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PMC:7795856 / 32775-34206 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
580 248-261 Gene denotes serum albumin Gene:213
581 613-616 Gene denotes Tα1 Gene:50719
582 764-767 Gene denotes Tα1 Gene:50719
583 889-909 Gene denotes alkaline phosphatase Gene:16870
584 935-955 Gene denotes alkaline phosphatase Gene:16870
585 1332-1335 Gene denotes Tα1 Gene:50719
586 344-347 Species denotes rat Tax:10116
587 503-506 Species denotes rat Tax:10116
588 639-642 Species denotes rat Tax:10116
589 1358-1361 Species denotes rat Tax:10116
590 1104-1127 Chemical denotes p-nitrophenyl phosphate MESH:C008644
591 823-829 Disease denotes mL PBS MESH:D011535

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T199 0-5 Sentence denotes 4.10.
T200 6-43 Sentence denotes ELISA Quantification of PASylated Tα1
T201 44-164 Sentence denotes A 96-well Nunc Maxisorb ELISA plate (ThermoFisher) was coated with 100 µg/mL anti-PAS antibody in PBS at 4 °C overnight.
T202 165-305 Sentence denotes After washing twice with PBS/T, free binding sites were blocked with 3% w/v bovine serum albumin (BSA) in PBS/T at room temperature for 1 h.
T203 306-528 Sentence denotes After washing 3 times with PBS/T, the rat plasma samples were applied in dilution series in PBS/T supplemented with 0.5% (v/v) plasma from an untreated animal (to maintain a constant proportion of rat plasma constituents).
T204 529-677 Sentence denotes In the same manner, a standard curve was prepared using dilution series of purified Tα1-PAS (used for spiking rat plasma) at defined concentrations.
T205 678-747 Sentence denotes After incubation for 1 h at RT, wells were washed 3 times with PBS/T.
T206 748-1010 Sentence denotes To detect bound Tα1-PAS, wells were incubated for 1 h with 50 µl of a 1 µg/mL PBS/T solution of the second anti-PAS antibody conjugated with alkaline phosphatase using the Lightning-Link alkaline phosphatase antibody labeling kit (BioTechne, Wiesbaden, Germany).
T207 1011-1140 Sentence denotes After washing twice with PBS/T and twice with PBS, the enzymatic activity was detected using p-nitrophenyl phosphate (0.5 mg/mL).
T208 1141-1431 Sentence denotes To this end, the plate was incubated for 20 min at 30 °C, the absorbance was measured at 405 nm using a SpectraMax M5e microtiter plate reader (Molecular Devices, Sunnyvale, CA, USA) and the Tα1-PAS concentrations in rat plasma samples were quantified by comparison with the standard curve.