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PMC:7795856 / 25823-28012 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
465 825-828 Gene denotes Tα1 Gene:134864
466 840-843 Gene denotes Tα1 Gene:134864
467 1015-1018 Gene denotes Tα1 Gene:134864
468 1269-1272 Gene denotes Tα1 Gene:134864
469 1538-1541 Gene denotes Tα1 Gene:134864
470 1648-1651 Gene denotes Tα1 Gene:134864
471 82-93 Chemical denotes citric acid MESH:D019343
472 357-373 Chemical denotes ammonium sulfate MESH:D000645
473 512-522 Chemical denotes AEX buffer
474 524-528 Chemical denotes sAEX
475 536-544 Chemical denotes Tris/HCl
476 551-555 Chemical denotes EDTA MESH:D004492
477 633-637 Chemical denotes sAEX
478 960-971 Chemical denotes sAEX buffer
479 1085-1095 Chemical denotes CEX buffer
480 1103-1113 Chemical denotes Na-citrate
481 1182-1199 Chemical denotes TOYOPEARL Sulfate
482 1253-1256 Chemical denotes CEX
483 1348-1352 Chemical denotes NaCl MESH:D012965
484 1604-1608 Chemical denotes NaCl MESH:D012965
485 1714-1724 Chemical denotes AEX buffer
486 1732-1744 Chemical denotes ethanolamine MESH:D019856
487 1745-1748 Chemical denotes HCl MESH:D006851
488 1893-1903 Chemical denotes AEX buffer
489 1966-1970 Chemical denotes NaCl MESH:D012965
490 2070-2078 Chemical denotes peptides MESH:D010455
491 2130-2135 Chemical denotes water MESH:D014867
492 103-116 Disease denotes EmulsiFlex C5 MESH:C537005
493 1783-1805 Disease denotes mL Fractogel® EMD TMAE MESH:C562593

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T150 0-163 Sentence denotes Bacteria were harvested by centrifugation and disrupted in the presence of 100 mM citric acid using an EmulsiFlex C5 cell homogenizer (Avestin, Mannheim, Germany).
T151 164-319 Sentence denotes After centrifugation for 20 min (39,200× g) at 16 °C, the supernatant was filter-sterilized using a 0.45 µm syringe filter (Sartorius, Göttingen, Germany).
T152 320-464 Sentence denotes The clear filtrate was adjusted with ammonium sulfate to 30% saturation, stirred for 30 min at RT, and centrifuged for 30 min (39,200× g) at RT.
T153 465-653 Sentence denotes The precipitate was solubilized in subtractive AEX buffer (sAEX; 20 mM Tris/HCl, 1 mM EDTA, pH 8.5), filter-sterilized, and dialyzed twice against a 100-fold volume of sAEX buffer at 4 °C.
T154 654-803 Sentence denotes Subsequent chromatography steps were performed on an ÄKTA Explorer 100 system (GE Healthcare, Freiburg, Germany) operated at a flow rate of 5 mL/min.
T155 804-972 Sentence denotes The dialyzed sample (Tα1-PAS or PAS-Tα1) was first applied to a 5 mL TOYOPEARL NH2-750F column (Tosoh Bioscience, Griesheim, Germany) equilibrated with the sAEX buffer.
T156 973-1131 Sentence denotes The flow-through containing the PASylated Tα1 was collected and dialyzed twice against a 100-fold volume of the CEX buffer (20 mM Na-citrate, pH 3.0) at 4 °C.
T157 1132-1264 Sentence denotes The protein solution was then applied to an 85 mL TOYOPEARL Sulfate-650F column (Tosoh Bioscience) equilibrated with the CEX buffer.
T158 1265-1409 Sentence denotes PAS-Tα1 quantitatively bound to the column and the pure protein was eluted using a NaCl concentration gradient (0–250 mM over 2 column volumes).
T159 1410-1632 Sentence denotes In the case of Tα1-PAS, the CEX flow-through fraction contained the N-terminally acetylated protein, whereas the non-acetylated Tα1-PAS stayed bound to the column and could again be eluted in a NaCl concentration gradient.
T160 1633-1904 Sentence denotes The acetylated Tα1-PAS fraction from the flow-through was dialyzed twice against AEX buffer (20 mM ethanolamine/HCl, pH 10) and then applied to a 100 mL Fractogel® EMD TMAE (M)strong anion exchanger (Merck Millipore, Burlington, MA, USA) equilibrated with the AEX buffer.
T161 1905-2045 Sentence denotes This time, highly pure acetylated Tα1-PAS was eluted using a NaCl concentration gradient (0–200 mM over 2 column volumes) in the AEX buffer.
T162 2046-2189 Sentence denotes Both purified PASylated peptides were dialyzed 5 times against a 200-fold volume of water, lyophilized, and stored at −20 °C until further use.