PMC:7723248 / 26560-27974 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7723248","sourcedb":"PMC","sourceid":"7723248","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7723248","text":"Our results demonstrate that pH affects the stability of MS2 and Φ6 differently in droplets. MS2 survived equally well in acidic, pH-neutral, and basic droplets, whereas Φ6 survived best in pH-neutral droplets and decayed more in acidic or basic droplets. Previous studies have reported that viruses in bulk solutions are sensitive to pH [57, 58]. Both non-enveloped and enveloped viruses are generally more susceptible in acidic and basic solutions than in pH-neutral solutions [57]. At extreme pHs, viruses decay due to the denaturing of surface proteins and the hydrolysis of the viral genome [48, 59]. However, MS2 appears to be insensitive to pH. In a previous study, a moderate decay rate of ~0.5 log10 unit per day was observed for MS2 in bulk solutions at pH values of 4 and 10, and MS2 retained its viability when the solution was pH-neutral. The effect of pH on the viability of enveloped viruses is generally more noticeable than its effect on non-enveloped viruses [57], consistent with our observation of pronounced decay of Φ6 in acidic solutions across all RH levels. Besides the protein denaturing effect, the fusion of enveloped viruses’ membrane structure caused by extreme pH also leads to inactivation [48]. Low-pH treatment is widely used in monoclonal antibody purification processes to inactivate viruses because of its reliable performance (e.g., \u003e 4 log10 decay) on enveloped viruses [60].","tracks":[]}