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PMC:7795856 / 39525-40528 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
844 56-59 Gene denotes Tα1 Gene:134864
845 190-193 Gene denotes Tα1 Gene:134864
846 365-368 Gene denotes Tα1 Gene:134864
847 443-446 Gene denotes Tα1 Gene:134864
848 608-611 Gene denotes Tα1 Gene:134864
849 734-737 Gene denotes Tα1 Gene:134864
850 778-781 Gene denotes Tα1 Gene:134864
851 359-364 Species denotes human Tax:9606
852 465-472 Species denotes E. coli Tax:562
853 323-333 Chemical denotes methionine MESH:D008715
854 340-347 Chemical denotes proline MESH:D011392
855 641-651 Chemical denotes methionine MESH:D008715

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T301 195-848 Sentence denotes The gene cassette cloned on pASK75 comprises the T7 RBS, the coding region for a 601-residue PAS#1 sequence preceded by a start methionine and a proline codon, the human Tα1 sequence, and, finally, tlpp. (b) The deconvoluted ESI-MS spectrum of PAS-Tα1 purified from the E. coli cell extract by subtractive AEX and CEX (see text) reveals a molecular mass of 52,789.69 Da, which matches the calculated mass for PAS-Tα1 with a fully processed start methionine (52,789.66 Da). (c) Reverse-phase chromatography indicates a purity > 96% for PAS-Tα1. (d) Analytical SEC of the purified PAS-Tα1 in PBS results in a single peak with an elution volume of 13.2 mL.
T302 849-1003 Sentence denotes Comparison with a half-logarithmic calibration curve (inset) indicates a strongly expanded hydrodynamic volume with an apparent molecular size of 665 kDa.