PMC:7796291 / 23066-24595 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"228","span":{"begin":47,"end":51},"obj":"Gene"},{"id":"229","span":{"begin":1354,"end":1358},"obj":"Chemical"},{"id":"230","span":{"begin":1360,"end":1364},"obj":"Chemical"},{"id":"231","span":{"begin":1370,"end":1373},"obj":"Chemical"}],"attributes":[{"id":"A228","pred":"tao:has_database_id","subj":"228","obj":"Gene:13897326"},{"id":"A229","pred":"tao:has_database_id","subj":"229","obj":"MESH:D006997"},{"id":"A230","pred":"tao:has_database_id","subj":"230","obj":"MESH:D000431"},{"id":"A231","pred":"tao:has_database_id","subj":"231","obj":"MESH:D019840"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Bactericidal or bacteria removal efficiency of Resp. A is shown in Figure 6 and Figure S7. In the 2.5 cm × 2.5 cm designated area of each web, non-treated samples showed ~1.46 × 106 and ~2.53 × 106 CFUs on the coverweb and stiffener, respectively. Microwaving, oven-dry, and UV treatment showed similar bactericidal effect up to about 82%; in fact, this is inconsistent with previous studies [18,20], where 99.99% inactivation efficiency was achieved. This discrepancy can be attributed to the different treatment conditions, such as irradiation intensity, treatment time, and amount of bacteria loaded. In a previous study, 1.0 × 106 CFU of bacteria was loaded on the respirator with a nebulizer; and the UV irradiation (wavelength; 254 nm, 5 min) and microwaving (400 W, 10 min) resulted in complete inactivation of cells [18]. The case of an oven-dry treatment (100 °C, 15 min) also achieved 99.99% inactivation efficiency, when 5.0 × 104 CFU of bacteria was dropped onto the surface of the respirator [20]. Compared to previous studies, this study built a harsher experimental condition with a higher loading of bacteria (5.0 × 106 CFU). As a result, the intensity of microwaving (750 W, 2 min), UV irradiation (wavelength; 253.7 nm, 2 h), and oven-dry (90 °C, 1 h) of this study were insufficient for complete sterilization [51]. Chemical solvents (ClO−, EtOH, and IPA) and laundering treatments showed no color change with cell staining, indicating 100% bactericidal or bacteria removal efficiency (Figure 6 and Figure S7)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T187","span":{"begin":0,"end":52},"obj":"Sentence"},{"id":"T188","span":{"begin":53,"end":90},"obj":"Sentence"},{"id":"T189","span":{"begin":91,"end":247},"obj":"Sentence"},{"id":"T190","span":{"begin":248,"end":451},"obj":"Sentence"},{"id":"T191","span":{"begin":452,"end":603},"obj":"Sentence"},{"id":"T192","span":{"begin":604,"end":829},"obj":"Sentence"},{"id":"T193","span":{"begin":830,"end":1010},"obj":"Sentence"},{"id":"T194","span":{"begin":1011,"end":1141},"obj":"Sentence"},{"id":"T195","span":{"begin":1142,"end":1334},"obj":"Sentence"},{"id":"T196","span":{"begin":1335,"end":1529},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Bactericidal or bacteria removal efficiency of Resp. A is shown in Figure 6 and Figure S7. In the 2.5 cm × 2.5 cm designated area of each web, non-treated samples showed ~1.46 × 106 and ~2.53 × 106 CFUs on the coverweb and stiffener, respectively. Microwaving, oven-dry, and UV treatment showed similar bactericidal effect up to about 82%; in fact, this is inconsistent with previous studies [18,20], where 99.99% inactivation efficiency was achieved. This discrepancy can be attributed to the different treatment conditions, such as irradiation intensity, treatment time, and amount of bacteria loaded. In a previous study, 1.0 × 106 CFU of bacteria was loaded on the respirator with a nebulizer; and the UV irradiation (wavelength; 254 nm, 5 min) and microwaving (400 W, 10 min) resulted in complete inactivation of cells [18]. The case of an oven-dry treatment (100 °C, 15 min) also achieved 99.99% inactivation efficiency, when 5.0 × 104 CFU of bacteria was dropped onto the surface of the respirator [20]. Compared to previous studies, this study built a harsher experimental condition with a higher loading of bacteria (5.0 × 106 CFU). As a result, the intensity of microwaving (750 W, 2 min), UV irradiation (wavelength; 253.7 nm, 2 h), and oven-dry (90 °C, 1 h) of this study were insufficient for complete sterilization [51]. Chemical solvents (ClO−, EtOH, and IPA) and laundering treatments showed no color change with cell staining, indicating 100% bactericidal or bacteria removal efficiency (Figure 6 and Figure S7)."}