
T2652 |
3125-3127 |
IN |
denotes |
to |
T2653 |
3128-3129 |
DT |
denotes |
a |
T2654 |
3164-3171 |
NN |
denotes |
protein |
T2655 |
3130-3138 |
JJ |
denotes |
putative |
T2656 |
3139-3142 |
CD |
denotes |
145 |
T2657 |
3149-3153 |
NN |
denotes |
acid |
T2658 |
3143-3148 |
NN |
denotes |
amino |
T2659 |
3154-3163 |
JJ |
denotes |
anonymous |
T2660 |
3172-3173 |
-LRB- |
denotes |
( |
T2661 |
3180-3188 |
NN |
denotes |
BAB25862 |
T2662 |
3173-3175 |
NN |
denotes |
GB |
T2663 |
3176-3179 |
NN |
denotes |
No. |
T2664 |
3188-3189 |
-RRB- |
denotes |
) |
T2665 |
3190-3199 |
VBN |
denotes |
predicted |
T2666 |
3200-3204 |
IN |
denotes |
from |
T2667 |
3205-3206 |
DT |
denotes |
a |
T2668 |
3213-3218 |
NN |
denotes |
clone |
T2669 |
3207-3212 |
NN |
denotes |
RIKEN |
T2670 |
3218-3219 |
. |
denotes |
. |
T2671 |
3219-3461 |
sentence |
denotes |
It is obvious from our results, however, that the identification of this sequence as a full-length protein is incorrect since probes unique to the clone, as well as probes containing the Mcoln1 coding sequence, identify the same transcripts. |
T2672 |
3220-3222 |
PRP |
denotes |
It |
T2673 |
3223-3225 |
VBZ |
denotes |
is |
T2674 |
3226-3233 |
JJ |
denotes |
obvious |
T2675 |
3234-3238 |
IN |
denotes |
from |
T2676 |
3239-3242 |
PRP$ |
denotes |
our |
T2677 |
3243-3250 |
NNS |
denotes |
results |
T2678 |
3250-3252 |
, |
denotes |
, |
T2679 |
3252-3259 |
RB |
denotes |
however |
T2680 |
3259-3261 |
, |
denotes |
, |
T2681 |
3261-3265 |
IN |
denotes |
that |
T2682 |
3327-3329 |
VBZ |
denotes |
is |
T2683 |
3266-3269 |
DT |
denotes |
the |
T2684 |
3270-3284 |
NN |
denotes |
identification |
T2685 |
3285-3287 |
IN |
denotes |
of |
T2686 |
3288-3292 |
DT |
denotes |
this |
T2687 |
3293-3301 |
NN |
denotes |
sequence |
T2688 |
3302-3304 |
IN |
denotes |
as |
T2689 |
3305-3306 |
DT |
denotes |
a |
T2690 |
3319-3326 |
NN |
denotes |
protein |
T2691 |
3307-3311 |
JJ |
denotes |
full |
T2692 |
3312-3318 |
NN |
denotes |
length |
T2693 |
3311-3312 |
HYPH |
denotes |
- |
T2694 |
3330-3339 |
JJ |
denotes |
incorrect |
T2695 |
3340-3345 |
IN |
denotes |
since |
T2696 |
3431-3439 |
VBP |
denotes |
identify |
T2697 |
3346-3352 |
NNS |
denotes |
probes |
T2698 |
3353-3359 |
JJ |
denotes |
unique |
T2699 |
3360-3362 |
IN |
denotes |
to |
T2700 |
3363-3366 |
DT |
denotes |
the |
T2701 |
3367-3372 |
NN |
denotes |
clone |
T2702 |
3372-3374 |
, |
denotes |
, |
T2703 |
3374-3376 |
RB |
denotes |
as |
T2704 |
3382-3384 |
IN |
denotes |
as |
T2705 |
3377-3381 |
RB |
denotes |
well |
T2706 |
3385-3391 |
NNS |
denotes |
probes |
T2707 |
3392-3402 |
VBG |
denotes |
containing |
T2708 |
3403-3406 |
DT |
denotes |
the |
T2709 |
3421-3429 |
NN |
denotes |
sequence |
T2710 |
3407-3413 |
NN |
denotes |
Mcoln1 |
T2711 |
3414-3420 |
NN |
denotes |
coding |
T2712 |
3429-3431 |
, |
denotes |
, |
T2713 |
3440-3443 |
DT |
denotes |
the |
T2714 |
3449-3460 |
NNS |
denotes |
transcripts |
T2715 |
3444-3448 |
JJ |
denotes |
same |
T2716 |
3460-3461 |
. |
denotes |
. |
T4631 |
2143-2150 |
NN |
denotes |
Peptide |
T4632 |
2151-2159 |
NN |
denotes |
sequence |
T4633 |
2160-2170 |
NN |
denotes |
comparison |
T4634 |
2171-2173 |
IN |
denotes |
of |
T4635 |
2174-2177 |
DT |
denotes |
the |
T4636 |
2211-2219 |
NNS |
denotes |
isoforms |
T4637 |
2178-2181 |
CD |
denotes |
two |
T4638 |
2182-2195 |
RB |
denotes |
alternatively |
T4639 |
2196-2203 |
VBN |
denotes |
spliced |
T4640 |
2204-2210 |
NN |
denotes |
Mcoln1 |
T4641 |
2219-2220 |
. |
denotes |
. |
T4642 |
2220-2288 |
sentence |
denotes |
The green box surrounds the divergent c-terminal cytoplasmic tails. |
T4643 |
2221-2224 |
DT |
denotes |
The |
T4644 |
2231-2234 |
NN |
denotes |
box |
T4645 |
2225-2230 |
JJ |
denotes |
green |
T4646 |
2235-2244 |
VBZ |
denotes |
surrounds |
T4647 |
2245-2248 |
DT |
denotes |
the |
T4648 |
2282-2287 |
NNS |
denotes |
tails |
T4649 |
2249-2258 |
JJ |
denotes |
divergent |
T4650 |
2259-2260 |
NN |
denotes |
c |
T4651 |
2261-2269 |
JJ |
denotes |
terminal |
T4652 |
2260-2261 |
HYPH |
denotes |
- |
T4653 |
2270-2281 |
JJ |
denotes |
cytoplasmic |
T4654 |
2287-2288 |
. |
denotes |
. |
T4655 |
2288-2339 |
sentence |
denotes |
The blue lines indicate the transmembrane domains. |
T4656 |
2289-2292 |
DT |
denotes |
The |
T4657 |
2298-2303 |
NNS |
denotes |
lines |
T4658 |
2293-2297 |
JJ |
denotes |
blue |
T4659 |
2304-2312 |
VBP |
denotes |
indicate |
T4660 |
2313-2316 |
DT |
denotes |
the |
T4661 |
2331-2338 |
NNS |
denotes |
domains |
T4662 |
2317-2330 |
NN |
denotes |
transmembrane |
T4663 |
2338-2339 |
. |
denotes |
. |
R1200 |
T2106 |
T2105 |
prep |
of,Characterization |
R1201 |
T2107 |
T2108 |
det |
the,variant |
R1202 |
T2108 |
T2106 |
pobj |
variant,of |
R1203 |
T2109 |
T2108 |
nmod |
Mcoln1,variant |
R1204 |
T2110 |
T2111 |
amod |
alternative,splice |
R1205 |
T2111 |
T2108 |
compound |
splice,variant |
R1206 |
T2113 |
T2114 |
prep |
In,searched |
R1207 |
T2115 |
T2113 |
pobj |
order,In |
R1208 |
T2116 |
T2117 |
aux |
to,determine |
R1209 |
T2117 |
T2115 |
acl |
determine,order |
R1210 |
T2118 |
T2119 |
det |
the,sequence |
R1211 |
T2119 |
T2117 |
dobj |
sequence,determine |
R1212 |
T2120 |
T2119 |
amod |
coding,sequence |
R1213 |
T2121 |
T2119 |
prep |
for,sequence |
R1214 |
T2122 |
T2123 |
det |
the,transcript |
R1215 |
T2123 |
T2121 |
pobj |
transcript,for |
R1216 |
T2124 |
T2123 |
amod |
larger,transcript |
R1217 |
T2125 |
T2114 |
punct |
", ",searched |
R1218 |
T2126 |
T2114 |
nsubj |
we,searched |
R1219 |
T2127 |
T2128 |
det |
the,database |
R1220 |
T2128 |
T2114 |
dobj |
database,searched |
R1221 |
T2129 |
T2130 |
compound |
mouse,EST |
R1222 |
T2130 |
T2128 |
compound |
EST,database |
R1223 |
T2131 |
T2114 |
advcl |
using,searched |
R1224 |
T2132 |
T2133 |
det |
each,intron |
R1225 |
T2133 |
T2131 |
dobj |
intron,using |
R1226 |
T2134 |
T2135 |
advmod |
as,as |
R1227 |
T2135 |
T2133 |
cc |
as,intron |
R1228 |
T2136 |
T2135 |
advmod |
well,as |
R1229 |
T2137 |
T2138 |
det |
the,sequence |
R1230 |
T2138 |
T2133 |
conj |
sequence,intron |
R1231 |
T2139 |
T2138 |
amod |
genomic,sequence |
R1232 |
T2140 |
T2138 |
acl |
flanking,sequence |
R1233 |
T2141 |
T2142 |
det |
the,gene |
R1234 |
T2142 |
T2140 |
dobj |
gene,flanking |
R1235 |
T2143 |
T2142 |
compound |
Mcoln1,gene |
R1236 |
T2144 |
T2114 |
punct |
.,searched |
R1237 |
T2146 |
T2147 |
nummod |
Two,ESTs |
R1238 |
T2147 |
T2148 |
nsubjpass |
ESTs,identified |
R1239 |
T2149 |
T2148 |
auxpass |
were,identified |
R1240 |
T2150 |
T2151 |
dep |
that,contained |
R1241 |
T2151 |
T2148 |
ccomp |
contained,identified |
R1242 |
T2152 |
T2151 |
dobj |
sequence,contained |
R1243 |
T2153 |
T2152 |
prep |
from,sequence |
R1244 |
T2154 |
T2153 |
pobj |
intron,from |
R1245 |
T2155 |
T2154 |
nummod |
12,intron |
R1246 |
T2156 |
T2157 |
punct |
(,AI430291 |
R1247 |
T2157 |
T2148 |
parataxis |
AI430291,identified |
R1248 |
T2158 |
T2157 |
compound |
GB,AI430291 |
R1249 |
T2159 |
T2157 |
compound |
No.,AI430291 |
R1250 |
T2160 |
T2157 |
cc |
and,AI430291 |
R1251 |
T2161 |
T2157 |
conj |
AA874645,AI430291 |
R1252 |
T2162 |
T2157 |
punct |
),AI430291 |
R1253 |
T2163 |
T2148 |
punct |
", ",identified |
R1254 |
T2164 |
T2148 |
cc |
and,identified |
R1255 |
T2165 |
T2166 |
det |
the,clones |
R1256 |
T2166 |
T2168 |
nsubjpass |
clones,sequenced |
R1257 |
T2167 |
T2166 |
amod |
corresponding,clones |
R1258 |
T2168 |
T2148 |
conj |
sequenced,identified |
R1259 |
T2169 |
T2168 |
auxpass |
were,sequenced |
R1260 |
T2170 |
T2168 |
punct |
.,sequenced |
R1261 |
T2172 |
T2173 |
nsubj |
Clone,begins |
R1262 |
T2174 |
T2172 |
nummod |
408619,Clone |
R1263 |
T2175 |
T2176 |
punct |
(,AI429558 |
R1264 |
T2176 |
T2172 |
parataxis |
AI429558,Clone |
R1265 |
T2177 |
T2176 |
dep |
ESTs,AI429558 |
R1266 |
T2178 |
T2176 |
punct |
: ,AI429558 |
R1267 |
T2179 |
T2176 |
nmod |
GB,AI429558 |
R1268 |
T2180 |
T2176 |
nmod |
No.,AI429558 |
R1269 |
T2181 |
T2176 |
nmod |
AI430291,AI429558 |
R1270 |
T2182 |
T2176 |
punct |
", ",AI429558 |
R1271 |
T2183 |
T2176 |
punct |
),AI429558 |
R1272 |
T2184 |
T2185 |
advmod |
approximately,1.1 |
R1273 |
T2185 |
T2186 |
nummod |
1.1,kb |
R1274 |
T2186 |
T2187 |
npadvmod |
kb,before |
R1275 |
T2187 |
T2173 |
prep |
before,begins |
R1276 |
T2188 |
T2187 |
pobj |
exon,before |
R1277 |
T2189 |
T2188 |
nummod |
13,exon |
R1278 |
T2190 |
T2173 |
cc |
and,begins |
R1279 |
T2191 |
T2173 |
conj |
continues,begins |
R1280 |
T2192 |
T2191 |
prep |
through,continues |
R1281 |
T2193 |
T2194 |
det |
the,exon |
R1282 |
T2194 |
T2192 |
pobj |
exon,through |
R1283 |
T2195 |
T2191 |
cc |
and,continues |
R1284 |
T2196 |
T2191 |
conj |
splices,continues |
R1285 |
T2197 |
T2196 |
advmod |
correctly,splices |
R1286 |
T2198 |
T2196 |
prep |
to,splices |
R1287 |
T2199 |
T2198 |
pobj |
exon,to |
R1288 |
T2200 |
T2199 |
nummod |
14,exon |
R1289 |
T2201 |
T2173 |
punct |
.,begins |
R1290 |
T2203 |
T2204 |
nsubj |
Clone,begins |
R1291 |
T2205 |
T2203 |
nummod |
1281641,Clone |
R1292 |
T2206 |
T2207 |
punct |
(,AA874645 |
R1293 |
T2207 |
T2203 |
parataxis |
AA874645,Clone |
R1294 |
T2208 |
T2207 |
dep |
EST,AA874645 |
R1295 |
T2209 |
T2207 |
punct |
:,AA874645 |
R1296 |
T2210 |
T2207 |
compound |
GB,AA874645 |
R1297 |
T2211 |
T2207 |
compound |
No.,AA874645 |
R1298 |
T2212 |
T2207 |
punct |
),AA874645 |
R1299 |
T2213 |
T2214 |
nummod |
175,bp |
R1300 |
T2214 |
T2215 |
npadvmod |
bp,before |
R1301 |
T2215 |
T2204 |
prep |
before,begins |
R1302 |
T2216 |
T2215 |
pobj |
exon,before |
R1303 |
T2217 |
T2216 |
nummod |
13,exon |
R1304 |
T2218 |
T2204 |
cc |
and,begins |
R1305 |
T2219 |
T2220 |
advmod |
also,splices |
R1306 |
T2220 |
T2204 |
conj |
splices,begins |
R1307 |
T2221 |
T2220 |
advmod |
correctly,splices |
R1308 |
T2222 |
T2220 |
prep |
to,splices |
R1309 |
T2223 |
T2222 |
pobj |
exon,to |
R1310 |
T2224 |
T2223 |
nummod |
14,exon |
R1311 |
T2225 |
T2204 |
punct |
.,begins |
R1312 |
T2227 |
T2228 |
det |
A,Northern |
R1313 |
T2228 |
T2232 |
nsubjpass |
Northern,hybridized |
R1314 |
T2229 |
T2228 |
nmod |
mouse,Northern |
R1315 |
T2230 |
T2231 |
amod |
multiple,tissue |
R1316 |
T2231 |
T2228 |
compound |
tissue,Northern |
R1317 |
T2233 |
T2232 |
auxpass |
was,hybridized |
R1318 |
T2234 |
T2232 |
advcl |
using,hybridized |
R1319 |
T2235 |
T2236 |
det |
a,probe |
R1320 |
T2236 |
T2234 |
dobj |
probe,using |
R1321 |
T2237 |
T2236 |
acl |
generated,probe |
R1322 |
T2238 |
T2237 |
prep |
from,generated |
R1323 |
T2239 |
T2240 |
det |
the,sequence |
R1324 |
T2240 |
T2238 |
pobj |
sequence,from |
R1325 |
T2241 |
T2240 |
amod |
putative,sequence |
R1326 |
T2242 |
T2240 |
compound |
intron,sequence |
R1327 |
T2243 |
T2240 |
prep |
in,sequence |
R1328 |
T2244 |
T2243 |
pobj |
clone,in |
R1329 |
T2245 |
T2244 |
nummod |
408619,clone |
R1330 |
T2246 |
T2247 |
punct |
(,1B |
R1331 |
T2247 |
T2236 |
parataxis |
1B,probe |
R1332 |
T2248 |
T2247 |
dep |
Probe,1B |
R1333 |
T2249 |
T2248 |
nummod |
2,Probe |
R1334 |
T2250 |
T2247 |
punct |
", ",1B |
R1335 |
T2251 |
T2247 |
compound |
Fig.,1B |
R1336 |
T2252 |
T2247 |
punct |
),1B |
R1337 |
T2253 |
T2236 |
punct |
", ",probe |
R1338 |
T2254 |
T2255 |
dep |
which,detected |
R1339 |
T2255 |
T2236 |
relcl |
detected,probe |
R1340 |
T2256 |
T2257 |
advmod |
only,band |
R1341 |
T2257 |
T2255 |
dobj |
band,detected |
R1342 |
T2258 |
T2257 |
det |
the,band |
R1343 |
T2259 |
T2260 |
nummod |
4.4,kb |
R1344 |
T2260 |
T2257 |
compound |
kb,band |
R1345 |
T2261 |
T2262 |
punct |
(,3C |
R1346 |
T2262 |
T2255 |
parataxis |
3C,detected |
R1347 |
T2263 |
T2262 |
compound |
Fig.,3C |
R1348 |
T2264 |
T2262 |
punct |
),3C |
R1349 |
T2265 |
T2232 |
punct |
.,hybridized |
R1350 |
T2267 |
T2268 |
prep |
In,performed |
R1351 |
T2269 |
T2267 |
pobj |
order,In |
R1352 |
T2270 |
T2271 |
aux |
to,determine |
R1353 |
T2271 |
T2269 |
acl |
determine,order |
R1354 |
T2272 |
T2273 |
det |
the,sequence |
R1355 |
T2273 |
T2271 |
dobj |
sequence,determine |
R1356 |
T2274 |
T2273 |
prep |
of,sequence |
R1357 |
T2275 |
T2276 |
det |
the,transcript |
R1358 |
T2276 |
T2274 |
pobj |
transcript,of |
R1359 |
T2277 |
T2276 |
amod |
entire,transcript |
R1360 |
T2278 |
T2268 |
punct |
", ",performed |
R1361 |
T2279 |
T2280 |
compound |
RT,PCR |
R1362 |
T2280 |
T2268 |
nsubjpass |
PCR,performed |
R1363 |
T2281 |
T2280 |
punct |
-,PCR |
R1364 |
T2282 |
T2280 |
acl |
using,PCR |
R1365 |
T2283 |
T2282 |
dobj |
primers,using |
R1366 |
T2284 |
T2283 |
prep |
in,primers |
R1367 |
T2285 |
T2286 |
nmod |
exons,10 |
R1368 |
T2286 |
T2284 |
pobj |
10,in |
R1369 |
T2287 |
T2286 |
cc |
and,10 |
R1370 |
T2288 |
T2286 |
conj |
11,10 |
R1371 |
T2289 |
T2283 |
acl |
paired,primers |
R1372 |
T2290 |
T2289 |
prep |
with,paired |
R1373 |
T2291 |
T2292 |
det |
a,primer |
R1374 |
T2292 |
T2290 |
pobj |
primer,with |
R1375 |
T2293 |
T2292 |
prep |
in,primer |
R1376 |
T2294 |
T2293 |
pobj |
intron,in |
R1377 |
T2295 |
T2294 |
nummod |
12,intron |
R1378 |
T2296 |
T2268 |
auxpass |
was,performed |
R1379 |
T2297 |
T2268 |
advcl |
using,performed |
R1380 |
T2298 |
T2299 |
nmod |
BALB,c |
R1381 |
T2299 |
T2301 |
nmod |
c,mouse |
R1382 |
T2300 |
T2299 |
punct |
/,c |
R1383 |
T2301 |
T2302 |
nmod |
mouse,brain |
R1384 |
T2302 |
T2303 |
nmod |
brain,RNA |
R1385 |
T2303 |
T2297 |
dobj |
RNA,using |
R1386 |
T2304 |
T2303 |
amod |
total,RNA |
R1387 |
T2305 |
T2268 |
cc |
and,performed |
R1388 |
T2306 |
T2307 |
det |
the,products |
R1389 |
T2307 |
T2309 |
nsubj |
products,sequenced |
R1390 |
T2308 |
T2307 |
amod |
resulting,products |
R1391 |
T2309 |
T2268 |
conj |
sequenced,performed |
R1392 |
T2310 |
T2268 |
punct |
.,performed |
R1393 |
T2312 |
T2313 |
det |
These,products |
R1394 |
T2313 |
T2314 |
nsubj |
products,show |
R1395 |
T2315 |
T2316 |
mark |
that,is |
R1396 |
T2316 |
T2314 |
ccomp |
is,show |
R1397 |
T2317 |
T2318 |
det |
the,transcript |
R1398 |
T2318 |
T2316 |
nsubj |
transcript,is |
R1399 |
T2319 |
T2318 |
amod |
larger,transcript |
R1400 |
T2320 |
T2316 |
prep |
due,is |
R1401 |
T2321 |
T2320 |
pcomp |
to,due |
R1402 |
T2322 |
T2323 |
det |
an,event |
R1403 |
T2323 |
T2320 |
pobj |
event,due |
R1404 |
T2324 |
T2323 |
amod |
alternative,event |
R1405 |
T2325 |
T2323 |
compound |
splice,event |
R1406 |
T2326 |
T2327 |
dep |
that,results |
R1407 |
T2327 |
T2323 |
relcl |
results,event |
R1408 |
T2328 |
T2327 |
prep |
in,results |
R1409 |
T2329 |
T2330 |
det |
an,exon |
R1410 |
T2330 |
T2328 |
pobj |
exon,in |
R1411 |
T2331 |
T2330 |
amod |
expanded,exon |
R1412 |
T2332 |
T2330 |
nummod |
13,exon |
R1413 |
T2333 |
T2314 |
punct |
.,show |
R1414 |
T2335 |
T2336 |
advmod |
Specifically,splices |
R1415 |
T2337 |
T2336 |
punct |
", ",splices |
R1416 |
T2338 |
T2336 |
nsubj |
exon,splices |
R1417 |
T2339 |
T2338 |
nummod |
12,exon |
R1418 |
T2340 |
T2336 |
prep |
at,splices |
R1419 |
T2341 |
T2340 |
pobj |
bp,at |
R1420 |
T2342 |
T2341 |
nummod |
436,bp |
R1421 |
T2343 |
T2341 |
prep |
of,bp |
R1422 |
T2344 |
T2343 |
pobj |
intron,of |
R1423 |
T2345 |
T2344 |
nummod |
12,intron |
R1424 |
T2346 |
T2336 |
punct |
", ",splices |
R1425 |
T2347 |
T2336 |
advcl |
creating,splices |
R1426 |
T2348 |
T2349 |
det |
a,exon |
R1427 |
T2349 |
T2347 |
dobj |
exon,creating |
R1428 |
T2350 |
T2349 |
amod |
large,exon |
R1429 |
T2351 |
T2352 |
nummod |
1614,bp |
R1430 |
T2352 |
T2349 |
compound |
bp,exon |
R1431 |
T2353 |
T2349 |
nummod |
13,exon |
R1432 |
T2354 |
T2355 |
dep |
that,splices |
R1433 |
T2355 |
T2349 |
relcl |
splices,exon |
R1434 |
T2356 |
T2355 |
advmod |
correctly,splices |
R1435 |
T2357 |
T2355 |
prep |
to,splices |
R1436 |
T2358 |
T2357 |
pobj |
exon,to |
R1437 |
T2359 |
T2358 |
nummod |
14,exon |
R1438 |
T2360 |
T2336 |
punct |
.,splices |
R1439 |
T2362 |
T2363 |
det |
The,frame |
R1440 |
T2363 |
T2366 |
nsubj |
frame,is |
R1441 |
T2364 |
T2363 |
amod |
open,frame |
R1442 |
T2365 |
T2363 |
compound |
reading,frame |
R1443 |
T2367 |
T2363 |
prep |
of,frame |
R1444 |
T2368 |
T2369 |
det |
this,transcript |
R1445 |
T2369 |
T2367 |
pobj |
transcript,of |
R1446 |
T2370 |
T2371 |
advmod |
alternatively,spliced |
R1447 |
T2371 |
T2369 |
amod |
spliced,transcript |
R1448 |
T2372 |
T2373 |
nummod |
611,acids |
R1449 |
T2373 |
T2366 |
attr |
acids,is |
R1450 |
T2374 |
T2373 |
compound |
amino,acids |
R1451 |
T2375 |
T2373 |
punct |
", ",acids |
R1452 |
T2376 |
T2377 |
nummod |
28,acids |
R1453 |
T2377 |
T2379 |
npadvmod |
acids,longer |
R1454 |
T2378 |
T2377 |
amod |
amino,acids |
R1455 |
T2379 |
T2373 |
amod |
longer,acids |
R1456 |
T2380 |
T2379 |
prep |
than,longer |
R1457 |
T2381 |
T2382 |
det |
the,message |
R1458 |
T2382 |
T2380 |
pobj |
message,than |
R1459 |
T2383 |
T2382 |
acl |
encoded,message |
R1460 |
T2384 |
T2383 |
agent |
by,encoded |
R1461 |
T2385 |
T2386 |
det |
the,transcript |
R1462 |
T2386 |
T2384 |
pobj |
transcript,by |
R1463 |
T2387 |
T2388 |
nummod |
2.4,kb |
R1464 |
T2388 |
T2386 |
compound |
kb,transcript |
R1465 |
T2389 |
T2366 |
punct |
.,is |
R1466 |
T2391 |
T2392 |
amod |
TMPred,analysis |
R1467 |
T2392 |
T2393 |
nsubj |
analysis,predicts |
R1468 |
T2393 |
T2394 |
ccomp |
predicts,diverge |
R1469 |
T2395 |
T2396 |
mark |
that,encodes |
R1470 |
T2396 |
T2393 |
ccomp |
encodes,predicts |
R1471 |
T2397 |
T2396 |
nsubj |
isoform,encodes |
R1472 |
T2398 |
T2397 |
nummod |
2,isoform |
R1473 |
T2399 |
T2400 |
det |
a,protein |
R1474 |
T2400 |
T2396 |
dobj |
protein,encodes |
R1475 |
T2401 |
T2400 |
amod |
identical,protein |
R1476 |
T2402 |
T2401 |
prep |
in,identical |
R1477 |
T2403 |
T2402 |
pobj |
structure,in |
R1478 |
T2404 |
T2401 |
prep |
to,identical |
R1479 |
T2405 |
T2404 |
pobj |
Mcoln1,to |
R1480 |
T2406 |
T2400 |
punct |
", ",protein |
R1481 |
T2407 |
T2400 |
acl |
possessing,protein |
R1482 |
T2408 |
T2409 |
nummod |
6,domains |
R1483 |
T2409 |
T2407 |
dobj |
domains,possessing |
R1484 |
T2410 |
T2409 |
compound |
transmembrane,domains |
R1485 |
T2411 |
T2409 |
cc |
and,domains |
R1486 |
T2412 |
T2413 |
det |
a,pore |
R1487 |
T2413 |
T2409 |
conj |
pore,domains |
R1488 |
T2414 |
T2413 |
compound |
channel,pore |
R1489 |
T2415 |
T2394 |
punct |
", ",diverge |
R1490 |
T2416 |
T2394 |
advmod |
however,diverge |
R1491 |
T2417 |
T2418 |
det |
the,sequences |
R1492 |
T2418 |
T2394 |
nsubj |
sequences,diverge |
R1493 |
T2419 |
T2418 |
compound |
protein,sequences |
R1494 |
T2420 |
T2394 |
prep |
at,diverge |
R1495 |
T2421 |
T2422 |
amod |
amino,acid |
R1496 |
T2422 |
T2420 |
pobj |
acid,at |
R1497 |
T2423 |
T2422 |
nummod |
526,acid |
R1498 |
T2424 |
T2394 |
punct |
.,diverge |
R1499 |
T2426 |
T2427 |
det |
The,tail |
R1500 |
T2427 |
T2435 |
nsubj |
tail,is |
R1501 |
T2428 |
T2429 |
nummod |
55,acid |
R1502 |
T2429 |
T2427 |
nmod |
acid,tail |
R1503 |
T2430 |
T2429 |
nmod |
amino,acid |
R1504 |
T2431 |
T2432 |
npadvmod |
C,terminal |
R1505 |
T2432 |
T2427 |
amod |
terminal,tail |
R1506 |
T2433 |
T2432 |
punct |
-,terminal |
R1507 |
T2434 |
T2427 |
amod |
cytoplasmic,tail |
R1508 |
T2436 |
T2427 |
acl |
encoded,tail |
R1509 |
T2437 |
T2436 |
agent |
by,encoded |
R1510 |
T2438 |
T2439 |
det |
the,transcript |
R1511 |
T2439 |
T2437 |
pobj |
transcript,by |
R1512 |
T2440 |
T2441 |
nummod |
2.4,kb |
R1513 |
T2441 |
T2439 |
compound |
kb,transcript |
R1514 |
T2442 |
T2443 |
advmod |
completely,different |
R1515 |
T2443 |
T2435 |
acomp |
different,is |
R1516 |
T2444 |
T2443 |
prep |
from,different |
R1517 |
T2445 |
T2446 |
det |
the,tail |
R1518 |
T2446 |
T2444 |
pobj |
tail,from |
R1519 |
T2447 |
T2448 |
nummod |
86,acid |
R1520 |
T2448 |
T2446 |
compound |
acid,tail |
R1521 |
T2449 |
T2448 |
compound |
amino,acid |
R1522 |
T2450 |
T2446 |
acl |
encoded,tail |
R1523 |
T2451 |
T2450 |
agent |
by,encoded |
R1524 |
T2452 |
T2453 |
det |
the,transcript |
R1525 |
T2453 |
T2451 |
pobj |
transcript,by |
R1526 |
T2454 |
T2455 |
npadvmod |
murine,specific |
R1527 |
T2455 |
T2453 |
amod |
specific,transcript |
R1528 |
T2456 |
T2457 |
nummod |
4.4,kb |
R1529 |
T2457 |
T2453 |
compound |
kb,transcript |
R1530 |
T2458 |
T2459 |
punct |
(,Fig. |
R1531 |
T2459 |
T2435 |
parataxis |
Fig.,is |
R1532 |
T2460 |
T2459 |
nummod |
4,Fig. |
R1533 |
T2461 |
T2459 |
punct |
),Fig. |
R1534 |
T2462 |
T2435 |
punct |
.,is |
R1535 |
T2464 |
T2465 |
compound |
Clontech,Blots |
R1536 |
T2465 |
T2469 |
nsubjpass |
Blots,hybridized |
R1537 |
T2466 |
T2467 |
compound |
Mouse,RNA |
R1538 |
T2467 |
T2465 |
compound |
RNA,Blots |
R1539 |
T2468 |
T2465 |
compound |
Master,Blots |
R1540 |
T2469 |
T2471 |
ccomp |
hybridized,was |
R1541 |
T2470 |
T2469 |
auxpass |
were,hybridized |
R1542 |
T2472 |
T2469 |
prep |
with,hybridized |
R1543 |
T2473 |
T2474 |
det |
the,probes |
R1544 |
T2474 |
T2472 |
pobj |
probes,with |
R1545 |
T2475 |
T2474 |
nmod |
exon,probes |
R1546 |
T2476 |
T2475 |
nummod |
2,exon |
R1547 |
T2477 |
T2475 |
cc |
and,exon |
R1548 |
T2478 |
T2475 |
conj |
intron,exon |
R1549 |
T2479 |
T2478 |
nummod |
12,intron |
R1579 |
T2509 |
T2471 |
parataxis |
shown,was |
R1580 |
T2510 |
T2509 |
nsubj |
data,shown |
R1581 |
T2511 |
T2509 |
neg |
not,shown |
R1582 |
T2512 |
T2509 |
punct |
),shown |
R1583 |
T2513 |
T2471 |
punct |
.,was |
R1584 |
T2515 |
T2516 |
advmod |
Next,compared |
R1585 |
T2517 |
T2516 |
punct |
", ",compared |
R1586 |
T2518 |
T2516 |
nsubj |
we,compared |
R1587 |
T2519 |
T2516 |
advmod |
directly,compared |
R1588 |
T2520 |
T2521 |
det |
the,sequence |
R1589 |
T2521 |
T2516 |
dobj |
sequence,compared |
R1590 |
T2522 |
T2521 |
nmod |
nucleotide,sequence |
R1591 |
T2523 |
T2522 |
cc |
and,nucleotide |
R1592 |
T2524 |
T2525 |
compound |
amino,acid |
R1593 |
T2525 |
T2522 |
conj |
acid,nucleotide |
R1594 |
T2526 |
T2521 |
prep |
of,sequence |
R1595 |
T2527 |
T2528 |
det |
the,transcript |
R1596 |
T2528 |
T2526 |
pobj |
transcript,of |
R1597 |
T2529 |
T2530 |
advmod |
alternatively,spliced |
R1598 |
T2530 |
T2528 |
amod |
spliced,transcript |
R1599 |
T2531 |
T2528 |
compound |
mouse,transcript |
R1600 |
T2532 |
T2516 |
prep |
to,compared |
R1601 |
T2533 |
T2534 |
det |
the,sequence |
R1602 |
T2534 |
T2532 |
pobj |
sequence,to |
R1603 |
T2535 |
T2534 |
amod |
entire,sequence |
R1604 |
T2536 |
T2537 |
amod |
human,MCOLN1 |
R1605 |
T2537 |
T2534 |
nmod |
MCOLN1,sequence |
R1606 |
T2538 |
T2534 |
amod |
genomic,sequence |
R1607 |
T2539 |
T2516 |
cc |
and,compared |
R1608 |
T2540 |
T2516 |
conj |
found,compared |
R1609 |
T2541 |
T2542 |
det |
no,similarity |
R1610 |
T2542 |
T2540 |
dobj |
similarity,found |
R1611 |
T2543 |
T2542 |
amod |
significant,similarity |
R1612 |
T2544 |
T2516 |
punct |
.,compared |
R1613 |
T2546 |
T2547 |
mark |
As,mentioned |
R1614 |
T2547 |
T2548 |
advcl |
mentioned,show |
R1615 |
T2549 |
T2547 |
advmod |
previously,mentioned |
R1616 |
T2550 |
T2548 |
punct |
", ",show |
R1617 |
T2551 |
T2552 |
compound |
Northern,blots |
R1618 |
T2552 |
T2548 |
nsubj |
blots,show |
R1619 |
T2553 |
T2552 |
acl |
performed,blots |
R1620 |
T2554 |
T2553 |
prep |
with,performed |
R1621 |
T2555 |
T2556 |
amod |
human,MCOLN1 |
R1622 |
T2556 |
T2557 |
compound |
MCOLN1,probes |
R1623 |
T2557 |
T2554 |
pobj |
probes,with |
R1624 |
T2558 |
T2559 |
advmod |
only,transcript |
R1625 |
T2559 |
T2548 |
dobj |
transcript,show |
R1626 |
T2560 |
T2559 |
nummod |
one,transcript |
R1627 |
T2561 |
T2562 |
nummod |
2.4,kb |
R1628 |
T2562 |
T2559 |
compound |
kb,transcript |
R1629 |
T2563 |
T2548 |
punct |
.,show |
R1630 |
T2565 |
T2566 |
prep |
In,hybridized |
R1631 |
T2567 |
T2565 |
pobj |
addition,In |
R1632 |
T2568 |
T2566 |
punct |
", ",hybridized |
R1633 |
T2569 |
T2566 |
nsubj |
we,hybridized |
R1634 |
T2570 |
T2571 |
det |
a,Northern |
R1635 |
T2571 |
T2566 |
dobj |
Northern,hybridized |
R1636 |
T2572 |
T2571 |
amod |
human,Northern |
R1637 |
T2573 |
T2574 |
amod |
multiple,tissue |
R1638 |
T2574 |
T2571 |
compound |
tissue,Northern |
R1639 |
T2575 |
T2571 |
cc |
and,Northern |
R1640 |
T2576 |
T2577 |
amod |
human,Southern |
R1641 |
T2577 |
T2571 |
conj |
Southern,Northern |
R1642 |
T2578 |
T2566 |
prep |
with,hybridized |
R1643 |
T2579 |
T2580 |
det |
a,probe |
R1644 |
T2580 |
T2578 |
pobj |
probe,with |
R1645 |
T2581 |
T2580 |
prep |
in,probe |
R1646 |
T2582 |
T2583 |
amod |
human,intron |
R1647 |
T2583 |
T2581 |
pobj |
intron,in |
R1648 |
T2584 |
T2583 |
nummod |
12,intron |
R1649 |
T2585 |
T2586 |
dep |
that,is |
R1650 |
T2586 |
T2580 |
relcl |
is,probe |
R1651 |
T2587 |
T2586 |
acomp |
adjacent,is |
R1652 |
T2588 |
T2587 |
prep |
to,adjacent |
R1653 |
T2589 |
T2588 |
pobj |
exon,to |
R1654 |
T2590 |
T2589 |
nummod |
13,exon |
R1655 |
T2591 |
T2566 |
punct |
.,hybridized |
R1656 |
T2593 |
T2594 |
det |
The,probe |
R1657 |
T2594 |
T2595 |
nsubjpass |
probe,located |
R1658 |
T2596 |
T2595 |
auxpass |
was,located |
R1659 |
T2597 |
T2595 |
prep |
in,located |
R1660 |
T2598 |
T2599 |
det |
the,region |
R1661 |
T2599 |
T2597 |
pobj |
region,in |
R1662 |
T2600 |
T2599 |
amod |
syntenic,region |
R1663 |
T2601 |
T2600 |
prep |
to,syntenic |
R1664 |
T2602 |
T2601 |
pobj |
that,to |
R1665 |
T2603 |
T2604 |
dep |
which,encodes |
R1666 |
T2604 |
T2602 |
relcl |
encodes,that |
R1667 |
T2605 |
T2606 |
det |
the,transcript |
R1668 |
T2606 |
T2604 |
dobj |
transcript,encodes |
R1669 |
T2607 |
T2606 |
amod |
alternate,transcript |
R1670 |
T2608 |
T2606 |
compound |
mouse,transcript |
R1671 |
T2609 |
T2595 |
punct |
.,located |
R1672 |
T2611 |
T2612 |
advmod |
Only,bands |
R1673 |
T2612 |
T2615 |
nsubjpass |
bands,detected |
R1674 |
T2613 |
T2612 |
det |
the,bands |
R1675 |
T2614 |
T2612 |
amod |
expected,bands |
R1676 |
T2616 |
T2615 |
auxpass |
were,detected |
R1677 |
T2617 |
T2615 |
prep |
on,detected |
R1678 |
T2618 |
T2619 |
det |
the,Southern |
R1679 |
T2619 |
T2617 |
pobj |
Southern,on |
R1680 |
T2620 |
T2615 |
cc |
and,detected |
R1681 |
T2621 |
T2622 |
det |
no,bands |
R1682 |
T2622 |
T2623 |
nsubjpass |
bands,detected |
R1683 |
T2623 |
T2615 |
conj |
detected,detected |
R1684 |
T2624 |
T2623 |
auxpass |
were,detected |
R1685 |
T2625 |
T2623 |
prep |
on,detected |
R1686 |
T2626 |
T2627 |
det |
the,Northern |
R1687 |
T2627 |
T2625 |
pobj |
Northern,on |
R1688 |
T2628 |
T2615 |
punct |
", ",detected |
R1689 |
T2629 |
T2615 |
advcl |
confirming,detected |
R1690 |
T2630 |
T2631 |
mark |
that,is |
R1691 |
T2631 |
T2629 |
ccomp |
is,confirming |
R1692 |
T2632 |
T2633 |
det |
this,transcript |
R1693 |
T2633 |
T2631 |
nsubj |
transcript,is |
R1694 |
T2634 |
T2633 |
amod |
alternative,transcript |
R1695 |
T2635 |
T2631 |
acomp |
specific,is |
R1696 |
T2636 |
T2635 |
prep |
to,specific |
R1697 |
T2637 |
T2638 |
amod |
murine,Mcoln1 |
R1698 |
T2638 |
T2636 |
pobj |
Mcoln1,to |
R1699 |
T2639 |
T2615 |
punct |
.,detected |
R1700 |
T2641 |
T2642 |
amod |
Recent,analysis |
R1701 |
T2642 |
T2644 |
nsubj |
analysis,yields |
R1702 |
T2643 |
T2642 |
compound |
BLASTP,analysis |
R1703 |
T2645 |
T2642 |
prep |
of,analysis |
R1704 |
T2646 |
T2647 |
det |
the,transcript |
R1705 |
T2647 |
T2645 |
pobj |
transcript,of |
R1706 |
T2648 |
T2647 |
amod |
alternate,transcript |
R1707 |
T2649 |
T2647 |
compound |
Mcoln1,transcript |
R1708 |
T2650 |
T2651 |
det |
a,match |
R1709 |
T2651 |
T2644 |
dobj |
match,yields |
R1710 |
T2652 |
T2651 |
prep |
to,match |
R1711 |
T2653 |
T2654 |
det |
a,protein |
R1712 |
T2654 |
T2652 |
pobj |
protein,to |
R1713 |
T2655 |
T2654 |
amod |
putative,protein |
R1714 |
T2656 |
T2657 |
nummod |
145,acid |
R1715 |
T2657 |
T2654 |
nmod |
acid,protein |
R1716 |
T2658 |
T2657 |
nmod |
amino,acid |
R1717 |
T2659 |
T2654 |
amod |
anonymous,protein |
R1718 |
T2660 |
T2661 |
punct |
(,BAB25862 |
R1719 |
T2661 |
T2654 |
parataxis |
BAB25862,protein |
R1720 |
T2662 |
T2661 |
compound |
GB,BAB25862 |
R1721 |
T2663 |
T2661 |
compound |
No.,BAB25862 |
R1722 |
T2664 |
T2661 |
punct |
),BAB25862 |
R1723 |
T2665 |
T2654 |
acl |
predicted,protein |
R1724 |
T2666 |
T2665 |
prep |
from,predicted |
R1725 |
T2667 |
T2668 |
det |
a,clone |
R1726 |
T2668 |
T2666 |
pobj |
clone,from |
R1727 |
T2669 |
T2668 |
compound |
RIKEN,clone |
R1728 |
T2670 |
T2644 |
punct |
.,yields |
R1729 |
T2672 |
T2673 |
nsubj |
It,is |
R1730 |
T2674 |
T2673 |
acomp |
obvious,is |
R1731 |
T2675 |
T2673 |
prep |
from,is |
R1732 |
T2676 |
T2677 |
poss |
our,results |
R1733 |
T2677 |
T2675 |
pobj |
results,from |
R1734 |
T2678 |
T2673 |
punct |
", ",is |
R1735 |
T2679 |
T2673 |
advmod |
however,is |
R1736 |
T2680 |
T2673 |
punct |
", ",is |
R1737 |
T2681 |
T2682 |
mark |
that,is |
R1738 |
T2682 |
T2673 |
ccomp |
is,is |
R1739 |
T2683 |
T2684 |
det |
the,identification |
R1740 |
T2684 |
T2682 |
nsubj |
identification,is |
R1741 |
T2685 |
T2684 |
prep |
of,identification |
R1742 |
T2686 |
T2687 |
det |
this,sequence |
R1743 |
T2687 |
T2685 |
pobj |
sequence,of |
R1744 |
T2688 |
T2684 |
prep |
as,identification |
R1745 |
T2689 |
T2690 |
det |
a,protein |
R1746 |
T2690 |
T2688 |
pobj |
protein,as |
R1747 |
T2691 |
T2692 |
amod |
full,length |
R1748 |
T2692 |
T2690 |
compound |
length,protein |
R1749 |
T2693 |
T2692 |
punct |
-,length |
R1750 |
T2694 |
T2682 |
acomp |
incorrect,is |
R1751 |
T2695 |
T2696 |
mark |
since,identify |
R1753 |
T2697 |
T2696 |
nsubj |
probes,identify |
R1754 |
T2698 |
T2697 |
amod |
unique,probes |
R1755 |
T2699 |
T2698 |
prep |
to,unique |
R1756 |
T2700 |
T2701 |
det |
the,clone |
R1757 |
T2701 |
T2699 |
pobj |
clone,to |
R1758 |
T2702 |
T2697 |
punct |
", ",probes |
R1759 |
T2703 |
T2704 |
advmod |
as,as |
R1760 |
T2704 |
T2697 |
cc |
as,probes |
R1761 |
T2705 |
T2704 |
advmod |
well,as |
R1762 |
T2706 |
T2697 |
conj |
probes,probes |
R1763 |
T2707 |
T2706 |
acl |
containing,probes |
R1764 |
T2708 |
T2709 |
det |
the,sequence |
R2884 |
T4631 |
T4632 |
compound |
Peptide,sequence |
R2885 |
T4632 |
T4633 |
compound |
sequence,comparison |
R2886 |
T4634 |
T4633 |
prep |
of,comparison |
R2887 |
T4635 |
T4636 |
det |
the,isoforms |
R2888 |
T4636 |
T4634 |
pobj |
isoforms,of |
R2889 |
T4637 |
T4636 |
nummod |
two,isoforms |
R2890 |
T4638 |
T4639 |
advmod |
alternatively,spliced |
R2891 |
T4639 |
T4636 |
amod |
spliced,isoforms |
R2892 |
T4640 |
T4636 |
compound |
Mcoln1,isoforms |
R2893 |
T4641 |
T4633 |
punct |
.,comparison |
R2894 |
T4643 |
T4644 |
det |
The,box |
R2895 |
T4644 |
T4646 |
nsubj |
box,surrounds |
R2896 |
T4645 |
T4644 |
amod |
green,box |
R2897 |
T4647 |
T4648 |
det |
the,tails |
R2898 |
T4648 |
T4646 |
dobj |
tails,surrounds |
R2899 |
T4649 |
T4648 |
amod |
divergent,tails |
R2900 |
T4650 |
T4651 |
npadvmod |
c,terminal |
R2901 |
T4651 |
T4648 |
amod |
terminal,tails |
R2902 |
T4652 |
T4651 |
punct |
-,terminal |
R2903 |
T4653 |
T4648 |
amod |
cytoplasmic,tails |
R2904 |
T4654 |
T4646 |
punct |
.,surrounds |
R2905 |
T4656 |
T4657 |
det |
The,lines |
R2906 |
T4657 |
T4659 |
nsubj |
lines,indicate |
R2907 |
T4658 |
T4657 |
amod |
blue,lines |
R2908 |
T4660 |
T4661 |
det |
the,domains |
R2909 |
T4661 |
T4659 |
dobj |
domains,indicate |
R2910 |
T4662 |
T4661 |
compound |
transmembrane,domains |
R2911 |
T4663 |
T4659 |
punct |
.,indicate |
R1772 |
T2716 |
T2673 |
punct |
.,is |
R1550 |
T2480 |
T2474 |
acl |
mentioned,probes |
R1551 |
T2481 |
T2480 |
advmod |
above,mentioned |
R1552 |
T2482 |
T2469 |
prep |
in,hybridized |
R1553 |
T2483 |
T2484 |
det |
an,attempt |
R1554 |
T2484 |
T2482 |
pobj |
attempt,in |
R1555 |
T2485 |
T2486 |
aux |
to,determine |
R1556 |
T2486 |
T2484 |
acl |
determine,attempt |
R1557 |
T2487 |
T2488 |
mark |
if,showed |
R1558 |
T2488 |
T2486 |
ccomp |
showed,determine |
R1559 |
T2489 |
T2490 |
det |
these,transcripts |
R1560 |
T2490 |
T2488 |
nsubj |
transcripts,showed |
R1561 |
T2491 |
T2490 |
nummod |
two,transcripts |
R1562 |
T2492 |
T2488 |
dobj |
differences,showed |
R1563 |
T2493 |
T2492 |
prep |
in,differences |
R1564 |
T2494 |
T2495 |
compound |
expression,patterns |
R1565 |
T2495 |
T2493 |
pobj |
patterns,in |
R1566 |
T2496 |
T2471 |
punct |
", ",was |
R1567 |
T2497 |
T2471 |
advmod |
however,was |
R1568 |
T2498 |
T2471 |
punct |
", ",was |
R1569 |
T2499 |
T2471 |
expl |
there,was |
R1570 |
T2500 |
T2501 |
det |
no,difference |
R1571 |
T2501 |
T2471 |
attr |
difference,was |
R1572 |
T2502 |
T2501 |
amod |
significant,difference |
R1573 |
T2503 |
T2471 |
prep |
in,was |
R1574 |
T2504 |
T2505 |
det |
the,tissues |
R1575 |
T2505 |
T2503 |
pobj |
tissues,in |
R1576 |
T2506 |
T2505 |
nummod |
22,tissues |
R1577 |
T2507 |
T2505 |
acl |
represented,tissues |
R1578 |
T2508 |
T2509 |
punct |
(,shown |
R1752 |
T2696 |
T2682 |
advcl |
identify,is |
R1765 |
T2709 |
T2707 |
dobj |
sequence,containing |
R1766 |
T2710 |
T2709 |
compound |
Mcoln1,sequence |
R1767 |
T2711 |
T2709 |
compound |
coding,sequence |
R1768 |
T2712 |
T2696 |
punct |
", ",identify |
R1769 |
T2713 |
T2714 |
det |
the,transcripts |
R1770 |
T2714 |
T2696 |
dobj |
transcripts,identify |
R1771 |
T2715 |
T2714 |
amod |
same,transcripts |