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PMC:7795856 / 11731-13125 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
234 582-585 Gene denotes Tα1 Gene:134864
235 923-926 Gene denotes Tα1 Gene:134864
236 982-985 Gene denotes Tα1 Gene:134864
237 15-22 Chemical denotes peptide MESH:D010455
238 131-138 Chemical denotes Tα1-PAS
239 458-476 Chemical denotes acetylated Tα1-PAS
240 1202-1210 Chemical denotes peptides MESH:D010455
241 842-853 Disease denotes aggregation MESH:D001791

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T70 0-126 Sentence denotes Both PASylated peptide preparations had a purity > 96% as indicated by reverse-phase chromatography (Figure 2c and Figure 3c).
T71 127-229 Sentence denotes For Tα1-PAS, we performed a final AEX polishing step, which also allowed concentration of the peptide.
T72 230-356 Sentence denotes At pH 10, the acetylated Tα1-PAS bound to a strong AEX resin and eluted as a homogenous peak in a salt concentration gradient.
T73 357-503 Sentence denotes The endotoxin content of this fraction was very low, with < 0.1 EU/mg, and the final yield was 15 mg acetylated Tα1-PAS per 1 L bacterial culture.
T74 504-705 Sentence denotes In comparison, the final yield of the fully column-bound (non-acetylated) PAS-Tα1 reached 50 mg per 1 L bacterial culture after CEX chromatography and, again, the endotoxin content was below 0.1 EU/mg.
T75 706-894 Sentence denotes Analytical size exclusion chromatography (SEC) of both PASylated peptide versions revealed a single symmetric peak without any signs of aggregation or truncation (Figure 2d and Figure 3d).
T76 895-961 Sentence denotes The N-terminally acetylated Tα1-PAS eluted at 13.5 mL (bed volume:
T77 962-1083 Sentence denotes 24 mL), whereas PAS-Tα1 eluted at 13.2 mL, thus indicating apparent molecular sizes of 557 kDa and 665 kDa, respectively.
T78 1084-1394 Sentence denotes This is more than 10 times (Tα1-PAS) or even 12 times (PAS-Tα1) larger than the true molecular mass of both PASylated peptides (52.7 kDa), which demonstrates the huge expansion of the hydrodynamic molecular volume caused by the random-coil structure of the PAS polymer, in line with previous observations [35].