PMC:7723248 / 18540-20131 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7723248","sourcedb":"PMC","sourceid":"7723248","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7723248","text":"Protein\nSimilar to salt, the effect of protein on MS2 was also RH-dependent (Fig 4A and 4C). The relative viability of MS2 decreased as the concentration of BSA increased in droplets at 20% RH. However, at RHs of 50% and 80%, the relative viability was higher in the presence of BSA. At 50% RH, the viability of MS2 was reduced by only 7% in droplets containing 100 μg/mL BSA after 1 h, a significantly lower loss than the \u003e80% reduction in droplets that did not contain any BSA. At 80% RH, there was no decay in droplets containing BSA, regardless of its concentration, suggesting that BSA has a protective effect on the viability of MS2.\nFig 4 Concentration of bacteriophages (A) MS2 and (B) Φ6 in droplets with different initial protein concentration before (dark bars) and after (light bars) 1 h exposure to low, intermediate, and high RH (mean ± s.d. of triplicates). Relative viability of (C) MS2 and (D) Φ6 after 1 h exposure (lines show the mean of triplicates). The number of virions in droplets at the start of the exposure experiments was 105−106 PFU. Similar to its effect on MS2, BSA protected Φ6 from inactivation in droplets at intermediate and high RHs (Fig 4B and 4D). At 20% RH, the relative viability of Φ6 was similar in droplets with and without BSA. However, at 50% RH, the relative viability of Φ6 was significantly higher in droplets containing 1000 μg/mL BSA than in droplets with 0 or 100 μg/mL BSA. At 80% RH, the presence of BSA, regardless of its concentration, reduced the decay of Φ6 in droplets, suggesting its protective effect on viruses in droplets again.","divisions":[{"label":"title","span":{"begin":0,"end":7}},{"label":"p","span":{"begin":8,"end":639}},{"label":"figure","span":{"begin":640,"end":1063}},{"label":"label","span":{"begin":640,"end":645}},{"label":"caption","span":{"begin":647,"end":1063}},{"label":"p","span":{"begin":647,"end":1063}}],"tracks":[]}