|
article-title
|
MicroRNA-633 enhances melanoma cell proliferation and migration by suppressing KAI1
|
|
abstract
|
The aim of the present study was to determine the impact of microRNA (miRNA/miR)-633 on the biological properties of malignant melanoma cells. Kang-Ai 1 (KAI1), also known as cluster of differentiation 82, is an important transcriptional regulator and tumor suppressor gene present in different types of tumors. miRNAs that potentially bind with KAI1 were predicted via bioinformatics analyses. In total, six putative miRNA regulators of KAI1 were identified in the present analysis, among which miR-633 was upregulated the most in melanoma tissues compared with the control group. The expression levels of miR-633 and KAI1 in melanoma tissues compared with adjacent normal tissues were then assessed. It was found that miR-633 was significantly upregulated in melanoma cells compared with the control group, whereas the expression levels of KAI1 showed the opposite results. miR-633 was predicted to target the 3′-untranslated region of KAI1 using predictive online tools, and results from luciferase reporter assays confirmed the direct regulation of KAI1 promoter activity by miR-633. Furthermore, miR-633 mimics over expression was shown to suppress both mRNA and protein expression of KAI1, while miR-633 inhibition resulted in decreased viability and migrationin melanoma cells in vitro. Taken together, the present study demonstrated, to the best of the authors' knowledge for the first time, that miR-633 exerts an important role in melanoma through targeting KAI1.
|
|
p
|
The aim of the present study was to determine the impact of microRNA (miRNA/miR)-633 on the biological properties of malignant melanoma cells. Kang-Ai 1 (KAI1), also known as cluster of differentiation 82, is an important transcriptional regulator and tumor suppressor gene present in different types of tumors. miRNAs that potentially bind with KAI1 were predicted via bioinformatics analyses. In total, six putative miRNA regulators of KAI1 were identified in the present analysis, among which miR-633 was upregulated the most in melanoma tissues compared with the control group. The expression levels of miR-633 and KAI1 in melanoma tissues compared with adjacent normal tissues were then assessed. It was found that miR-633 was significantly upregulated in melanoma cells compared with the control group, whereas the expression levels of KAI1 showed the opposite results. miR-633 was predicted to target the 3′-untranslated region of KAI1 using predictive online tools, and results from luciferase reporter assays confirmed the direct regulation of KAI1 promoter activity by miR-633. Furthermore, miR-633 mimics over expression was shown to suppress both mRNA and protein expression of KAI1, while miR-633 inhibition resulted in decreased viability and migrationin melanoma cells in vitro. Taken together, the present study demonstrated, to the best of the authors' knowledge for the first time, that miR-633 exerts an important role in melanoma through targeting KAI1.
|
|
body
|
Introduction
Melanoma is one of the most commonly occurring forms of cancer, and malignant melanoma is the third most common skin malignancy (1–6). For most patients with melanoma, immunotherapy, chemotherapy or small-molecule inhibitor administration are not effective therapies (7). Furthermore, the outcomes of patients with advanced-stage disease remain poor (8,9).
MicroRNAs (miRNAs/miRs) are short RNAs (~22 nucleotides in length) that do not encode protein, and yet are important regulators of oncogenesis (10,11). By binding to complementary sequences in the 3′-untranslated region (3′-UTR) of target mRNAs, miRNAs can alter the stability and translation of these transcripts, thereby influencing phenotypic outcomes within cells. A single miRNA can target multiple different mRNAs, giving rise to complex regulatory networks that control diverse pathological and physiological biological activities (12,13). The dysregulation of miRNAs is a common hallmark of oncogenesis and tumor progression (14,15). By evaluating patterns of miRNA expression, it may be possible to better diagnose or monitor specific types of cancer. For example, miR-144 and miR-92a have been shown to serve as valuable and specific diagnostic biomarkers that can guide the detection of specific subtypes of colorectal cancer (16). Additionally, miR-221 can suppress the expression of PHD finger protein 2, thereby influencing liver cancer invasion, leading researchers to highlight this signaling axis as a potential target for therapeutic intervention (17). Efforts have also been made to successfully identify patterns of miRNA dysregulation associated with melanoma (18). Given their essential roles as regulators of cancer onset and progression, further analyses of miRNAs in these oncogenic contexts are warranted.
Tetraspanin cluster of differentiation 82(CD82), also known as Kang-Ai 1 (KAI1), is an important tumor suppressor gene that was first detected based upon analyses of human metastatic prostate cancer samples (19). There is robust evidence linking KAI1 downregulation with the invasive and metastatic activities of various tumors based upon histopathological and molecular analyses (20). KAI1 mutations and associated loss-of-function are evident in a range of tumor types, reaffirming the role of this gene as a tumor suppressor (21–23). KAI1 expression in melanoma has been shown to be associated with tumor grade and patient prognosis, and it has been validated as a risk factor for disease progression (24,25). However, the association between miR-633 and malignant melanoma has not been elucidated to date. The present study investigated the role of miR-633 in the proliferation and migration of melanoma cells. Furthermore, the potential role of miR-633 in regulating KAI1 expression in melanoma cells was explored.
Materials and methods
Prediction of the candidate miRNA associated with KAI1
The online target gene prediction databases TargetScan (http://www.targetscan.org/vert_72), StarBase (http://starbase.sysu.edu.cn) and miRanda (http://www.microrna.orgmicrorna) were used to identify miRNAs that could be associated with KAI1, and to predict the binding region of miRNA-633 to the 3′-UTR of KAI1.
Sample collection
All cancer tissues (n=11) and paracancerous tissues (n=10) used in the present study were collected via surgical resection from patients with melanoma at Cangzhou Central Hospital (Table I). No patients received any treatment, including chemotherapy or radiation therapy, prior to surgery. According to the melanoma treatment guidelines, the resection range was determined based on the different stage, and the paracancerous tissues were taken as close to the outer edge as possible to ensure that it was the adjacent normal tissue. Resected samples were subjected to pathological confirmation, after which they were snap-frozen with liquid nitrogen and stored at −80°C. However, tumor cells were present at the outer edge in one of the patients, so11 cancer tissues and 10 paracancerous tissues were used in the present study. Patients and their families were informed of all study protocols and were asked to sign informed consent forms. The Ethics Committee of Cangzhou Central Hospital approved the present study.
Cell culture and transfection
Normal human primary epidermal melanocytes (HeMn); (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in F-12K medium(Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). A375, A2058, B16, MEL-RM and M21 melanoma cell lines(The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in humidified 5% CO2 incubators at 37°C. A375 and B16 cells were co-transfected with KAI1 wild-type (WT) or KAI1 mutant (MUT), and/or with miR-633 inhibitors or inhibitor-negative control (NC) and miR-633 mimics or mimic-NC. The transfection of cells was performed with the plasmids (Promega Corporation) using Lipofectamine®3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol, with downstream analyses conducted at 24–48 h post-transfection. The sequences of oligo-ribonucleotides were as follows: miR-633 mimics, 5′-CUAAUAGUAUCUACCACAAUAAA-3′; mimic-NC 5′-UUCUCCGAACGUGUCACGUTT-3′; miR-633 inhibitor, 5′-UUUAUUGUGGUAGAUACUAUUAG-3′; and inhibitor-NC, 5′-CAGUACUUUUGUGUAGUACAA-3′.
Reverse transcription-quantitative PCR (RT-qPCR)
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cells and tissues, after which reverse transcription was performed with a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.). After the cDNA concentrations were quantified, qPCR was conducted with SYBR® Premix Ex Taq (Takara Biotechnology Co., Ltd.) following the manufacturer's instructions. GAPDH and U6 were utilized as normalization controls for mRNA and miRNA levels, respectively. Relative fold changes in expression levels were calculated using the 2−ΔΔCq method (26). Primers used were as follows: KAI1, forward 5′-ATCCGATATCCGATCGACATGAGAGGAGTTCGAT-3′ and reverse 5′-CTAGGCGAGATAGACTACCATG-3′; GAPDH, forward 5′-ATCCGATTACCGATACCTAGACC-3′ and reverse 5′-ATGGACTATATCCGACGACGA-3′; miR-633, forward 5′-CCGATACGATGAGAGAAACCCTGA-3′ and reverse 5′-GGACAGAGTTGACTTAAGGCTAGA-3′; and U6, forward 5′-TGCGTTCCCTTTGTCATCCT-3′ and reverse, 5′-AACGCTTCAC-GAATTTGCGT-3′. The thermocycling conditions were as follows: 95°C for 30 sec, followed by 40 cycles of 92°C for 5 sec and 60°C for 30 sec, dissociation at 60°C for 1 min and 95°C for 1 sec.
Dual-luciferase reporter assay
Cells were transfected with KAI1WT or KAI1MUT luciferase reporter plasmids (Promega Corporation), along with miR-633 mimics or control constructs for 24 h at 37°C using Lipofectamine®3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol. Then, a dual-luciferase reporter assay system (Promega Corporation) was utilized to quantify luciferase activity. Renilla luciferase activity was used as the internal control, and data are presented as the ratio of firefly to Renilla luciferase activities.
Cell Counting Kit-8 (CCK-8) assay
Cells were added to 96-well plates (2×103 cells/well). At the indicated time points, 10 µl CCK-8 solution (Dojindo Molecular Technologies, Inc.) was added per well, and absorbance at 450 nm was assessed using a microplate reader (Bio-Rad Laboratories, Inc.).
Wound healing assay
Transfected cells were seeded into 6-well plates at a density of 5×105 cells/well and cultured to confluency in RPMI-1640 medium with 15% FBS at 37°C with 5% CO2. A linear wound was made using a10 µl sterile pipette tip across the confluent cell layer, and the plates were washed twice to remove detached cells and debris. The cells were cultured in RPMI-1640 medium with 2.5% FBS, 24 h after the wound scraping. Then, the size of the wounds was observed and measured at 0 and 24 h under a light microscope (magnification, ×20).
Invasion assay
To assess cell invasion, 3×104 cells were resuspended in serum-free RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and added to the upper portion of a Transwell chamber (Corning Inc.) that had been coated with Matrigel™. At total of 600 µl media containing 20% FBS was added to the lower chamber, and plates were subsequently incubated for 24 h. Cells that had invaded through the matrix to the lower chamber were subsequently fixed for 20 min using 4% paraformaldehyde, after which they were stained for 20 min with crystal violet. The cells were then observed using optical light microscopy (Olympus Corporation). The numbers of invaded cells in five random fields of view were subsequently quantified for each sample.
Western blotting
RIPA buffer (Beyotime Institute of Biotechnology) was used to lyse cells, after which a BCA kit (Beyotime Institute of Biotechnology) was used to quantify protein levels in the lysates. Protein (40 ug/lane) was subsequently subjected to electrophoretic separation (12% SDS-PAGE), transferred to PVDF membranes (EMD Millipore), and blocked with 5% non-fat milk at room temperature for 2 h. The membranes were then incubated overnight with primary antibodies (cat. no. 10205-2-A; 1:1,000; ProteinTech Group, Inc.) at 4°C. Blots were subsequently incubated at room temperature for 2 h with HRP-conjugated secondary antibodies (cat. no. KC-4G3; 1:20,000; Kang Chen Biotech, Inc.), after which ECL (Pierce; Thermo Fisher Scientific, Inc.) was used to detect the protein bands. Image J software (1.48u version; National Institutes of Health) was used for densitometric analyses.
Statistical analysis
All statistical analyses were performed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 (GraphPad Software, Inc.). Data are expressed as the mean ± SD. Multiple group comparisons were analyzed using one-way analysis of variance and Tukey's post hoc test. Independent-samples t-tests were used to analyze the significance of mRNA levels in tumor tissues and adjacent normal tissues. Associations between the expression of pairs of genes were evaluated using Spearman's rank correlation analyses. Independent-samples t-tests were used to analyze the significance of cell proliferation, wound healing/migration and Transwell invasion assay results. All comparisons were two-tailed. P<0.05 was considered to indicate a statistically significant difference.
Results
Identification of miRNAs that potentially target KAI1 in melanoma via bioinformatics prediction analyses
The potential upstream miRNAs that may target KAI1 were selected using the TargetScan, StarBase and miRanda online tools (27). In total, 6 putative regulators of KAI1 (miR-633, miR-362, miR-338, miR-622, miR-203 and miR-197) were identified using this approach (Fig. 1A). The expression levels of these miRNAs were subsequently measured in tumors from patients with melanoma and their matched adjacent normal tissue samples (Fig. 1B). Among the six miRNA candidates, miR-633 was found to be upregulated the most in melanoma tissues compared with adjacent normal tissues.
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression
miR-633 was found to be expressed at higher levels in melanoma cells compared with HeMn control cells (Fig. 2A). Similarly, it was found that miR-633 was upregulated in melanoma tumor tissues compared with adjacent normal tissues, the results were re-plotted in Fig. 2B for ease of reference (Fig. 2B). When the mRNA expression levels for the KAI1 gene were examined in the same samples, the results exhibited an opposite pattern, with KAI1 mRNA expression levels significantly decreased in melanoma tissues compared with normal tissues (Fig. 2C). Pearson correlation analyses confirmed that miR-633 and KAI1 expression was negatively correlated in melanoma tissue samples (Fig. 2D).
miR-633 suppresses KAI1 expression in melanoma
After having identified the predicted miR-633 binding site within the KAI1 3′-UTR, WT and MUT versions of a KAI1 luciferase reporter were constructed (Fig. 3A). Additionally, an miR-633 inhibitor and mimics were prepared, and their transfection efficiency was validated in A375 and B16 cells (Fig. 3B and C). Using luciferase reporter assays, the results confirmed that miR-633 mimics were able to bind to the WT, but not the MUT, version of the KAI1 reporter, consistent with a specific binding interaction between miR-633 and KAI1 (Fig. 3D and E). Subsequently, it was determined that KAI1 expression was decreased at both the mRNA and the protein level in A375 and B16 cells overexpressing miR-633 (Fig. 3F and G). Taken together, these findings confirmed thatmiR-633 may serve as a negative regulator of KAI1 transcription and translation in melanoma cells.
miR-633 enhances melanoma cell proliferation and migration
Finally, the biological importance of miR-633 was assessed in melanoma. Using a CCK-8 assay, it was found that transfection with the miR-633 inhibitor significantly suppressed the numbers of melanoma cells over time compared with cells transfected with NC (Fig. 4A and B), suggesting a role for this miRNA in the proliferation of melanoma cells. In addition, miR-633 inhibitor transfection into both the A375 and B16 melanoma cell lines decreased migration rates in wound healing assays (Fig. 4C) and cell invasion in Transwell assays (Fig. 4D). Collectively, these findings demonstrated that miR-633 was able to promote the migration and proliferation of melanoma cells.
Discussion
In the present study, it was demonstrated that miR-633 was significantly upregulated in melanoma tissue compared with adjacent normal tissue from11patients with melanoma. In addition, a series of melanoma cell lines exhibited a significantly higher level of miR-633 expression compared with HeMn cells. In previous studies, miR-633 was shown to be a functionally important tumor-associated miRNA in lung and brain cancer (28,29). Its functional importance in melanoma, however, had yet to be properly elucidated. The present study used the TargetScan, StarBase and miRanda algorithms to predict 6 candidate miRNAs that specifically targeted KAI1.KAI1 was shown to have one potential complementary miR-633-binding site within its 3′-UTR. Experimental results demonstrated that over expression of miR-633 led to a significant reduction in the levels of KAI1 protein, and deregulated expression of miR-633 significantly altered the proliferation, migration and invasion of A375 and B16 melanoma cells. Taken together, the findings of the present study have provided, to the best of the authors' knowledge, the first evidence thatmiR-633 may function as a tumor promoter in human melanoma, mediated partly through targeting KAI1 expression.
A total of 3,657 mature human miRNAs have been identified to date (miRBase, 2019, http://www.mirbase.org). In prior studies, miRNAs identified from the integumentary system were successfully used as diagnostic and prognostic biomarkers in patients with melanoma (30,31). For example, miR-213 is a suppressor of malignant melanoma progression, thereby indicating that it may be a potentially viable therapeutic target or diagnostic biomarker in patients with this form of cancer (32). Previous studies indicated that deregulation of miR-21 was associated with pro-apoptotic effects in pancreatic and breast cancer cell lines (33). In melanoma, a previous study revealed that miR-205, miR-200 and members of the let-7 family(−125b, −146a, −155, −21, −25, −23a and −29b) were deregulated (34). Previous studies also suggested that abnormal miR-633 expression was associated with the prednisone response early in childhood acute lymphoblastic leukemia relapse (35). However, abnormal miR-633 expression has only been definitively identified in few types of tumors to date. The association between miR-633 and malignant melanoma has not been previously elucidated. The present study demonstrated that the expression levels of miR-633 were upregulated in melanoma tissues and cell lines compared with normal tissues and cells, respectively. Furthermore, the results obtained revealed that deregulation of miR-633 significantly altered the proliferation and migration of A375 and B16 melanoma cells. Therefore, miR-633 may serve as a novel biomarker for melanoma in the future.
KAI1, also known as CD82, is an important transcriptional regulator and tumor suppressor gene, and KAI1 mutations are commonly encountered in a range of tumor types (36). KAI1 regulates chromatin accessibility, in part via the E-cadherin pathway (37), and prior bioinformatics analyses have suggested that KAI1 mutations are associated with lung, bone and brain cancer prognoses, underscoring the relevance of this gene in oncogenic contexts (38). KAI1 may attenuate signaling to shut down metastatic colonization through attenuation of epidermal growth factor receptor signaling and inhibition of the Wnt signaling pathway. You et al (39) suggested that the KAI1 promoter may be regulated by the p53 and NME/NM23 nucleoside diphosphate kinase 1 genes. In pancreatic carcinoma, over expressing KAI1 attenuated the phosphorylation of SRC and STAT3, thereby inhibiting the expression of vascular endothelial growth factor C and limiting the activity of pancreatic carcinoma cells (40). KAI1 has also been shown to serve as a mediator of metabolic reprogramming in tumor cells. For example, KAI1 can suppress the progression of cancers of the digestive system via influencing invasion-associated protein metabolism (41). It also serves as an important tumor suppressor gene in the context of integumental tumors, with KAI1 inactivating mutations being a common feature in human melanoma (42). The ability of KAI1 to suppress oncogenesis is linked to its ability to inhibit the expression of AP-1, JUNB, poly (ADP-ribose) polymerase (PARP) and other oncogenes (43). Khan et al (44) reported that KAI1 is a key regulator of Toll-like receptor 9 (TLR9) trafficking and signaling. KAI1 modulates TLR9-dependent NF-κB nuclear translocation, which is critical for inflammatory cytokine production. A recent study demonstrated that KAI1 was significantly associated with poor survival and could act as an independent prognostic factor in human melanoma for both 5-year and 10-year survival rates (45). KAI1 deregulation has been observed in melanoma cell lines and tumor samples, and the over expression of this protein may significantly reduce melanoma progression (46). Furthermore, miR-203 inhibited frizzled-2 expression via KAI1 expression in human lung carcinoma cells, andKAI1expression may be a useful marker for metastatic, invasive cancer, and prognostic factor in tumors, such as lung cancer (47). However, the specific mechanisms governing KAI1 downregulation in the context of melanoma had yet to be clarified.
The present study aimed to investigate the role of miR-633 in melanoma through its potential interaction with KAI1, revealing that miR-633 may be an important regulator of malignant melanoma. The results showed that miR-633 was upregulated in melanoma cells and tumor tissue samples compared with their controls. In addition, miR-633 inhibition resulted in impaired migration and proliferation of A375 and B16 melanoma cells in vitro. Notably, the results of the dual-luciferase reporter assay demonstrated that miR-633 directly regulated the expression of KAI1. Therefore, the data obtained in the present study have provided important insights into the regulation of KAI1 by miRNAs in cancer cells. miR-633 may serve as a potential candidate for the diagnosis and treatment of human melanoma.
In conclusion, the present study has provided novel evidence to show that inhibition of miR-633 suppresses the proliferation and migration of the malignant melanoma cell lines A375 and B16, partly via regulation of KAI1.
|
|
sec
|
Introduction
Melanoma is one of the most commonly occurring forms of cancer, and malignant melanoma is the third most common skin malignancy (1–6). For most patients with melanoma, immunotherapy, chemotherapy or small-molecule inhibitor administration are not effective therapies (7). Furthermore, the outcomes of patients with advanced-stage disease remain poor (8,9).
MicroRNAs (miRNAs/miRs) are short RNAs (~22 nucleotides in length) that do not encode protein, and yet are important regulators of oncogenesis (10,11). By binding to complementary sequences in the 3′-untranslated region (3′-UTR) of target mRNAs, miRNAs can alter the stability and translation of these transcripts, thereby influencing phenotypic outcomes within cells. A single miRNA can target multiple different mRNAs, giving rise to complex regulatory networks that control diverse pathological and physiological biological activities (12,13). The dysregulation of miRNAs is a common hallmark of oncogenesis and tumor progression (14,15). By evaluating patterns of miRNA expression, it may be possible to better diagnose or monitor specific types of cancer. For example, miR-144 and miR-92a have been shown to serve as valuable and specific diagnostic biomarkers that can guide the detection of specific subtypes of colorectal cancer (16). Additionally, miR-221 can suppress the expression of PHD finger protein 2, thereby influencing liver cancer invasion, leading researchers to highlight this signaling axis as a potential target for therapeutic intervention (17). Efforts have also been made to successfully identify patterns of miRNA dysregulation associated with melanoma (18). Given their essential roles as regulators of cancer onset and progression, further analyses of miRNAs in these oncogenic contexts are warranted.
Tetraspanin cluster of differentiation 82(CD82), also known as Kang-Ai 1 (KAI1), is an important tumor suppressor gene that was first detected based upon analyses of human metastatic prostate cancer samples (19). There is robust evidence linking KAI1 downregulation with the invasive and metastatic activities of various tumors based upon histopathological and molecular analyses (20). KAI1 mutations and associated loss-of-function are evident in a range of tumor types, reaffirming the role of this gene as a tumor suppressor (21–23). KAI1 expression in melanoma has been shown to be associated with tumor grade and patient prognosis, and it has been validated as a risk factor for disease progression (24,25). However, the association between miR-633 and malignant melanoma has not been elucidated to date. The present study investigated the role of miR-633 in the proliferation and migration of melanoma cells. Furthermore, the potential role of miR-633 in regulating KAI1 expression in melanoma cells was explored.
|
|
title
|
Introduction
|
|
p
|
Melanoma is one of the most commonly occurring forms of cancer, and malignant melanoma is the third most common skin malignancy (1–6). For most patients with melanoma, immunotherapy, chemotherapy or small-molecule inhibitor administration are not effective therapies (7). Furthermore, the outcomes of patients with advanced-stage disease remain poor (8,9).
|
|
p
|
MicroRNAs (miRNAs/miRs) are short RNAs (~22 nucleotides in length) that do not encode protein, and yet are important regulators of oncogenesis (10,11). By binding to complementary sequences in the 3′-untranslated region (3′-UTR) of target mRNAs, miRNAs can alter the stability and translation of these transcripts, thereby influencing phenotypic outcomes within cells. A single miRNA can target multiple different mRNAs, giving rise to complex regulatory networks that control diverse pathological and physiological biological activities (12,13). The dysregulation of miRNAs is a common hallmark of oncogenesis and tumor progression (14,15). By evaluating patterns of miRNA expression, it may be possible to better diagnose or monitor specific types of cancer. For example, miR-144 and miR-92a have been shown to serve as valuable and specific diagnostic biomarkers that can guide the detection of specific subtypes of colorectal cancer (16). Additionally, miR-221 can suppress the expression of PHD finger protein 2, thereby influencing liver cancer invasion, leading researchers to highlight this signaling axis as a potential target for therapeutic intervention (17). Efforts have also been made to successfully identify patterns of miRNA dysregulation associated with melanoma (18). Given their essential roles as regulators of cancer onset and progression, further analyses of miRNAs in these oncogenic contexts are warranted.
|
|
p
|
Tetraspanin cluster of differentiation 82(CD82), also known as Kang-Ai 1 (KAI1), is an important tumor suppressor gene that was first detected based upon analyses of human metastatic prostate cancer samples (19). There is robust evidence linking KAI1 downregulation with the invasive and metastatic activities of various tumors based upon histopathological and molecular analyses (20). KAI1 mutations and associated loss-of-function are evident in a range of tumor types, reaffirming the role of this gene as a tumor suppressor (21–23). KAI1 expression in melanoma has been shown to be associated with tumor grade and patient prognosis, and it has been validated as a risk factor for disease progression (24,25). However, the association between miR-633 and malignant melanoma has not been elucidated to date. The present study investigated the role of miR-633 in the proliferation and migration of melanoma cells. Furthermore, the potential role of miR-633 in regulating KAI1 expression in melanoma cells was explored.
|
|
sec
|
Materials and methods
Prediction of the candidate miRNA associated with KAI1
The online target gene prediction databases TargetScan (http://www.targetscan.org/vert_72), StarBase (http://starbase.sysu.edu.cn) and miRanda (http://www.microrna.orgmicrorna) were used to identify miRNAs that could be associated with KAI1, and to predict the binding region of miRNA-633 to the 3′-UTR of KAI1.
Sample collection
All cancer tissues (n=11) and paracancerous tissues (n=10) used in the present study were collected via surgical resection from patients with melanoma at Cangzhou Central Hospital (Table I). No patients received any treatment, including chemotherapy or radiation therapy, prior to surgery. According to the melanoma treatment guidelines, the resection range was determined based on the different stage, and the paracancerous tissues were taken as close to the outer edge as possible to ensure that it was the adjacent normal tissue. Resected samples were subjected to pathological confirmation, after which they were snap-frozen with liquid nitrogen and stored at −80°C. However, tumor cells were present at the outer edge in one of the patients, so11 cancer tissues and 10 paracancerous tissues were used in the present study. Patients and their families were informed of all study protocols and were asked to sign informed consent forms. The Ethics Committee of Cangzhou Central Hospital approved the present study.
Cell culture and transfection
Normal human primary epidermal melanocytes (HeMn); (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in F-12K medium(Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). A375, A2058, B16, MEL-RM and M21 melanoma cell lines(The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in humidified 5% CO2 incubators at 37°C. A375 and B16 cells were co-transfected with KAI1 wild-type (WT) or KAI1 mutant (MUT), and/or with miR-633 inhibitors or inhibitor-negative control (NC) and miR-633 mimics or mimic-NC. The transfection of cells was performed with the plasmids (Promega Corporation) using Lipofectamine®3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol, with downstream analyses conducted at 24–48 h post-transfection. The sequences of oligo-ribonucleotides were as follows: miR-633 mimics, 5′-CUAAUAGUAUCUACCACAAUAAA-3′; mimic-NC 5′-UUCUCCGAACGUGUCACGUTT-3′; miR-633 inhibitor, 5′-UUUAUUGUGGUAGAUACUAUUAG-3′; and inhibitor-NC, 5′-CAGUACUUUUGUGUAGUACAA-3′.
Reverse transcription-quantitative PCR (RT-qPCR)
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cells and tissues, after which reverse transcription was performed with a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.). After the cDNA concentrations were quantified, qPCR was conducted with SYBR® Premix Ex Taq (Takara Biotechnology Co., Ltd.) following the manufacturer's instructions. GAPDH and U6 were utilized as normalization controls for mRNA and miRNA levels, respectively. Relative fold changes in expression levels were calculated using the 2−ΔΔCq method (26). Primers used were as follows: KAI1, forward 5′-ATCCGATATCCGATCGACATGAGAGGAGTTCGAT-3′ and reverse 5′-CTAGGCGAGATAGACTACCATG-3′; GAPDH, forward 5′-ATCCGATTACCGATACCTAGACC-3′ and reverse 5′-ATGGACTATATCCGACGACGA-3′; miR-633, forward 5′-CCGATACGATGAGAGAAACCCTGA-3′ and reverse 5′-GGACAGAGTTGACTTAAGGCTAGA-3′; and U6, forward 5′-TGCGTTCCCTTTGTCATCCT-3′ and reverse, 5′-AACGCTTCAC-GAATTTGCGT-3′. The thermocycling conditions were as follows: 95°C for 30 sec, followed by 40 cycles of 92°C for 5 sec and 60°C for 30 sec, dissociation at 60°C for 1 min and 95°C for 1 sec.
Dual-luciferase reporter assay
Cells were transfected with KAI1WT or KAI1MUT luciferase reporter plasmids (Promega Corporation), along with miR-633 mimics or control constructs for 24 h at 37°C using Lipofectamine®3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol. Then, a dual-luciferase reporter assay system (Promega Corporation) was utilized to quantify luciferase activity. Renilla luciferase activity was used as the internal control, and data are presented as the ratio of firefly to Renilla luciferase activities.
Cell Counting Kit-8 (CCK-8) assay
Cells were added to 96-well plates (2×103 cells/well). At the indicated time points, 10 µl CCK-8 solution (Dojindo Molecular Technologies, Inc.) was added per well, and absorbance at 450 nm was assessed using a microplate reader (Bio-Rad Laboratories, Inc.).
Wound healing assay
Transfected cells were seeded into 6-well plates at a density of 5×105 cells/well and cultured to confluency in RPMI-1640 medium with 15% FBS at 37°C with 5% CO2. A linear wound was made using a10 µl sterile pipette tip across the confluent cell layer, and the plates were washed twice to remove detached cells and debris. The cells were cultured in RPMI-1640 medium with 2.5% FBS, 24 h after the wound scraping. Then, the size of the wounds was observed and measured at 0 and 24 h under a light microscope (magnification, ×20).
Invasion assay
To assess cell invasion, 3×104 cells were resuspended in serum-free RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and added to the upper portion of a Transwell chamber (Corning Inc.) that had been coated with Matrigel™. At total of 600 µl media containing 20% FBS was added to the lower chamber, and plates were subsequently incubated for 24 h. Cells that had invaded through the matrix to the lower chamber were subsequently fixed for 20 min using 4% paraformaldehyde, after which they were stained for 20 min with crystal violet. The cells were then observed using optical light microscopy (Olympus Corporation). The numbers of invaded cells in five random fields of view were subsequently quantified for each sample.
Western blotting
RIPA buffer (Beyotime Institute of Biotechnology) was used to lyse cells, after which a BCA kit (Beyotime Institute of Biotechnology) was used to quantify protein levels in the lysates. Protein (40 ug/lane) was subsequently subjected to electrophoretic separation (12% SDS-PAGE), transferred to PVDF membranes (EMD Millipore), and blocked with 5% non-fat milk at room temperature for 2 h. The membranes were then incubated overnight with primary antibodies (cat. no. 10205-2-A; 1:1,000; ProteinTech Group, Inc.) at 4°C. Blots were subsequently incubated at room temperature for 2 h with HRP-conjugated secondary antibodies (cat. no. KC-4G3; 1:20,000; Kang Chen Biotech, Inc.), after which ECL (Pierce; Thermo Fisher Scientific, Inc.) was used to detect the protein bands. Image J software (1.48u version; National Institutes of Health) was used for densitometric analyses.
Statistical analysis
All statistical analyses were performed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 (GraphPad Software, Inc.). Data are expressed as the mean ± SD. Multiple group comparisons were analyzed using one-way analysis of variance and Tukey's post hoc test. Independent-samples t-tests were used to analyze the significance of mRNA levels in tumor tissues and adjacent normal tissues. Associations between the expression of pairs of genes were evaluated using Spearman's rank correlation analyses. Independent-samples t-tests were used to analyze the significance of cell proliferation, wound healing/migration and Transwell invasion assay results. All comparisons were two-tailed. P<0.05 was considered to indicate a statistically significant difference.
|
|
title
|
Materials and methods
|
|
sec
|
Prediction of the candidate miRNA associated with KAI1
The online target gene prediction databases TargetScan (http://www.targetscan.org/vert_72), StarBase (http://starbase.sysu.edu.cn) and miRanda (http://www.microrna.orgmicrorna) were used to identify miRNAs that could be associated with KAI1, and to predict the binding region of miRNA-633 to the 3′-UTR of KAI1.
Sample collection
All cancer tissues (n=11) and paracancerous tissues (n=10) used in the present study were collected via surgical resection from patients with melanoma at Cangzhou Central Hospital (Table I). No patients received any treatment, including chemotherapy or radiation therapy, prior to surgery. According to the melanoma treatment guidelines, the resection range was determined based on the different stage, and the paracancerous tissues were taken as close to the outer edge as possible to ensure that it was the adjacent normal tissue. Resected samples were subjected to pathological confirmation, after which they were snap-frozen with liquid nitrogen and stored at −80°C. However, tumor cells were present at the outer edge in one of the patients, so11 cancer tissues and 10 paracancerous tissues were used in the present study. Patients and their families were informed of all study protocols and were asked to sign informed consent forms. The Ethics Committee of Cangzhou Central Hospital approved the present study.
Cell culture and transfection
Normal human primary epidermal melanocytes (HeMn); (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in F-12K medium(Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). A375, A2058, B16, MEL-RM and M21 melanoma cell lines(The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in humidified 5% CO2 incubators at 37°C. A375 and B16 cells were co-transfected with KAI1 wild-type (WT) or KAI1 mutant (MUT), and/or with miR-633 inhibitors or inhibitor-negative control (NC) and miR-633 mimics or mimic-NC. The transfection of cells was performed with the plasmids (Promega Corporation) using Lipofectamine®3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol, with downstream analyses conducted at 24–48 h post-transfection. The sequences of oligo-ribonucleotides were as follows: miR-633 mimics, 5′-CUAAUAGUAUCUACCACAAUAAA-3′; mimic-NC 5′-UUCUCCGAACGUGUCACGUTT-3′; miR-633 inhibitor, 5′-UUUAUUGUGGUAGAUACUAUUAG-3′; and inhibitor-NC, 5′-CAGUACUUUUGUGUAGUACAA-3′.
Reverse transcription-quantitative PCR (RT-qPCR)
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cells and tissues, after which reverse transcription was performed with a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.). After the cDNA concentrations were quantified, qPCR was conducted with SYBR® Premix Ex Taq (Takara Biotechnology Co., Ltd.) following the manufacturer's instructions. GAPDH and U6 were utilized as normalization controls for mRNA and miRNA levels, respectively. Relative fold changes in expression levels were calculated using the 2−ΔΔCq method (26). Primers used were as follows: KAI1, forward 5′-ATCCGATATCCGATCGACATGAGAGGAGTTCGAT-3′ and reverse 5′-CTAGGCGAGATAGACTACCATG-3′; GAPDH, forward 5′-ATCCGATTACCGATACCTAGACC-3′ and reverse 5′-ATGGACTATATCCGACGACGA-3′; miR-633, forward 5′-CCGATACGATGAGAGAAACCCTGA-3′ and reverse 5′-GGACAGAGTTGACTTAAGGCTAGA-3′; and U6, forward 5′-TGCGTTCCCTTTGTCATCCT-3′ and reverse, 5′-AACGCTTCAC-GAATTTGCGT-3′. The thermocycling conditions were as follows: 95°C for 30 sec, followed by 40 cycles of 92°C for 5 sec and 60°C for 30 sec, dissociation at 60°C for 1 min and 95°C for 1 sec.
Dual-luciferase reporter assay
Cells were transfected with KAI1WT or KAI1MUT luciferase reporter plasmids (Promega Corporation), along with miR-633 mimics or control constructs for 24 h at 37°C using Lipofectamine®3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol. Then, a dual-luciferase reporter assay system (Promega Corporation) was utilized to quantify luciferase activity. Renilla luciferase activity was used as the internal control, and data are presented as the ratio of firefly to Renilla luciferase activities.
Cell Counting Kit-8 (CCK-8) assay
Cells were added to 96-well plates (2×103 cells/well). At the indicated time points, 10 µl CCK-8 solution (Dojindo Molecular Technologies, Inc.) was added per well, and absorbance at 450 nm was assessed using a microplate reader (Bio-Rad Laboratories, Inc.).
Wound healing assay
Transfected cells were seeded into 6-well plates at a density of 5×105 cells/well and cultured to confluency in RPMI-1640 medium with 15% FBS at 37°C with 5% CO2. A linear wound was made using a10 µl sterile pipette tip across the confluent cell layer, and the plates were washed twice to remove detached cells and debris. The cells were cultured in RPMI-1640 medium with 2.5% FBS, 24 h after the wound scraping. Then, the size of the wounds was observed and measured at 0 and 24 h under a light microscope (magnification, ×20).
Invasion assay
To assess cell invasion, 3×104 cells were resuspended in serum-free RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and added to the upper portion of a Transwell chamber (Corning Inc.) that had been coated with Matrigel™. At total of 600 µl media containing 20% FBS was added to the lower chamber, and plates were subsequently incubated for 24 h. Cells that had invaded through the matrix to the lower chamber were subsequently fixed for 20 min using 4% paraformaldehyde, after which they were stained for 20 min with crystal violet. The cells were then observed using optical light microscopy (Olympus Corporation). The numbers of invaded cells in five random fields of view were subsequently quantified for each sample.
Western blotting
RIPA buffer (Beyotime Institute of Biotechnology) was used to lyse cells, after which a BCA kit (Beyotime Institute of Biotechnology) was used to quantify protein levels in the lysates. Protein (40 ug/lane) was subsequently subjected to electrophoretic separation (12% SDS-PAGE), transferred to PVDF membranes (EMD Millipore), and blocked with 5% non-fat milk at room temperature for 2 h. The membranes were then incubated overnight with primary antibodies (cat. no. 10205-2-A; 1:1,000; ProteinTech Group, Inc.) at 4°C. Blots were subsequently incubated at room temperature for 2 h with HRP-conjugated secondary antibodies (cat. no. KC-4G3; 1:20,000; Kang Chen Biotech, Inc.), after which ECL (Pierce; Thermo Fisher Scientific, Inc.) was used to detect the protein bands. Image J software (1.48u version; National Institutes of Health) was used for densitometric analyses.
Statistical analysis
All statistical analyses were performed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 (GraphPad Software, Inc.). Data are expressed as the mean ± SD. Multiple group comparisons were analyzed using one-way analysis of variance and Tukey's post hoc test. Independent-samples t-tests were used to analyze the significance of mRNA levels in tumor tissues and adjacent normal tissues. Associations between the expression of pairs of genes were evaluated using Spearman's rank correlation analyses. Independent-samples t-tests were used to analyze the significance of cell proliferation, wound healing/migration and Transwell invasion assay results. All comparisons were two-tailed. P<0.05 was considered to indicate a statistically significant difference.
|
|
sec
|
Prediction of the candidate miRNA associated with KAI1
The online target gene prediction databases TargetScan (http://www.targetscan.org/vert_72), StarBase (http://starbase.sysu.edu.cn) and miRanda (http://www.microrna.orgmicrorna) were used to identify miRNAs that could be associated with KAI1, and to predict the binding region of miRNA-633 to the 3′-UTR of KAI1.
|
|
title
|
Prediction of the candidate miRNA associated with KAI1
|
|
p
|
The online target gene prediction databases TargetScan (http://www.targetscan.org/vert_72), StarBase (http://starbase.sysu.edu.cn) and miRanda (http://www.microrna.orgmicrorna) were used to identify miRNAs that could be associated with KAI1, and to predict the binding region of miRNA-633 to the 3′-UTR of KAI1.
|
|
sec
|
Sample collection
All cancer tissues (n=11) and paracancerous tissues (n=10) used in the present study were collected via surgical resection from patients with melanoma at Cangzhou Central Hospital (Table I). No patients received any treatment, including chemotherapy or radiation therapy, prior to surgery. According to the melanoma treatment guidelines, the resection range was determined based on the different stage, and the paracancerous tissues were taken as close to the outer edge as possible to ensure that it was the adjacent normal tissue. Resected samples were subjected to pathological confirmation, after which they were snap-frozen with liquid nitrogen and stored at −80°C. However, tumor cells were present at the outer edge in one of the patients, so11 cancer tissues and 10 paracancerous tissues were used in the present study. Patients and their families were informed of all study protocols and were asked to sign informed consent forms. The Ethics Committee of Cangzhou Central Hospital approved the present study.
|
|
title
|
Sample collection
|
|
p
|
All cancer tissues (n=11) and paracancerous tissues (n=10) used in the present study were collected via surgical resection from patients with melanoma at Cangzhou Central Hospital (Table I). No patients received any treatment, including chemotherapy or radiation therapy, prior to surgery. According to the melanoma treatment guidelines, the resection range was determined based on the different stage, and the paracancerous tissues were taken as close to the outer edge as possible to ensure that it was the adjacent normal tissue. Resected samples were subjected to pathological confirmation, after which they were snap-frozen with liquid nitrogen and stored at −80°C. However, tumor cells were present at the outer edge in one of the patients, so11 cancer tissues and 10 paracancerous tissues were used in the present study. Patients and their families were informed of all study protocols and were asked to sign informed consent forms. The Ethics Committee of Cangzhou Central Hospital approved the present study.
|
|
sec
|
Cell culture and transfection
Normal human primary epidermal melanocytes (HeMn); (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in F-12K medium(Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). A375, A2058, B16, MEL-RM and M21 melanoma cell lines(The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in humidified 5% CO2 incubators at 37°C. A375 and B16 cells were co-transfected with KAI1 wild-type (WT) or KAI1 mutant (MUT), and/or with miR-633 inhibitors or inhibitor-negative control (NC) and miR-633 mimics or mimic-NC. The transfection of cells was performed with the plasmids (Promega Corporation) using Lipofectamine®3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol, with downstream analyses conducted at 24–48 h post-transfection. The sequences of oligo-ribonucleotides were as follows: miR-633 mimics, 5′-CUAAUAGUAUCUACCACAAUAAA-3′; mimic-NC 5′-UUCUCCGAACGUGUCACGUTT-3′; miR-633 inhibitor, 5′-UUUAUUGUGGUAGAUACUAUUAG-3′; and inhibitor-NC, 5′-CAGUACUUUUGUGUAGUACAA-3′.
|
|
title
|
Cell culture and transfection
|
|
p
|
Normal human primary epidermal melanocytes (HeMn); (The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in F-12K medium(Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). A375, A2058, B16, MEL-RM and M21 melanoma cell lines(The Cell Bank of Type Culture Collection of Chinese Academy of Sciences) were maintained in RPMI-1640 media supplemented with 10% fetal bovine serum (both purchased from Gibco; Thermo Fisher Scientific, Inc.). All cells were grown in humidified 5% CO2 incubators at 37°C. A375 and B16 cells were co-transfected with KAI1 wild-type (WT) or KAI1 mutant (MUT), and/or with miR-633 inhibitors or inhibitor-negative control (NC) and miR-633 mimics or mimic-NC. The transfection of cells was performed with the plasmids (Promega Corporation) using Lipofectamine®3000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol, with downstream analyses conducted at 24–48 h post-transfection. The sequences of oligo-ribonucleotides were as follows: miR-633 mimics, 5′-CUAAUAGUAUCUACCACAAUAAA-3′; mimic-NC 5′-UUCUCCGAACGUGUCACGUTT-3′; miR-633 inhibitor, 5′-UUUAUUGUGGUAGAUACUAUUAG-3′; and inhibitor-NC, 5′-CAGUACUUUUGUGUAGUACAA-3′.
|
|
sec
|
Reverse transcription-quantitative PCR (RT-qPCR)
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cells and tissues, after which reverse transcription was performed with a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.). After the cDNA concentrations were quantified, qPCR was conducted with SYBR® Premix Ex Taq (Takara Biotechnology Co., Ltd.) following the manufacturer's instructions. GAPDH and U6 were utilized as normalization controls for mRNA and miRNA levels, respectively. Relative fold changes in expression levels were calculated using the 2−ΔΔCq method (26). Primers used were as follows: KAI1, forward 5′-ATCCGATATCCGATCGACATGAGAGGAGTTCGAT-3′ and reverse 5′-CTAGGCGAGATAGACTACCATG-3′; GAPDH, forward 5′-ATCCGATTACCGATACCTAGACC-3′ and reverse 5′-ATGGACTATATCCGACGACGA-3′; miR-633, forward 5′-CCGATACGATGAGAGAAACCCTGA-3′ and reverse 5′-GGACAGAGTTGACTTAAGGCTAGA-3′; and U6, forward 5′-TGCGTTCCCTTTGTCATCCT-3′ and reverse, 5′-AACGCTTCAC-GAATTTGCGT-3′. The thermocycling conditions were as follows: 95°C for 30 sec, followed by 40 cycles of 92°C for 5 sec and 60°C for 30 sec, dissociation at 60°C for 1 min and 95°C for 1 sec.
|
|
title
|
Reverse transcription-quantitative PCR (RT-qPCR)
|
|
p
|
TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from the cells and tissues, after which reverse transcription was performed with a PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd.). After the cDNA concentrations were quantified, qPCR was conducted with SYBR® Premix Ex Taq (Takara Biotechnology Co., Ltd.) following the manufacturer's instructions. GAPDH and U6 were utilized as normalization controls for mRNA and miRNA levels, respectively. Relative fold changes in expression levels were calculated using the 2−ΔΔCq method (26). Primers used were as follows: KAI1, forward 5′-ATCCGATATCCGATCGACATGAGAGGAGTTCGAT-3′ and reverse 5′-CTAGGCGAGATAGACTACCATG-3′; GAPDH, forward 5′-ATCCGATTACCGATACCTAGACC-3′ and reverse 5′-ATGGACTATATCCGACGACGA-3′; miR-633, forward 5′-CCGATACGATGAGAGAAACCCTGA-3′ and reverse 5′-GGACAGAGTTGACTTAAGGCTAGA-3′; and U6, forward 5′-TGCGTTCCCTTTGTCATCCT-3′ and reverse, 5′-AACGCTTCAC-GAATTTGCGT-3′. The thermocycling conditions were as follows: 95°C for 30 sec, followed by 40 cycles of 92°C for 5 sec and 60°C for 30 sec, dissociation at 60°C for 1 min and 95°C for 1 sec.
|
|
sec
|
Dual-luciferase reporter assay
Cells were transfected with KAI1WT or KAI1MUT luciferase reporter plasmids (Promega Corporation), along with miR-633 mimics or control constructs for 24 h at 37°C using Lipofectamine®3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol. Then, a dual-luciferase reporter assay system (Promega Corporation) was utilized to quantify luciferase activity. Renilla luciferase activity was used as the internal control, and data are presented as the ratio of firefly to Renilla luciferase activities.
|
|
title
|
Dual-luciferase reporter assay
|
|
p
|
Cells were transfected with KAI1WT or KAI1MUT luciferase reporter plasmids (Promega Corporation), along with miR-633 mimics or control constructs for 24 h at 37°C using Lipofectamine®3000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's protocol. Then, a dual-luciferase reporter assay system (Promega Corporation) was utilized to quantify luciferase activity. Renilla luciferase activity was used as the internal control, and data are presented as the ratio of firefly to Renilla luciferase activities.
|
|
sec
|
Cell Counting Kit-8 (CCK-8) assay
Cells were added to 96-well plates (2×103 cells/well). At the indicated time points, 10 µl CCK-8 solution (Dojindo Molecular Technologies, Inc.) was added per well, and absorbance at 450 nm was assessed using a microplate reader (Bio-Rad Laboratories, Inc.).
|
|
title
|
Cell Counting Kit-8 (CCK-8) assay
|
|
p
|
Cells were added to 96-well plates (2×103 cells/well). At the indicated time points, 10 µl CCK-8 solution (Dojindo Molecular Technologies, Inc.) was added per well, and absorbance at 450 nm was assessed using a microplate reader (Bio-Rad Laboratories, Inc.).
|
|
sec
|
Wound healing assay
Transfected cells were seeded into 6-well plates at a density of 5×105 cells/well and cultured to confluency in RPMI-1640 medium with 15% FBS at 37°C with 5% CO2. A linear wound was made using a10 µl sterile pipette tip across the confluent cell layer, and the plates were washed twice to remove detached cells and debris. The cells were cultured in RPMI-1640 medium with 2.5% FBS, 24 h after the wound scraping. Then, the size of the wounds was observed and measured at 0 and 24 h under a light microscope (magnification, ×20).
|
|
title
|
Wound healing assay
|
|
p
|
Transfected cells were seeded into 6-well plates at a density of 5×105 cells/well and cultured to confluency in RPMI-1640 medium with 15% FBS at 37°C with 5% CO2. A linear wound was made using a10 µl sterile pipette tip across the confluent cell layer, and the plates were washed twice to remove detached cells and debris. The cells were cultured in RPMI-1640 medium with 2.5% FBS, 24 h after the wound scraping. Then, the size of the wounds was observed and measured at 0 and 24 h under a light microscope (magnification, ×20).
|
|
sec
|
Invasion assay
To assess cell invasion, 3×104 cells were resuspended in serum-free RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and added to the upper portion of a Transwell chamber (Corning Inc.) that had been coated with Matrigel™. At total of 600 µl media containing 20% FBS was added to the lower chamber, and plates were subsequently incubated for 24 h. Cells that had invaded through the matrix to the lower chamber were subsequently fixed for 20 min using 4% paraformaldehyde, after which they were stained for 20 min with crystal violet. The cells were then observed using optical light microscopy (Olympus Corporation). The numbers of invaded cells in five random fields of view were subsequently quantified for each sample.
|
|
title
|
Invasion assay
|
|
p
|
To assess cell invasion, 3×104 cells were resuspended in serum-free RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) and added to the upper portion of a Transwell chamber (Corning Inc.) that had been coated with Matrigel™. At total of 600 µl media containing 20% FBS was added to the lower chamber, and plates were subsequently incubated for 24 h. Cells that had invaded through the matrix to the lower chamber were subsequently fixed for 20 min using 4% paraformaldehyde, after which they were stained for 20 min with crystal violet. The cells were then observed using optical light microscopy (Olympus Corporation). The numbers of invaded cells in five random fields of view were subsequently quantified for each sample.
|
|
sec
|
Western blotting
RIPA buffer (Beyotime Institute of Biotechnology) was used to lyse cells, after which a BCA kit (Beyotime Institute of Biotechnology) was used to quantify protein levels in the lysates. Protein (40 ug/lane) was subsequently subjected to electrophoretic separation (12% SDS-PAGE), transferred to PVDF membranes (EMD Millipore), and blocked with 5% non-fat milk at room temperature for 2 h. The membranes were then incubated overnight with primary antibodies (cat. no. 10205-2-A; 1:1,000; ProteinTech Group, Inc.) at 4°C. Blots were subsequently incubated at room temperature for 2 h with HRP-conjugated secondary antibodies (cat. no. KC-4G3; 1:20,000; Kang Chen Biotech, Inc.), after which ECL (Pierce; Thermo Fisher Scientific, Inc.) was used to detect the protein bands. Image J software (1.48u version; National Institutes of Health) was used for densitometric analyses.
|
|
title
|
Western blotting
|
|
p
|
RIPA buffer (Beyotime Institute of Biotechnology) was used to lyse cells, after which a BCA kit (Beyotime Institute of Biotechnology) was used to quantify protein levels in the lysates. Protein (40 ug/lane) was subsequently subjected to electrophoretic separation (12% SDS-PAGE), transferred to PVDF membranes (EMD Millipore), and blocked with 5% non-fat milk at room temperature for 2 h. The membranes were then incubated overnight with primary antibodies (cat. no. 10205-2-A; 1:1,000; ProteinTech Group, Inc.) at 4°C. Blots were subsequently incubated at room temperature for 2 h with HRP-conjugated secondary antibodies (cat. no. KC-4G3; 1:20,000; Kang Chen Biotech, Inc.), after which ECL (Pierce; Thermo Fisher Scientific, Inc.) was used to detect the protein bands. Image J software (1.48u version; National Institutes of Health) was used for densitometric analyses.
|
|
sec
|
Statistical analysis
All statistical analyses were performed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 (GraphPad Software, Inc.). Data are expressed as the mean ± SD. Multiple group comparisons were analyzed using one-way analysis of variance and Tukey's post hoc test. Independent-samples t-tests were used to analyze the significance of mRNA levels in tumor tissues and adjacent normal tissues. Associations between the expression of pairs of genes were evaluated using Spearman's rank correlation analyses. Independent-samples t-tests were used to analyze the significance of cell proliferation, wound healing/migration and Transwell invasion assay results. All comparisons were two-tailed. P<0.05 was considered to indicate a statistically significant difference.
|
|
title
|
Statistical analysis
|
|
p
|
All statistical analyses were performed using SPSS 21.0 (IBM Corp.) and GraphPad Prism 7.0 (GraphPad Software, Inc.). Data are expressed as the mean ± SD. Multiple group comparisons were analyzed using one-way analysis of variance and Tukey's post hoc test. Independent-samples t-tests were used to analyze the significance of mRNA levels in tumor tissues and adjacent normal tissues. Associations between the expression of pairs of genes were evaluated using Spearman's rank correlation analyses. Independent-samples t-tests were used to analyze the significance of cell proliferation, wound healing/migration and Transwell invasion assay results. All comparisons were two-tailed. P<0.05 was considered to indicate a statistically significant difference.
|
|
sec
|
Results
Identification of miRNAs that potentially target KAI1 in melanoma via bioinformatics prediction analyses
The potential upstream miRNAs that may target KAI1 were selected using the TargetScan, StarBase and miRanda online tools (27). In total, 6 putative regulators of KAI1 (miR-633, miR-362, miR-338, miR-622, miR-203 and miR-197) were identified using this approach (Fig. 1A). The expression levels of these miRNAs were subsequently measured in tumors from patients with melanoma and their matched adjacent normal tissue samples (Fig. 1B). Among the six miRNA candidates, miR-633 was found to be upregulated the most in melanoma tissues compared with adjacent normal tissues.
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression
miR-633 was found to be expressed at higher levels in melanoma cells compared with HeMn control cells (Fig. 2A). Similarly, it was found that miR-633 was upregulated in melanoma tumor tissues compared with adjacent normal tissues, the results were re-plotted in Fig. 2B for ease of reference (Fig. 2B). When the mRNA expression levels for the KAI1 gene were examined in the same samples, the results exhibited an opposite pattern, with KAI1 mRNA expression levels significantly decreased in melanoma tissues compared with normal tissues (Fig. 2C). Pearson correlation analyses confirmed that miR-633 and KAI1 expression was negatively correlated in melanoma tissue samples (Fig. 2D).
miR-633 suppresses KAI1 expression in melanoma
After having identified the predicted miR-633 binding site within the KAI1 3′-UTR, WT and MUT versions of a KAI1 luciferase reporter were constructed (Fig. 3A). Additionally, an miR-633 inhibitor and mimics were prepared, and their transfection efficiency was validated in A375 and B16 cells (Fig. 3B and C). Using luciferase reporter assays, the results confirmed that miR-633 mimics were able to bind to the WT, but not the MUT, version of the KAI1 reporter, consistent with a specific binding interaction between miR-633 and KAI1 (Fig. 3D and E). Subsequently, it was determined that KAI1 expression was decreased at both the mRNA and the protein level in A375 and B16 cells overexpressing miR-633 (Fig. 3F and G). Taken together, these findings confirmed thatmiR-633 may serve as a negative regulator of KAI1 transcription and translation in melanoma cells.
miR-633 enhances melanoma cell proliferation and migration
Finally, the biological importance of miR-633 was assessed in melanoma. Using a CCK-8 assay, it was found that transfection with the miR-633 inhibitor significantly suppressed the numbers of melanoma cells over time compared with cells transfected with NC (Fig. 4A and B), suggesting a role for this miRNA in the proliferation of melanoma cells. In addition, miR-633 inhibitor transfection into both the A375 and B16 melanoma cell lines decreased migration rates in wound healing assays (Fig. 4C) and cell invasion in Transwell assays (Fig. 4D). Collectively, these findings demonstrated that miR-633 was able to promote the migration and proliferation of melanoma cells.
|
|
title
|
Results
|
|
sec
|
Identification of miRNAs that potentially target KAI1 in melanoma via bioinformatics prediction analyses
The potential upstream miRNAs that may target KAI1 were selected using the TargetScan, StarBase and miRanda online tools (27). In total, 6 putative regulators of KAI1 (miR-633, miR-362, miR-338, miR-622, miR-203 and miR-197) were identified using this approach (Fig. 1A). The expression levels of these miRNAs were subsequently measured in tumors from patients with melanoma and their matched adjacent normal tissue samples (Fig. 1B). Among the six miRNA candidates, miR-633 was found to be upregulated the most in melanoma tissues compared with adjacent normal tissues.
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression
miR-633 was found to be expressed at higher levels in melanoma cells compared with HeMn control cells (Fig. 2A). Similarly, it was found that miR-633 was upregulated in melanoma tumor tissues compared with adjacent normal tissues, the results were re-plotted in Fig. 2B for ease of reference (Fig. 2B). When the mRNA expression levels for the KAI1 gene were examined in the same samples, the results exhibited an opposite pattern, with KAI1 mRNA expression levels significantly decreased in melanoma tissues compared with normal tissues (Fig. 2C). Pearson correlation analyses confirmed that miR-633 and KAI1 expression was negatively correlated in melanoma tissue samples (Fig. 2D).
miR-633 suppresses KAI1 expression in melanoma
After having identified the predicted miR-633 binding site within the KAI1 3′-UTR, WT and MUT versions of a KAI1 luciferase reporter were constructed (Fig. 3A). Additionally, an miR-633 inhibitor and mimics were prepared, and their transfection efficiency was validated in A375 and B16 cells (Fig. 3B and C). Using luciferase reporter assays, the results confirmed that miR-633 mimics were able to bind to the WT, but not the MUT, version of the KAI1 reporter, consistent with a specific binding interaction between miR-633 and KAI1 (Fig. 3D and E). Subsequently, it was determined that KAI1 expression was decreased at both the mRNA and the protein level in A375 and B16 cells overexpressing miR-633 (Fig. 3F and G). Taken together, these findings confirmed thatmiR-633 may serve as a negative regulator of KAI1 transcription and translation in melanoma cells.
miR-633 enhances melanoma cell proliferation and migration
Finally, the biological importance of miR-633 was assessed in melanoma. Using a CCK-8 assay, it was found that transfection with the miR-633 inhibitor significantly suppressed the numbers of melanoma cells over time compared with cells transfected with NC (Fig. 4A and B), suggesting a role for this miRNA in the proliferation of melanoma cells. In addition, miR-633 inhibitor transfection into both the A375 and B16 melanoma cell lines decreased migration rates in wound healing assays (Fig. 4C) and cell invasion in Transwell assays (Fig. 4D). Collectively, these findings demonstrated that miR-633 was able to promote the migration and proliferation of melanoma cells.
|
|
sec
|
Identification of miRNAs that potentially target KAI1 in melanoma via bioinformatics prediction analyses
The potential upstream miRNAs that may target KAI1 were selected using the TargetScan, StarBase and miRanda online tools (27). In total, 6 putative regulators of KAI1 (miR-633, miR-362, miR-338, miR-622, miR-203 and miR-197) were identified using this approach (Fig. 1A). The expression levels of these miRNAs were subsequently measured in tumors from patients with melanoma and their matched adjacent normal tissue samples (Fig. 1B). Among the six miRNA candidates, miR-633 was found to be upregulated the most in melanoma tissues compared with adjacent normal tissues.
|
|
title
|
Identification of miRNAs that potentially target KAI1 in melanoma via bioinformatics prediction analyses
|
|
p
|
The potential upstream miRNAs that may target KAI1 were selected using the TargetScan, StarBase and miRanda online tools (27). In total, 6 putative regulators of KAI1 (miR-633, miR-362, miR-338, miR-622, miR-203 and miR-197) were identified using this approach (Fig. 1A). The expression levels of these miRNAs were subsequently measured in tumors from patients with melanoma and their matched adjacent normal tissue samples (Fig. 1B). Among the six miRNA candidates, miR-633 was found to be upregulated the most in melanoma tissues compared with adjacent normal tissues.
|
|
sec
|
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression
miR-633 was found to be expressed at higher levels in melanoma cells compared with HeMn control cells (Fig. 2A). Similarly, it was found that miR-633 was upregulated in melanoma tumor tissues compared with adjacent normal tissues, the results were re-plotted in Fig. 2B for ease of reference (Fig. 2B). When the mRNA expression levels for the KAI1 gene were examined in the same samples, the results exhibited an opposite pattern, with KAI1 mRNA expression levels significantly decreased in melanoma tissues compared with normal tissues (Fig. 2C). Pearson correlation analyses confirmed that miR-633 and KAI1 expression was negatively correlated in melanoma tissue samples (Fig. 2D).
|
|
title
|
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression
|
|
p
|
miR-633 was found to be expressed at higher levels in melanoma cells compared with HeMn control cells (Fig. 2A). Similarly, it was found that miR-633 was upregulated in melanoma tumor tissues compared with adjacent normal tissues, the results were re-plotted in Fig. 2B for ease of reference (Fig. 2B). When the mRNA expression levels for the KAI1 gene were examined in the same samples, the results exhibited an opposite pattern, with KAI1 mRNA expression levels significantly decreased in melanoma tissues compared with normal tissues (Fig. 2C). Pearson correlation analyses confirmed that miR-633 and KAI1 expression was negatively correlated in melanoma tissue samples (Fig. 2D).
|
|
sec
|
miR-633 suppresses KAI1 expression in melanoma
After having identified the predicted miR-633 binding site within the KAI1 3′-UTR, WT and MUT versions of a KAI1 luciferase reporter were constructed (Fig. 3A). Additionally, an miR-633 inhibitor and mimics were prepared, and their transfection efficiency was validated in A375 and B16 cells (Fig. 3B and C). Using luciferase reporter assays, the results confirmed that miR-633 mimics were able to bind to the WT, but not the MUT, version of the KAI1 reporter, consistent with a specific binding interaction between miR-633 and KAI1 (Fig. 3D and E). Subsequently, it was determined that KAI1 expression was decreased at both the mRNA and the protein level in A375 and B16 cells overexpressing miR-633 (Fig. 3F and G). Taken together, these findings confirmed thatmiR-633 may serve as a negative regulator of KAI1 transcription and translation in melanoma cells.
|
|
title
|
miR-633 suppresses KAI1 expression in melanoma
|
|
p
|
After having identified the predicted miR-633 binding site within the KAI1 3′-UTR, WT and MUT versions of a KAI1 luciferase reporter were constructed (Fig. 3A). Additionally, an miR-633 inhibitor and mimics were prepared, and their transfection efficiency was validated in A375 and B16 cells (Fig. 3B and C). Using luciferase reporter assays, the results confirmed that miR-633 mimics were able to bind to the WT, but not the MUT, version of the KAI1 reporter, consistent with a specific binding interaction between miR-633 and KAI1 (Fig. 3D and E). Subsequently, it was determined that KAI1 expression was decreased at both the mRNA and the protein level in A375 and B16 cells overexpressing miR-633 (Fig. 3F and G). Taken together, these findings confirmed thatmiR-633 may serve as a negative regulator of KAI1 transcription and translation in melanoma cells.
|
|
sec
|
miR-633 enhances melanoma cell proliferation and migration
Finally, the biological importance of miR-633 was assessed in melanoma. Using a CCK-8 assay, it was found that transfection with the miR-633 inhibitor significantly suppressed the numbers of melanoma cells over time compared with cells transfected with NC (Fig. 4A and B), suggesting a role for this miRNA in the proliferation of melanoma cells. In addition, miR-633 inhibitor transfection into both the A375 and B16 melanoma cell lines decreased migration rates in wound healing assays (Fig. 4C) and cell invasion in Transwell assays (Fig. 4D). Collectively, these findings demonstrated that miR-633 was able to promote the migration and proliferation of melanoma cells.
|
|
title
|
miR-633 enhances melanoma cell proliferation and migration
|
|
p
|
Finally, the biological importance of miR-633 was assessed in melanoma. Using a CCK-8 assay, it was found that transfection with the miR-633 inhibitor significantly suppressed the numbers of melanoma cells over time compared with cells transfected with NC (Fig. 4A and B), suggesting a role for this miRNA in the proliferation of melanoma cells. In addition, miR-633 inhibitor transfection into both the A375 and B16 melanoma cell lines decreased migration rates in wound healing assays (Fig. 4C) and cell invasion in Transwell assays (Fig. 4D). Collectively, these findings demonstrated that miR-633 was able to promote the migration and proliferation of melanoma cells.
|
|
sec
|
Discussion
In the present study, it was demonstrated that miR-633 was significantly upregulated in melanoma tissue compared with adjacent normal tissue from11patients with melanoma. In addition, a series of melanoma cell lines exhibited a significantly higher level of miR-633 expression compared with HeMn cells. In previous studies, miR-633 was shown to be a functionally important tumor-associated miRNA in lung and brain cancer (28,29). Its functional importance in melanoma, however, had yet to be properly elucidated. The present study used the TargetScan, StarBase and miRanda algorithms to predict 6 candidate miRNAs that specifically targeted KAI1.KAI1 was shown to have one potential complementary miR-633-binding site within its 3′-UTR. Experimental results demonstrated that over expression of miR-633 led to a significant reduction in the levels of KAI1 protein, and deregulated expression of miR-633 significantly altered the proliferation, migration and invasion of A375 and B16 melanoma cells. Taken together, the findings of the present study have provided, to the best of the authors' knowledge, the first evidence thatmiR-633 may function as a tumor promoter in human melanoma, mediated partly through targeting KAI1 expression.
A total of 3,657 mature human miRNAs have been identified to date (miRBase, 2019, http://www.mirbase.org). In prior studies, miRNAs identified from the integumentary system were successfully used as diagnostic and prognostic biomarkers in patients with melanoma (30,31). For example, miR-213 is a suppressor of malignant melanoma progression, thereby indicating that it may be a potentially viable therapeutic target or diagnostic biomarker in patients with this form of cancer (32). Previous studies indicated that deregulation of miR-21 was associated with pro-apoptotic effects in pancreatic and breast cancer cell lines (33). In melanoma, a previous study revealed that miR-205, miR-200 and members of the let-7 family(−125b, −146a, −155, −21, −25, −23a and −29b) were deregulated (34). Previous studies also suggested that abnormal miR-633 expression was associated with the prednisone response early in childhood acute lymphoblastic leukemia relapse (35). However, abnormal miR-633 expression has only been definitively identified in few types of tumors to date. The association between miR-633 and malignant melanoma has not been previously elucidated. The present study demonstrated that the expression levels of miR-633 were upregulated in melanoma tissues and cell lines compared with normal tissues and cells, respectively. Furthermore, the results obtained revealed that deregulation of miR-633 significantly altered the proliferation and migration of A375 and B16 melanoma cells. Therefore, miR-633 may serve as a novel biomarker for melanoma in the future.
KAI1, also known as CD82, is an important transcriptional regulator and tumor suppressor gene, and KAI1 mutations are commonly encountered in a range of tumor types (36). KAI1 regulates chromatin accessibility, in part via the E-cadherin pathway (37), and prior bioinformatics analyses have suggested that KAI1 mutations are associated with lung, bone and brain cancer prognoses, underscoring the relevance of this gene in oncogenic contexts (38). KAI1 may attenuate signaling to shut down metastatic colonization through attenuation of epidermal growth factor receptor signaling and inhibition of the Wnt signaling pathway. You et al (39) suggested that the KAI1 promoter may be regulated by the p53 and NME/NM23 nucleoside diphosphate kinase 1 genes. In pancreatic carcinoma, over expressing KAI1 attenuated the phosphorylation of SRC and STAT3, thereby inhibiting the expression of vascular endothelial growth factor C and limiting the activity of pancreatic carcinoma cells (40). KAI1 has also been shown to serve as a mediator of metabolic reprogramming in tumor cells. For example, KAI1 can suppress the progression of cancers of the digestive system via influencing invasion-associated protein metabolism (41). It also serves as an important tumor suppressor gene in the context of integumental tumors, with KAI1 inactivating mutations being a common feature in human melanoma (42). The ability of KAI1 to suppress oncogenesis is linked to its ability to inhibit the expression of AP-1, JUNB, poly (ADP-ribose) polymerase (PARP) and other oncogenes (43). Khan et al (44) reported that KAI1 is a key regulator of Toll-like receptor 9 (TLR9) trafficking and signaling. KAI1 modulates TLR9-dependent NF-κB nuclear translocation, which is critical for inflammatory cytokine production. A recent study demonstrated that KAI1 was significantly associated with poor survival and could act as an independent prognostic factor in human melanoma for both 5-year and 10-year survival rates (45). KAI1 deregulation has been observed in melanoma cell lines and tumor samples, and the over expression of this protein may significantly reduce melanoma progression (46). Furthermore, miR-203 inhibited frizzled-2 expression via KAI1 expression in human lung carcinoma cells, andKAI1expression may be a useful marker for metastatic, invasive cancer, and prognostic factor in tumors, such as lung cancer (47). However, the specific mechanisms governing KAI1 downregulation in the context of melanoma had yet to be clarified.
The present study aimed to investigate the role of miR-633 in melanoma through its potential interaction with KAI1, revealing that miR-633 may be an important regulator of malignant melanoma. The results showed that miR-633 was upregulated in melanoma cells and tumor tissue samples compared with their controls. In addition, miR-633 inhibition resulted in impaired migration and proliferation of A375 and B16 melanoma cells in vitro. Notably, the results of the dual-luciferase reporter assay demonstrated that miR-633 directly regulated the expression of KAI1. Therefore, the data obtained in the present study have provided important insights into the regulation of KAI1 by miRNAs in cancer cells. miR-633 may serve as a potential candidate for the diagnosis and treatment of human melanoma.
In conclusion, the present study has provided novel evidence to show that inhibition of miR-633 suppresses the proliferation and migration of the malignant melanoma cell lines A375 and B16, partly via regulation of KAI1.
|
|
title
|
Discussion
|
|
p
|
In the present study, it was demonstrated that miR-633 was significantly upregulated in melanoma tissue compared with adjacent normal tissue from11patients with melanoma. In addition, a series of melanoma cell lines exhibited a significantly higher level of miR-633 expression compared with HeMn cells. In previous studies, miR-633 was shown to be a functionally important tumor-associated miRNA in lung and brain cancer (28,29). Its functional importance in melanoma, however, had yet to be properly elucidated. The present study used the TargetScan, StarBase and miRanda algorithms to predict 6 candidate miRNAs that specifically targeted KAI1.KAI1 was shown to have one potential complementary miR-633-binding site within its 3′-UTR. Experimental results demonstrated that over expression of miR-633 led to a significant reduction in the levels of KAI1 protein, and deregulated expression of miR-633 significantly altered the proliferation, migration and invasion of A375 and B16 melanoma cells. Taken together, the findings of the present study have provided, to the best of the authors' knowledge, the first evidence thatmiR-633 may function as a tumor promoter in human melanoma, mediated partly through targeting KAI1 expression.
|
|
p
|
A total of 3,657 mature human miRNAs have been identified to date (miRBase, 2019, http://www.mirbase.org). In prior studies, miRNAs identified from the integumentary system were successfully used as diagnostic and prognostic biomarkers in patients with melanoma (30,31). For example, miR-213 is a suppressor of malignant melanoma progression, thereby indicating that it may be a potentially viable therapeutic target or diagnostic biomarker in patients with this form of cancer (32). Previous studies indicated that deregulation of miR-21 was associated with pro-apoptotic effects in pancreatic and breast cancer cell lines (33). In melanoma, a previous study revealed that miR-205, miR-200 and members of the let-7 family(−125b, −146a, −155, −21, −25, −23a and −29b) were deregulated (34). Previous studies also suggested that abnormal miR-633 expression was associated with the prednisone response early in childhood acute lymphoblastic leukemia relapse (35). However, abnormal miR-633 expression has only been definitively identified in few types of tumors to date. The association between miR-633 and malignant melanoma has not been previously elucidated. The present study demonstrated that the expression levels of miR-633 were upregulated in melanoma tissues and cell lines compared with normal tissues and cells, respectively. Furthermore, the results obtained revealed that deregulation of miR-633 significantly altered the proliferation and migration of A375 and B16 melanoma cells. Therefore, miR-633 may serve as a novel biomarker for melanoma in the future.
|
|
p
|
KAI1, also known as CD82, is an important transcriptional regulator and tumor suppressor gene, and KAI1 mutations are commonly encountered in a range of tumor types (36). KAI1 regulates chromatin accessibility, in part via the E-cadherin pathway (37), and prior bioinformatics analyses have suggested that KAI1 mutations are associated with lung, bone and brain cancer prognoses, underscoring the relevance of this gene in oncogenic contexts (38). KAI1 may attenuate signaling to shut down metastatic colonization through attenuation of epidermal growth factor receptor signaling and inhibition of the Wnt signaling pathway. You et al (39) suggested that the KAI1 promoter may be regulated by the p53 and NME/NM23 nucleoside diphosphate kinase 1 genes. In pancreatic carcinoma, over expressing KAI1 attenuated the phosphorylation of SRC and STAT3, thereby inhibiting the expression of vascular endothelial growth factor C and limiting the activity of pancreatic carcinoma cells (40). KAI1 has also been shown to serve as a mediator of metabolic reprogramming in tumor cells. For example, KAI1 can suppress the progression of cancers of the digestive system via influencing invasion-associated protein metabolism (41). It also serves as an important tumor suppressor gene in the context of integumental tumors, with KAI1 inactivating mutations being a common feature in human melanoma (42). The ability of KAI1 to suppress oncogenesis is linked to its ability to inhibit the expression of AP-1, JUNB, poly (ADP-ribose) polymerase (PARP) and other oncogenes (43). Khan et al (44) reported that KAI1 is a key regulator of Toll-like receptor 9 (TLR9) trafficking and signaling. KAI1 modulates TLR9-dependent NF-κB nuclear translocation, which is critical for inflammatory cytokine production. A recent study demonstrated that KAI1 was significantly associated with poor survival and could act as an independent prognostic factor in human melanoma for both 5-year and 10-year survival rates (45). KAI1 deregulation has been observed in melanoma cell lines and tumor samples, and the over expression of this protein may significantly reduce melanoma progression (46). Furthermore, miR-203 inhibited frizzled-2 expression via KAI1 expression in human lung carcinoma cells, andKAI1expression may be a useful marker for metastatic, invasive cancer, and prognostic factor in tumors, such as lung cancer (47). However, the specific mechanisms governing KAI1 downregulation in the context of melanoma had yet to be clarified.
|
|
p
|
The present study aimed to investigate the role of miR-633 in melanoma through its potential interaction with KAI1, revealing that miR-633 may be an important regulator of malignant melanoma. The results showed that miR-633 was upregulated in melanoma cells and tumor tissue samples compared with their controls. In addition, miR-633 inhibition resulted in impaired migration and proliferation of A375 and B16 melanoma cells in vitro. Notably, the results of the dual-luciferase reporter assay demonstrated that miR-633 directly regulated the expression of KAI1. Therefore, the data obtained in the present study have provided important insights into the regulation of KAI1 by miRNAs in cancer cells. miR-633 may serve as a potential candidate for the diagnosis and treatment of human melanoma.
|
|
p
|
In conclusion, the present study has provided novel evidence to show that inhibition of miR-633 suppresses the proliferation and migration of the malignant melanoma cell lines A375 and B16, partly via regulation of KAI1.
|
|
back
|
Acknowledgements
Not applicable.
Funding
No funding was received.
Availability of data and materials
All data generated or analyzed during the present study are included in this published article.
Authors' contributions
YL and ZW made substantial contributions to the conception and design of the study. Both authors collected the samples and clinical data, and contributed significantly to data analysis. ZW performed the experiments and drafted the initial manuscript. YL gave final approval of the version to be published. Both authors agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy and integrity of any part of the work were appropriately investigated and resolved. Both authors have read and approved the final manuscript.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of Cangzhou Central Hospital (Cangzhou, China; approval no. 201903501). Written informed consent was provided by patients and their families prior to the study start.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
|
|
ack
|
Acknowledgements
Not applicable.
|
|
title
|
Acknowledgements
|
|
p
|
Not applicable.
|
|
sec
|
Funding
No funding was received.
|
|
title
|
Funding
|
|
p
|
No funding was received.
|
|
sec
|
Availability of data and materials
All data generated or analyzed during the present study are included in this published article.
|
|
title
|
Availability of data and materials
|
|
p
|
All data generated or analyzed during the present study are included in this published article.
|
|
sec
|
Authors' contributions
YL and ZW made substantial contributions to the conception and design of the study. Both authors collected the samples and clinical data, and contributed significantly to data analysis. ZW performed the experiments and drafted the initial manuscript. YL gave final approval of the version to be published. Both authors agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy and integrity of any part of the work were appropriately investigated and resolved. Both authors have read and approved the final manuscript.
|
|
title
|
Authors' contributions
|
|
p
|
YL and ZW made substantial contributions to the conception and design of the study. Both authors collected the samples and clinical data, and contributed significantly to data analysis. ZW performed the experiments and drafted the initial manuscript. YL gave final approval of the version to be published. Both authors agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy and integrity of any part of the work were appropriately investigated and resolved. Both authors have read and approved the final manuscript.
|
|
sec
|
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of Cangzhou Central Hospital (Cangzhou, China; approval no. 201903501). Written informed consent was provided by patients and their families prior to the study start.
|
|
title
|
Ethics approval and consent to participate
|
|
p
|
The present study was approved by the Ethics Committee of Cangzhou Central Hospital (Cangzhou, China; approval no. 201903501). Written informed consent was provided by patients and their families prior to the study start.
|
|
sec
|
Patient consent for publication
Not applicable.
|
|
title
|
Patient consent for publication
|
|
p
|
Not applicable.
|
|
sec
|
Competing interests
The authors declare that they have no competing interests.
|
|
title
|
Competing interests
|
|
p
|
The authors declare that they have no competing interests.
|
|
figure
|
Figure 1. miRNAs predicted to regulate KAI1 expression in melanoma. (A) Venn diagram of prediction results from the TargetScan, miRanda and StarBase analyses. Six miRNAs were predicted in common by all three analysis tools. (B) Reverse transcription-quantitative PCR results for the expression levels of the six miRNAs in melanoma tumor and adjacent normal tissues. *P<0.05. miRNA, miRNA; KAI1, CD82 antigen.
|
|
label
|
Figure 1.
|
|
caption
|
miRNAs predicted to regulate KAI1 expression in melanoma. (A) Venn diagram of prediction results from the TargetScan, miRanda and StarBase analyses. Six miRNAs were predicted in common by all three analysis tools. (B) Reverse transcription-quantitative PCR results for the expression levels of the six miRNAs in melanoma tumor and adjacent normal tissues. *P<0.05. miRNA, miRNA; KAI1, CD82 antigen.
|
|
p
|
miRNAs predicted to regulate KAI1 expression in melanoma. (A) Venn diagram of prediction results from the TargetScan, miRanda and StarBase analyses. Six miRNAs were predicted in common by all three analysis tools. (B) Reverse transcription-quantitative PCR results for the expression levels of the six miRNAs in melanoma tumor and adjacent normal tissues. *P<0.05. miRNA, miRNA; KAI1, CD82 antigen.
|
|
figure
|
Figure 2. miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression. (A) The expression of miR-633 was assessed in HeMn and melanoma cell lines. (B) miR-633 expression levels and (C) KAI1 mRNA expression levels were evaluated in melanoma tumor and adjacent normal tissue samples from patients. (D) The expression of miR-633 was negatively correlated with that of KAI1 in melanoma tissues. *P<0.05 vs. HeMn. miR, microRNA; KAI1, CD82 antigen.
|
|
label
|
Figure 2.
|
|
caption
|
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression. (A) The expression of miR-633 was assessed in HeMn and melanoma cell lines. (B) miR-633 expression levels and (C) KAI1 mRNA expression levels were evaluated in melanoma tumor and adjacent normal tissue samples from patients. (D) The expression of miR-633 was negatively correlated with that of KAI1 in melanoma tissues. *P<0.05 vs. HeMn. miR, microRNA; KAI1, CD82 antigen.
|
|
p
|
miR-633 is upregulated in melanoma and is negatively correlated with KAI1 expression. (A) The expression of miR-633 was assessed in HeMn and melanoma cell lines. (B) miR-633 expression levels and (C) KAI1 mRNA expression levels were evaluated in melanoma tumor and adjacent normal tissue samples from patients. (D) The expression of miR-633 was negatively correlated with that of KAI1 in melanoma tissues. *P<0.05 vs. HeMn. miR, microRNA; KAI1, CD82 antigen.
|
|
figure
|
Figure 3. miR-633 suppresses KAI1 expression in melanoma cells. (A) The putative miR-633 binding site in the KAI1 3′-untranslated region. (B) miR-633 inhibition and (C) miR-633 overexpression were confirmed by reverse transcription-quantitative PCR in A375 and B16 cells, following transfection with miR-633 inhibitor or miR-633 mimics, respectively. (D) Luciferase activity was evaluated in B16 and (E) A375 cells, following co-transfection with KAI1 WT or KAI1 MUT constructs along with miR-633 mimics or controls. (F) KAI1 mRNA expression levels and (G) KAI1 protein expression levels were evaluated in B16 and A375 cells following miR-633 mimics or NC transfection. *P<0.05 vs. NC. miR, microRNA; KAI1, CD82 antigen; WT, wild type; MUT, mutant; NC, negative control.
|
|
label
|
Figure 3.
|
|
caption
|
miR-633 suppresses KAI1 expression in melanoma cells. (A) The putative miR-633 binding site in the KAI1 3′-untranslated region. (B) miR-633 inhibition and (C) miR-633 overexpression were confirmed by reverse transcription-quantitative PCR in A375 and B16 cells, following transfection with miR-633 inhibitor or miR-633 mimics, respectively. (D) Luciferase activity was evaluated in B16 and (E) A375 cells, following co-transfection with KAI1 WT or KAI1 MUT constructs along with miR-633 mimics or controls. (F) KAI1 mRNA expression levels and (G) KAI1 protein expression levels were evaluated in B16 and A375 cells following miR-633 mimics or NC transfection. *P<0.05 vs. NC. miR, microRNA; KAI1, CD82 antigen; WT, wild type; MUT, mutant; NC, negative control.
|
|
p
|
miR-633 suppresses KAI1 expression in melanoma cells. (A) The putative miR-633 binding site in the KAI1 3′-untranslated region. (B) miR-633 inhibition and (C) miR-633 overexpression were confirmed by reverse transcription-quantitative PCR in A375 and B16 cells, following transfection with miR-633 inhibitor or miR-633 mimics, respectively. (D) Luciferase activity was evaluated in B16 and (E) A375 cells, following co-transfection with KAI1 WT or KAI1 MUT constructs along with miR-633 mimics or controls. (F) KAI1 mRNA expression levels and (G) KAI1 protein expression levels were evaluated in B16 and A375 cells following miR-633 mimics or NC transfection. *P<0.05 vs. NC. miR, microRNA; KAI1, CD82 antigen; WT, wild type; MUT, mutant; NC, negative control.
|
|
figure
|
Figure 4. miR-633 regulates melanoma cell proliferation and migration. (A) The viability of B16 and (B) A375 cells was assessed following miR-633 inhibitor or NC transfection. (C) Wound healing activity was assessed for A375 and B16 cells following transfection with miR-633 inhibitor or NC (magnification, ×20). (D) Transwell invasion activity was evaluated in A375 and B16 cells following miR-633 inhibitor or NC transfection (magnification, ×20). *P<0.05 vs. NC. miR, microRNA; OD, optical density; NC, negative control.
|
|
label
|
Figure 4.
|
|
caption
|
miR-633 regulates melanoma cell proliferation and migration. (A) The viability of B16 and (B) A375 cells was assessed following miR-633 inhibitor or NC transfection. (C) Wound healing activity was assessed for A375 and B16 cells following transfection with miR-633 inhibitor or NC (magnification, ×20). (D) Transwell invasion activity was evaluated in A375 and B16 cells following miR-633 inhibitor or NC transfection (magnification, ×20). *P<0.05 vs. NC. miR, microRNA; OD, optical density; NC, negative control.
|
|
p
|
miR-633 regulates melanoma cell proliferation and migration. (A) The viability of B16 and (B) A375 cells was assessed following miR-633 inhibitor or NC transfection. (C) Wound healing activity was assessed for A375 and B16 cells following transfection with miR-633 inhibitor or NC (magnification, ×20). (D) Transwell invasion activity was evaluated in A375 and B16 cells following miR-633 inhibitor or NC transfection (magnification, ×20). *P<0.05 vs. NC. miR, microRNA; OD, optical density; NC, negative control.
|
|
table-wrap
|
Table I. Clinicopathological characteristics of patients with melanoma (n=10).
Characteristic Number of patients, n
Total 10
Age, years
<45 2
≥45 8
Sex
Male 7
Female 3
TNM classification
I+II 7
III+IV 3
Distant metastasis
No 9
Yes 1
TNM, tumor-metastasis-node.
|
|
label
|
Table I.
|
|
caption
|
Clinicopathological characteristics of patients with melanoma (n=10).
|
|
p
|
Clinicopathological characteristics of patients with melanoma (n=10).
|
|
table
|
Characteristic Number of patients, n
Total 10
Age, years
<45 2
≥45 8
Sex
Male 7
Female 3
TNM classification
I+II 7
III+IV 3
Distant metastasis
No 9
Yes 1
|
|
tr
|
Characteristic Number of patients, n
|
|
th
|
Characteristic
|
|
th
|
Number of patients, n
|
|
tr
|
Total 10
|
|
td
|
Total
|
|
td
|
10
|
|
tr
|
Age, years
|
|
td
|
Age, years
|
|
tr
|
<45 2
|
|
td
|
<45
|
|
td
|
2
|
|
tr
|
≥45 8
|
|
td
|
≥45
|
|
td
|
8
|
|
tr
|
Sex
|
|
td
|
Sex
|
|
tr
|
Male 7
|
|
td
|
Male
|
|
td
|
7
|
|
tr
|
Female 3
|
|
td
|
Female
|
|
td
|
3
|
|
tr
|
TNM classification
|
|
td
|
TNM classification
|
|
tr
|
I+II 7
|
|
td
|
I+II
|
|
td
|
7
|
|
tr
|
III+IV 3
|
|
td
|
III+IV
|
|
td
|
3
|
|
tr
|
Distant metastasis
|
|
td
|
Distant metastasis
|
|
tr
|
No 9
|
|
td
|
No
|
|
td
|
9
|
|
tr
|
Yes 1
|
|
td
|
Yes
|
|
td
|
1
|
|
table-wrap-foot
|
TNM, tumor-metastasis-node.
|
|
footnote
|
TNM, tumor-metastasis-node.
|
|
p
|
TNM, tumor-metastasis-node.
|
