> top > docs > PMC:7796052 > spans > 13955-15331 > annotations

PMC:7796052 / 13955-15331 JSONTXT

Annnotations TAB JSON ListView MergeView

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
356 362-372 Gene denotes bradykinin Gene:3827
357 656-666 Gene denotes bradykinin Gene:3827
358 546-548 Gene denotes BK Gene:3827
359 512-514 Gene denotes BK Gene:3827
360 321-323 Gene denotes BK Gene:3827
361 127-132 Species denotes human Tax:9606
362 56-66 Chemical denotes Raloxifene MESH:D020849
363 307-309 Chemical denotes 3H MESH:D014316
364 358-360 Chemical denotes 3H MESH:D014316
365 506-510 Chemical denotes Leu8
366 580-590 Chemical denotes raloxifene MESH:D020849
367 652-654 Chemical denotes 3H MESH:D014316
368 707-717 Chemical denotes raloxifene MESH:D020849
369 203-206 CellLine denotes CHO CVCL:0213
379 888-899 Gene denotes B2 receptor Gene:624
380 1120-1131 Gene denotes B2 receptor Gene:624
381 1249-1251 Gene denotes BK Gene:3827
382 1328-1330 Gene denotes BK Gene:3827
383 1114-1119 Species denotes human Tax:9606
384 1027-1031 Chemical denotes Ca2+ MESH:D000069285
385 1218-1222 Chemical denotes Ca2+ MESH:D000069285
386 1357-1361 Chemical denotes Ca2+ MESH:D000069285
387 1081-1084 CellLine denotes CHO CVCL:0213

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T91 0-4 Sentence denotes 3.2.
T92 5-20 Sentence denotes In Vitro Assays
T93 22-28 Sentence denotes 3.2.1.
T94 29-55 Sentence denotes Ligand Displacement Assays
T95 56-222 Sentence denotes Raloxifene was tested for its ability to displace reference ligands on human recombinant bradykinin B1 or B2 receptors, respectively, expressed in CHO cells at 20 µM.
T96 223-431 Sentence denotes For this purpose, first saturation isotherms were obtained with reference ligands ([3H] des-Arg10-BK (0.35 nM) in the case of B1, and [3H]-bradykinin (0.2 nM) for B2) incubated for 60 min at room temperature.
T97 432-565 Sentence denotes Nonspecific binding was evaluated by adding a reference compound (desArg9[Leu8]-BK at 10 µM in the case of B1 and BK at 1 µM for B2).
T98 566-798 Sentence denotes Antagonism of raloxifene was measured as a percent inhibition of specific binding of [3H]-bradykinin as control, obtained in the presence of raloxifene at 20 µM and using as reference compounds desArg10-KD for B1 and NPC-567 for B2.
T99 800-806 Sentence denotes 3.2.2.
T100 807-833 Sentence denotes Functional Efficacy Assays
T101 834-964 Sentence denotes Measurementent of the efficacy of the compound on the B2 receptor was carried out following the protocol described elsewhere [31].
T102 965-1147 Sentence denotes The method is based on measuring differences in intracellular Ca2+ concentrations produced in diverse conditions on CHO cells expressing recombinant human B2 receptor by fluorometry.
T103 1148-1268 Sentence denotes Agonism was measured through the capacity of the compound to increase Ca2+ concentration compared to BK (EC50 ~ 2.4 pM).
T104 1269-1376 Sentence denotes Antagonism was measured through the capacity to antagonize BK based on the reduction of Ca2+ concentration.