PMC:7795856 / 38587-39514 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
826	12-15	Gene	denotes	Tα1	Gene:134864
827	120-123	Gene	denotes	Tα1	Gene:134864
828	662-665	Gene	denotes	Tα1	Gene:134864
829	251-261	Chemical	denotes	acetylated
830	597-617	Chemical	denotes	N-acetylated Tα1-PAS
831	689-714	Chemical	denotes	phosphate-buffered saline

Id	Subject	Object	Predicate	Lexical cue
T298	283-780	Sentence	denotes	52,734.56 Da). (b) ESI-MS analysis of the bound and eluted peptide fraction from the same chromatography reveals a molecular mass of 52,692.37 Da, which matches the calculated mass of non-acetylated Tα1-PAS (52,692.54 Da). (c) Reverse-phase chromatography of the flow-through fraction indicates a purity > 96% for N-acetylated Tα1-PAS. (d) Analytical SEC of the finally purified Tα1-PAS in the presence of phosphate-buffered saline (PBS) results in a single peak with an elution volume of 13.5 mL.
T299	781-927	Sentence	denotes	Comparison with a half-logarithmic calibration curve (inset) indicates an expanded hydrodynamic volume with an apparent molecular size of 557 kDa.