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PMC:7795856 / 37648-38576 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
812 330-333 Gene denotes Tα1 Gene:134864
813 324-329 Species denotes human Tax:9606
814 523-530 Species denotes E. coli Tax:562
815 34-65 Chemical denotes N-terminally acetylated Tα1-PAS
816 191-195 Chemical denotes tetp
817 496-499 Chemical denotes SDS MESH:D012967
818 732-735 Chemical denotes SDS MESH:D012967
819 810-826 Chemical denotes ammonium sulfate MESH:D000645

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T293 0-198 Sentence denotes Figure 1 Bacterial production of N-terminally acetylated Tα1-PAS. (a) Expression vector for Tα1-PAS and the N-acetyltransferase RimJ from a bicistronic operon under control of the inducible tetp/o.
T294 199-603 Sentence denotes The gene cassette cloned on pASK75 comprises the T7 phage ribosome binding site (T7 RBS) followed by the coding sequence for human Tα1 fused to a 601-residue PAS#1 sequence, the second RBS, the coding sequence for RimJ, and the lipoprotein terminator (tlpp). (b) Western blot analysis after 4–12% SDS–PAGE of Tα1-PAS in the E. coli whole cell lysate using an antibody directed against the PAS#1 sequence.
T295 604-761 Sentence denotes M, protein size standard; lane 1, whole cell extract before induction; lane 2, whole cell extract 15 h after induction. (c) 10% SDS-PAGE of purified Tα1-PAS.
T296 762-928 Sentence denotes M, protein size standard; lane 1, Tα1-PAS after ammonium sulfate precipitation, subtractive anion exchange (AEX), cation exchange (CEX), and final AEX chromatography.