PMC:7723248 / 20133-21554 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7723248","sourcedb":"PMC","sourceid":"7723248","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7723248","text":"Surfactant\nThe relative viability of MS2 in droplets with different surfactant concentrations is shown in Fig 5A and 5C. MS2 generally survived better when SDS was present in droplets, and relative viability increased with SDS concentration at 20% and 80% RH. MS2 incurred no decay in droplets containing 10 μg/mL SDS, whereas it at least lost 25% viability in droplets containing no SDS. The relationship between viability and SDS concentration differed at 50% RH, at which MS2 survived best in droplets with 1 μg/mL SDS, but decayed most in droplets containing 10 μg/mL SDS. As shown in Fig 5B and 5D, SDS did not significantly affect the viability of Φ6 in droplets at RHs of 20%. However, 10 μg/mL SDS induced a significantly higher inactivation of Φ6 at RHs of 50% and 80%; no viable Φ6 was recovered from droplets containing 10 μg/mL SDS at high RH after 1 h.\nFig 5 Concentration of bacteriophages (A) MS2 and (B) Φ6 in droplets with different initial surfactant concentration before (dark bars) and after (light bars) 1 h exposure to low, intermediate, and high RH (mean ± s.d. of triplicates). Relative viability of (C) MS2 and (D) Φ6 after 1 h exposure (lines show the mean of triplicates). The number of virions in droplets at the start of the exposure experiments was 105−106 PFU. The dark gray dashed line indicates the detection limit (104 PFU/mL) of plaque assay. ND indicates no viable virus was detected.","divisions":[{"label":"title","span":{"begin":0,"end":10}},{"label":"p","span":{"begin":11,"end":865}},{"label":"label","span":{"begin":866,"end":871}}],"tracks":[]}