| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T58 |
0-219 |
Sentence |
denotes |
The uncharged PAS moiety, which does not alter the isoelectric point of the target peptide, facilitates classical protein precipitation by ammonium sulfate, thus providing an efficient and inexpensive purification step. |
| T59 |
220-464 |
Sentence |
denotes |
After adjusting the cleared whole cell extract prepared by mechanical cell lysis to 30% ammonium sulfate saturation, most of the host cell proteins remained in solution while both PAS-Tα1 and PAS-Tα1 were selectively recovered as a precipitate. |
| T60 |
465-631 |
Sentence |
denotes |
To remove residual E. coli proteins, the redissolved precipitate was subjected to ion exchange chromatography on a salt-tolerant anion exchange (AEX) resin at pH 8.5. |
| T61 |
632-899 |
Sentence |
denotes |
Even though the PASylated Tα1 peptide with a calculated pI of 4.3 [38] for both versions should be negatively charged under these conditions and, hence, is expected to adsorb to the resin, the recombinant fusion proteins were quantitatively found in the flow-through. |
| T62 |
900-1028 |
Sentence |
denotes |
Possibly, the voluminous PAS polymer partially shields the small peptide from ionic interactions with the chromatography matrix. |
| T63 |
1029-1149 |
Sentence |
denotes |
Nevertheless, this step resulted in efficient depletion both of residual host cell proteins and of bacterial endotoxins. |
