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PMC:7795856 / 115-1524 JSONTXT

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LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
18 13-16 Gene denotes Tα1 Gene:134864
19 533-536 Gene denotes Tα1 Gene:134864
20 71-88 Species denotes hepatitis B virus Tax:10407
21 540-556 Species denotes Escherichia coli Tax:562
22 903-907 Species denotes rats Tax:10116
23 966-975 Species denotes synthetic Tax:2005392
24 90-93 Species denotes HBV Tax:10407
25 475-476 Chemical denotes N MESH:D009584
26 492-496 Chemical denotes RimJ
27 810-813 Chemical denotes Pro MESH:D011392
28 814-817 Chemical denotes Ala MESH:D000409
29 818-821 Chemical denotes Ser MESH:D012694
30 823-826 Chemical denotes PAS
31 99-133 Disease denotes hepatitis C virus (HCV) infections MESH:D006526
32 212-220 Disease denotes COVID-19 MESH:C000657245
33 221-231 Disease denotes infections MESH:D007239
34 236-242 Disease denotes cancer MESH:D009369
35 991-1006 Disease denotes retarded kidney MESH:D007674

LitCovid-PD-HP

Id Subject Object Predicate Lexical cue hp_id
T2 71-80 Phenotype denotes hepatitis http://purl.obolibrary.org/obo/HP_0012115
T3 99-108 Phenotype denotes hepatitis http://purl.obolibrary.org/obo/HP_0012115
T4 236-242 Phenotype denotes cancer http://purl.obolibrary.org/obo/HP_0002664

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T4 0-243 Sentence denotes Thymosin α1 (Tα1) is an immunostimulatory peptide for the treatment of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections and used as an immune enhancer, which also offers prospects in the context of COVID-19 infections and cancer.
T5 244-407 Sentence denotes Manufacturing of this N-terminally acetylated 28-residue peptide is demanding, and its short plasma half-life limits in vivo efficacy and requires frequent dosing.
T6 408-571 Sentence denotes Here, we combined the PASylation technology with enzymatic in situ N-acetylation by RimJ to produce a long-acting version of Tα1 in Escherichia coli at high yield.
T7 572-687 Sentence denotes ESI-MS analysis of the purified fusion protein indicated the expected composition without any signs of proteolysis.
T8 688-856 Sentence denotes SEC analysis revealed a 10-fold expanded hydrodynamic volume resulting from the fusion with a conformationally disordered Pro/Ala/Ser (PAS) polypeptide of 600 residues.
T9 857-1018 Sentence denotes This size effect led to a plasma half-life in rats extended by more than a factor 8 compared to the original synthetic peptide due to retarded kidney filtration.
T10 1019-1136 Sentence denotes Our study provides the basis for therapeutic development of a next generation thymosin α1 with prolonged circulation.
T11 1137-1409 Sentence denotes Generally, the strategy of producing an N-terminally protected PASylated peptide solves three major problems of peptide drugs: (i) instability in the expression host, (ii) rapid degradation by serum exopeptidases, and (iii) low bioactivity because of fast renal clearance.