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PMC:7795856 / 31804-32773 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
557 32-35 Gene denotes Tα1 Gene:134864
563 94-97 Gene denotes Tα1 Gene:134864
564 279-282 Gene denotes Tα1 Gene:134864
565 394-397 Gene denotes Tα1 Gene:134864
566 308-313 Chemical denotes water MESH:D014867
567 361-366 Chemical denotes water MESH:D014867

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T190 0-4 Sentence denotes 4.9.
T191 5-39 Sentence denotes Quantification of Purified Tα1-PAS
T192 40-130 Sentence denotes Due to the absence of aromatic side chains, PASylated Tα1 does not absorb light at 280 nm.
T193 131-253 Sentence denotes Thus, for reliable quantification via the peptide backbone absorption, an extinction coefficient at 225 nm was determined.
T194 254-367 Sentence denotes To this end, highly pure Tα1-PAS was lyophilized from water, weighed, and dissolved in a defined volume of water.
T195 368-602 Sentence denotes The concentration of this Tα1-PAS solution was additionally quantified by measuring the backbone peptide groups using UV absorption at 205 nm using a generic calculated extinction coefficient [75], which led to a fair agreement (±6%).
T196 603-756 Sentence denotes The absorbance at 225 nm was measured for various dilutions of the gravimetrically prepared peptide stock solution and plotted against the concentration.
T197 757-864 Sentence denotes The linear range was fitted by a straight line, resulting in an extinction coefficient of 253,057 M−1⋅cm−1.
T198 865-969 Sentence denotes While most buffers strongly absorb light at 205 nm, such a spectral overlap is usually absent at 225 nm.