
PMC:7795856 / 39525-40528
Annnotations
LitCovid-PubTator
Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
---|---|---|---|---|---|
844 | 56-59 | Gene | denotes | Tα1 | Gene:134864 |
845 | 190-193 | Gene | denotes | Tα1 | Gene:134864 |
846 | 365-368 | Gene | denotes | Tα1 | Gene:134864 |
847 | 443-446 | Gene | denotes | Tα1 | Gene:134864 |
848 | 608-611 | Gene | denotes | Tα1 | Gene:134864 |
849 | 734-737 | Gene | denotes | Tα1 | Gene:134864 |
850 | 778-781 | Gene | denotes | Tα1 | Gene:134864 |
851 | 359-364 | Species | denotes | human | Tax:9606 |
852 | 465-472 | Species | denotes | E. coli | Tax:562 |
853 | 323-333 | Chemical | denotes | methionine | MESH:D008715 |
854 | 340-347 | Chemical | denotes | proline | MESH:D011392 |
855 | 641-651 | Chemical | denotes | methionine | MESH:D008715 |
LitCovid-sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T301 | 195-848 | Sentence | denotes | The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity > 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL. |
T302 | 849-1003 | Sentence | denotes | Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa. |