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PMC:88885 / 15009-15548 JSONTXT

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craft-sa-dev

Id Subject Object Predicate Lexical cue
T3839 0-5 JJ denotes Total
T3840 20-23 NN denotes DNA
T3841 6-11 NN denotes mouse
T3842 12-19 JJ denotes genomic
T3843 28-36 VBN denotes digested
T3844 24-27 VBD denotes was
T3845 37-42 VBG denotes using
T3846 43-48 NN denotes EcoRI
T3847 48-50 , denotes ,
T3848 50-55 NN denotes BamHI
T3849 55-57 , denotes ,
T3850 57-61 NN denotes PstI
T3851 61-63 , denotes ,
T3852 63-66 CC denotes and
T3853 67-71 NN denotes XbaI
T3854 71-72 . denotes .
T3855 72-239 sentence denotes The digests were electrophoresed on a 1% agarose gel at 60V overnight and were transferred onto a Hybond N+ membrane from Amersham Pharmacia Biotech (Piscataway, NJ).
T3856 73-76 DT denotes The
T3857 77-84 NNS denotes digests
T3858 90-105 VBN denotes electrophoresed
T3859 85-89 VBD denotes were
T3860 106-108 IN denotes on
T3861 109-110 DT denotes a
T3862 122-125 NN denotes gel
T3863 111-112 CD denotes 1
T3864 112-113 NN denotes %
T3865 114-121 NN denotes agarose
T3866 126-128 IN denotes at
T3867 129-132 NN denotes 60V
T3868 133-142 RB denotes overnight
T3869 143-146 CC denotes and
T3870 147-151 VBD denotes were
T3871 152-163 VBN denotes transferred
T3872 164-168 IN denotes onto
T3873 169-170 DT denotes a
T3874 181-189 NN denotes membrane
T3875 171-177 NN denotes Hybond
T3876 178-180 JJ denotes N+
T3877 190-194 IN denotes from
T3878 195-203 NNP denotes Amersham
T3879 214-221 NNP denotes Biotech
T3880 204-213 NNP denotes Pharmacia
T3881 222-223 -LRB- denotes (
T3882 223-233 NNP denotes Piscataway
T3883 233-235 , denotes ,
T3884 235-237 NNP denotes NJ
T3885 237-238 -RRB- denotes )
T3886 238-239 . denotes .
T3887 239-468 sentence denotes A 32P-dATP labeled PCR fragment of the Mcoln1 coding region corresponding to exon 2 was used as a probe (primers 5'-CCCCACAGAAGAGGAAGAC-3' (forward) and 5'-AGATCTTGACCACCTGCAG-3' (reverse) with an annealing temperature of 59°C).
T3888 240-241 DT denotes A
T3889 263-271 NN denotes fragment
T3890 242-245 NN denotes 32P
T3891 246-250 NN denotes dATP
T3892 245-246 HYPH denotes -
T3893 251-258 JJ denotes labeled
T3894 259-262 NN denotes PCR
T3895 328-332 VBN denotes used
T3896 272-274 IN denotes of
T3897 275-278 DT denotes the
T3898 293-299 NN denotes region
T3899 279-285 NN denotes Mcoln1
T3900 286-292 NN denotes coding
T3901 300-313 VBG denotes corresponding
T3902 314-316 IN denotes to
T3903 317-321 NN denotes exon
T3904 322-323 CD denotes 2
T3905 324-327 VBD denotes was
T3906 333-335 IN denotes as
T3907 336-337 DT denotes a
T3908 338-343 NN denotes probe
T3909 344-345 -LRB- denotes (
T3910 376-377 NN denotes 3
T3911 345-352 NNS denotes primers
T3912 353-354 CD denotes 5
T3913 354-355 SYM denotes '
T3914 355-356 HYPH denotes -
T3915 356-375 NN denotes CCCCACAGAAGAGGAAGAC
T3916 375-376 HYPH denotes -
T3917 377-378 SYM denotes '
T3918 379-380 -LRB- denotes (
T3919 380-387 RB denotes forward
T3920 387-388 -RRB- denotes )
T3921 389-392 CC denotes and
T3922 393-394 CD denotes 5
T3923 416-417 NN denotes 3
T3924 394-395 SYM denotes '
T3925 395-396 HYPH denotes -
T3926 396-415 NN denotes AGATCTTGACCACCTGCAG
T3927 415-416 HYPH denotes -
T3928 417-418 SYM denotes '
T3929 419-420 -LRB- denotes (
T3930 420-427 RB denotes reverse
T3931 427-428 -RRB- denotes )
T3932 429-433 IN denotes with
T3933 434-436 DT denotes an
T3934 447-458 NN denotes temperature
T3935 437-446 JJ denotes annealing
T3936 459-461 IN denotes of
T3937 462-464 CD denotes 59
T3938 464-466 NNS denotes °C
T3939 466-467 -RRB- denotes )
T3940 467-468 . denotes .
T3941 468-539 sentence denotes Hybridization and washes were carried out in standard conditions [16].
T3942 469-482 NN denotes Hybridization
T3943 499-506 VBN denotes carried
T3944 483-486 CC denotes and
T3945 487-493 NNS denotes washes
T3946 494-498 VBD denotes were
T3947 507-510 RP denotes out
T3948 511-513 IN denotes in
T3949 514-522 JJ denotes standard
T3950 523-533 NNS denotes conditions
T3951 534-535 -LRB- denotes [
T3952 535-537 CD denotes 16
T3953 537-538 -RRB- denotes ]
T3954 538-539 . denotes .
R2368 T3839 T3840 amod Total,DNA
R2369 T3840 T3843 nsubjpass DNA,digested
R2370 T3841 T3840 nmod mouse,DNA
R2371 T3842 T3840 amod genomic,DNA
R2372 T3844 T3843 auxpass was,digested
R2373 T3845 T3843 advcl using,digested
R2374 T3846 T3845 dobj EcoRI,using
R2375 T3847 T3846 punct ", ",EcoRI
R2376 T3848 T3846 conj BamHI,EcoRI
R2377 T3849 T3848 punct ", ",BamHI
R2378 T3850 T3848 conj PstI,BamHI
R2379 T3851 T3850 punct ", ",PstI
R2380 T3852 T3850 cc and,PstI
R2381 T3853 T3850 conj XbaI,PstI
R2382 T3854 T3843 punct .,digested
R2383 T3856 T3857 det The,digests
R2384 T3857 T3858 nsubjpass digests,electrophoresed
R2385 T3859 T3858 auxpass were,electrophoresed
R2386 T3860 T3858 prep on,electrophoresed
R2387 T3861 T3862 det a,gel
R2388 T3862 T3860 pobj gel,on
R2389 T3863 T3864 nummod 1,%
R2390 T3864 T3862 compound %,gel
R2391 T3865 T3862 compound agarose,gel
R2392 T3866 T3858 prep at,electrophoresed
R2393 T3867 T3866 pobj 60V,at
R2394 T3868 T3858 advmod overnight,electrophoresed
R2395 T3869 T3858 cc and,electrophoresed
R2396 T3870 T3871 auxpass were,transferred
R2397 T3871 T3858 conj transferred,electrophoresed
R2398 T3872 T3871 prep onto,transferred
R2399 T3873 T3874 det a,membrane
R2400 T3874 T3872 pobj membrane,onto
R2401 T3875 T3874 nmod Hybond,membrane
R2402 T3876 T3875 amod N+,Hybond
R2403 T3877 T3871 prep from,transferred
R2404 T3878 T3879 compound Amersham,Biotech
R2405 T3879 T3877 pobj Biotech,from
R2406 T3880 T3879 compound Pharmacia,Biotech
R2407 T3881 T3882 punct (,Piscataway
R2408 T3882 T3879 parataxis Piscataway,Biotech
R2409 T3883 T3882 punct ", ",Piscataway
R2410 T3884 T3882 npadvmod NJ,Piscataway
R2411 T3885 T3882 punct ),Piscataway
R2412 T3886 T3858 punct .,electrophoresed
R2413 T3888 T3889 det A,fragment
R2414 T3889 T3895 nsubjpass fragment,used
R2415 T3890 T3891 compound 32P,dATP
R2416 T3891 T3893 npadvmod dATP,labeled
R2417 T3892 T3891 punct -,dATP
R2418 T3893 T3889 amod labeled,fragment
R2419 T3894 T3889 compound PCR,fragment
R2420 T3896 T3889 prep of,fragment
R2421 T3897 T3898 det the,region
R2422 T3898 T3896 pobj region,of
R2423 T3899 T3898 compound Mcoln1,region
R2424 T3900 T3898 compound coding,region
R2425 T3901 T3898 acl corresponding,region
R2426 T3902 T3901 prep to,corresponding
R2427 T3903 T3902 pobj exon,to
R2428 T3904 T3903 nummod 2,exon
R2429 T3905 T3895 auxpass was,used
R2430 T3906 T3895 prep as,used
R2431 T3907 T3908 det a,probe
R2432 T3908 T3906 pobj probe,as
R2433 T3909 T3910 punct (,3
R2434 T3910 T3895 parataxis 3,used
R2435 T3911 T3910 nmod primers,3
R2436 T3912 T3910 nummod 5,3
R2437 T3913 T3912 punct ',5
R2438 T3914 T3910 punct -,3
R2439 T3915 T3910 compound CCCCACAGAAGAGGAAGAC,3
R2440 T3916 T3910 punct -,3
R2441 T3917 T3910 punct ',3
R2442 T3918 T3919 punct (,forward
R2443 T3919 T3910 parataxis forward,3
R2444 T3920 T3919 punct ),forward
R2445 T3921 T3910 cc and,3
R2446 T3922 T3923 nummod 5,3
R2447 T3923 T3910 conj 3,3
R2448 T3924 T3922 punct ',5
R2449 T3925 T3923 punct -,3
R2450 T3926 T3923 compound AGATCTTGACCACCTGCAG,3
R2451 T3927 T3923 punct -,3
R2452 T3928 T3923 punct ',3
R2453 T3929 T3930 punct (,reverse
R2454 T3930 T3923 parataxis reverse,3
R2455 T3931 T3930 punct ),reverse
R2456 T3932 T3910 prep with,3
R2457 T3933 T3934 det an,temperature
R2458 T3934 T3932 pobj temperature,with
R2459 T3935 T3934 amod annealing,temperature
R2460 T3936 T3934 prep of,temperature
R2461 T3937 T3938 nummod 59,°C
R2462 T3938 T3936 pobj °C,of
R2463 T3939 T3910 punct ),3
R2464 T3940 T3895 punct .,used
R2465 T3942 T3943 nsubjpass Hybridization,carried
R2466 T3944 T3942 cc and,Hybridization
R2467 T3945 T3942 conj washes,Hybridization
R2468 T3946 T3943 auxpass were,carried
R2469 T3947 T3943 prt out,carried
R2470 T3948 T3943 prep in,carried
R2471 T3949 T3950 amod standard,conditions
R2472 T3950 T3948 pobj conditions,in
R2473 T3951 T3952 punct [,16
R2474 T3952 T3943 parataxis 16,carried
R2475 T3953 T3952 punct ],16
R2476 T3954 T3943 punct .,carried

craft-ca-core-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T3821 6-11 NCBITaxon:10088 denotes mouse
T3822 12-19 SO:0001026 denotes genomic
T3823 50-55 PR:P23940 denotes BamHI
T3824 114-121 CHEBI:2511 denotes agarose
T3825 242-245 CHEBI:37972 denotes 32P
T3827 263-274,286-299 SO:0001384 denotes fragment of coding region
T3828 279-285 PR:000010252 denotes Mcoln1
T3829 317-321 SO:0000147 denotes exon
T3830 345-352 SO:0000112 denotes primers
T3831 380-387 SO:0001030 denotes forward
T3832 420-427 SO:0001031 denotes reverse
T3833 437-446 GO:0097617 denotes annealing
T3834 469-482 GO:0097617 denotes Hybridization

craft-ca-core-ex-dev

Below, discontinuous spans are shown in the bag model. You can change it to the chain model.

Id Subject Object Predicate Lexical cue
T3955 6-11 NCBITaxon:10088 denotes mouse
T3956 12-23 SO_EXT:genomic_DNA denotes genomic DNA
T3957 20-23 CHEBI_SO_EXT:DNA denotes DNA
T3958 50-55 PR_EXT:P23940 denotes BamHI
T3959 114-121 CHEBI:2511 denotes agarose
T3960 152-163 GO_EXT:biological_movement_or_translocation_process denotes transferred
T3961 242-245 CHEBI:37972 denotes 32P
T3962 246-250 CHEBI_EXT:dATP denotes dATP
T3963 251-258 CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process denotes labeled
T3965 263-274,286-299 SO_EXT:0001384 denotes fragment of coding region
T3966 279-285 PR_EXT:000010252 denotes Mcoln1
T3967 317-321 SO_EXT:0000147 denotes exon
T3968 338-343 CHEBI_SO_EXT:molecular_probe denotes probe
T3969 345-352 SO_EXT:0000112 denotes primers
T3970 380-387 SO:0001030 denotes forward
T3971 420-427 SO:0001031 denotes reverse
T3972 437-446 GO:0097617 denotes annealing
T3973 469-482 GO:0097617 denotes Hybridization