Id |
Subject |
Object |
Predicate |
Lexical cue |
T192 |
0-90 |
Sentence |
denotes |
Due to the absence of aromatic side chains, PASylated Tα1 does not absorb light at 280 nm. |
T193 |
91-213 |
Sentence |
denotes |
Thus, for reliable quantification via the peptide backbone absorption, an extinction coefficient at 225 nm was determined. |
T194 |
214-327 |
Sentence |
denotes |
To this end, highly pure Tα1-PAS was lyophilized from water, weighed, and dissolved in a defined volume of water. |
T195 |
328-562 |
Sentence |
denotes |
The concentration of this Tα1-PAS solution was additionally quantified by measuring the backbone peptide groups using UV absorption at 205 nm using a generic calculated extinction coefficient [75], which led to a fair agreement (±6%). |
T196 |
563-716 |
Sentence |
denotes |
The absorbance at 225 nm was measured for various dilutions of the gravimetrically prepared peptide stock solution and plotted against the concentration. |
T197 |
717-824 |
Sentence |
denotes |
The linear range was fitted by a straight line, resulting in an extinction coefficient of 253,057 M−1⋅cm−1. |
T198 |
825-929 |
Sentence |
denotes |
While most buffers strongly absorb light at 205 nm, such a spectral overlap is usually absent at 225 nm. |