PMC:7795856 / 31844-32773 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
563	54-57	Gene	denotes	Tα1	Gene:134864
564	239-242	Gene	denotes	Tα1	Gene:134864
565	354-357	Gene	denotes	Tα1	Gene:134864
566	268-273	Chemical	denotes	water	MESH:D014867
567	321-326	Chemical	denotes	water	MESH:D014867

Id	Subject	Object	Predicate	Lexical cue
T192	0-90	Sentence	denotes	Due to the absence of aromatic side chains, PASylated Tα1 does not absorb light at 280 nm.
T193	91-213	Sentence	denotes	Thus, for reliable quantification via the peptide backbone absorption, an extinction coefficient at 225 nm was determined.
T194	214-327	Sentence	denotes	To this end, highly pure Tα1-PAS was lyophilized from water, weighed, and dissolved in a defined volume of water.
T195	328-562	Sentence	denotes	The concentration of this Tα1-PAS solution was additionally quantified by measuring the backbone peptide groups using UV absorption at 205 nm using a generic calculated extinction coefficient [75], which led to a fair agreement (±6%).
T196	563-716	Sentence	denotes	The absorbance at 225 nm was measured for various dilutions of the gravimetrically prepared peptide stock solution and plotted against the concentration.
T197	717-824	Sentence	denotes	The linear range was fitted by a straight line, resulting in an extinction coefficient of 253,057 M−1⋅cm−1.
T198	825-929	Sentence	denotes	While most buffers strongly absorb light at 205 nm, such a spectral overlap is usually absent at 225 nm.