PMC:7795856 / 7597-7990 JSONTXT

{"project":"LitCovid-PubTator","denotations":[{"id":"183","span":{"begin":60,"end":63},"obj":"Gene"},{"id":"184","span":{"begin":168,"end":171},"obj":"Gene"},{"id":"186","span":{"begin":162,"end":167},"obj":"Species"},{"id":"187","span":{"begin":294,"end":301},"obj":"Species"}],"attributes":[{"id":"A183","pred":"tao:has_database_id","subj":"183","obj":"Gene:134864"},{"id":"A184","pred":"tao:has_database_id","subj":"184","obj":"Gene:134864"},{"id":"A186","pred":"tao:has_database_id","subj":"186","obj":"Tax:9606"},{"id":"A187","pred":"tao:has_database_id","subj":"187","obj":"Tax:562"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"To achieve C-terminal PASylation of N-terminally acetylated Tα1, a plasmid harboring a bicistronic operon was constructed to allow the simultaneous expression of human Tα1 (UniProtKB ID: P06454; residues 2–29), C-terminally fused with a PAS polypeptide comprising 601 amino acids [34], and the E. coli N-acetyltransferase RimJ (UniProtKB ID: P0A948) based on the vector pASK75 (Figure 1) [36]."}

{"project":"LitCovid-sentences","denotations":[{"id":"T50","span":{"begin":0,"end":393},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"To achieve C-terminal PASylation of N-terminally acetylated Tα1, a plasmid harboring a bicistronic operon was constructed to allow the simultaneous expression of human Tα1 (UniProtKB ID: P06454; residues 2–29), C-terminally fused with a PAS polypeptide comprising 601 amino acids [34], and the E. coli N-acetyltransferase RimJ (UniProtKB ID: P0A948) based on the vector pASK75 (Figure 1) [36]."}