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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T4154","span":{"begin":23,"end":28},"obj":"NCBITaxon:10088"},{"id":"T4155","span":{"begin":29,"end":35},"obj":"UBERON:0000922"},{"id":"T4156","span":{"begin":45,"end":51},"obj":"UBERON:0000479"},{"id":"T4157","span":{"begin":70,"end":75},"obj":"NCBITaxon:10088"},{"id":"T4158","span":{"begin":76,"end":81},"obj":"UBERON:0007023"},{"id":"T4159","span":{"begin":91,"end":97},"obj":"UBERON:0000479"},{"id":"T4160","span":{"begin":196,"end":206},"obj":"GO:0097617"},{"id":"T4161","span":{"begin":216,"end":219},"obj":"CHEBI:37972"},{"id":"T4162","span":{"begin":220,"end":224},"obj":"CHEBI_EXT:dATP"},{"id":"T4163","span":{"begin":225,"end":232},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T4164","span":{"begin":233,"end":236},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T4165","span":{"begin":237,"end":248},"obj":"_FRAGMENT"},{"id":"T4166","span":{"begin":256,"end":269},"obj":"SO_EXT:0001384"},{"id":"T4167","span":{"begin":249,"end":255},"obj":"PR_EXT:000010252"},{"id":"T4168","span":{"begin":287,"end":291},"obj":"SO_EXT:0000147"},{"id":"T4169","span":{"begin":327,"end":337},"obj":"SO_EXT:1001187"},{"id":"T4170","span":{"begin":341,"end":346},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T4171","span":{"begin":383,"end":388},"obj":"SO_EXT:0000147"},{"id":"T4172","span":{"begin":404,"end":411},"obj":"SO_EXT:0000112"},{"id":"T4173","span":{"begin":438,"end":445},"obj":"SO:0001030"},{"id":"T4174","span":{"begin":479,"end":486},"obj":"SO:0001031"},{"id":"T4175","span":{"begin":496,"end":505},"obj":"GO:0097617"},{"id":"T4176","span":{"begin":549,"end":559},"obj":"GO:0097617"},{"id":"T4177","span":{"begin":565,"end":572},"obj":"PR_EXT:000003676"},{"id":"T4178","span":{"begin":573,"end":579},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T4179","span":{"begin":581,"end":595},"obj":"GO:0097617"},{"id":"T4180","span":{"begin":681,"end":686},"obj":"CHEMINF_GO_EXT:chemical_binding_or_bond_formation"},{"id":"T4181","span":{"begin":687,"end":693},"obj":"CHEBI_SO_EXT:molecular_probe"}],"relations":[{"id":"R2611","pred":"_lexicallyChainedTo","subj":"T4166","obj":"T4165"}],"text":"Northern Blot Analysis\nMouse embryo multiple-tissue northern blot and mouse adult multiple-tissue northern blot filters were purchased from Clontech Laboratories (Palo Alto, CA). The filters were hybridized with the 32P-dATP labeled DNA fragment of Mcoln1 coding region corresponding to exon 2 (see above). For the alternative transcript, a probe was generated in the region between exons 12 and 13 with primers 5'-GTGTCCACCACCTGAGAG-3' (forward) and 5'-GAAGTAGCATTCCTGCAGGC-3' (reverse) with an annealing temperature of 62°C. The filters were then hybridized with β-actin probes. Hybridizations and washes were carried out in standard conditions, with the stripping of previously bound probes in between [16]."}