PMC:7795856 / 14971-15851 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue	tao:has_database_id
269	156-163	Gene	denotes	insulin	Gene:3630
270	286-289	Gene	denotes	Tα1	Gene:134864
271	843-846	Gene	denotes	Tα1	Gene:134864
272	466-469	Chemical	denotes	PAS
273	523-527	Chemical	denotes	RimJ

Id	Subject	Object	Predicate	Lexical cue
T92	0-426	Sentence	denotes	Of note, in contrast to classical approaches, which involve the use of an insoluble fusion partner, for example, an α-galactosidase fragment in the case of insulin [43], to provoke formation of inclusion bodies and protect the gene product from intracellular proteolysis, the PASylated Tα1 peptide was recovered as a soluble fusion protein, thus allowing direct purification from the cell extract without solubilization steps.
T93	427-551	Sentence	denotes	Furthermore, the C-terminally attached PAS moiety was compatible with N-terminal acetylation by RimJ as confirmed by ESI-MS.
T94	552-880	Sentence	denotes	Yields of our expression study at the research scale reached 15 mg purified acetylated Tα1-PAS and even 50 mg PAS-Tα1 per 1 L bacterial culture (at an optical density (OD) of 3), which surpasses the reported yield of a bacterially produced (yet probably non-acetylated and non-glycosylated) Tα1-Fc fusion protein (16 mg/L) [44].