PMC:7795856 / 39525-40528 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
844	56-59	Gene	denotes	Tα1	Gene:134864
845	190-193	Gene	denotes	Tα1	Gene:134864
846	365-368	Gene	denotes	Tα1	Gene:134864
847	443-446	Gene	denotes	Tα1	Gene:134864
848	608-611	Gene	denotes	Tα1	Gene:134864
849	734-737	Gene	denotes	Tα1	Gene:134864
850	778-781	Gene	denotes	Tα1	Gene:134864
851	359-364	Species	denotes	human	Tax:9606
852	465-472	Species	denotes	E. coli	Tax:562
853	323-333	Chemical	denotes	methionine	MESH:D008715
854	340-347	Chemical	denotes	proline	MESH:D011392
855	641-651	Chemical	denotes	methionine	MESH:D008715

Id	Subject	Object	Predicate	Lexical cue
T301	195-848	Sentence	denotes	The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity > 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL.
T302	849-1003	Sentence	denotes	Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa.