Id |
Subject |
Object |
Predicate |
Lexical cue |
T424 |
0-183 |
Sentence |
denotes |
Observation of agglutination of human erythrocytes by intact MERS-CoV particles led to the finding that Sia-binding activity of MERS-CoV is mediated by multivalent S1A (S1-NTD) [112]. |
T425 |
184-352 |
Sentence |
denotes |
One hundred thirty-five glycan structures were used for determination of receptor binding specificity of multivalent MERS-S1A-containing nanoparticles by glycan arrays. |
T426 |
353-479 |
Sentence |
denotes |
It appeared that MERS-S1A nanoparticles bind selectively to nonmodified Neu5Ac but not to Neu5Gc or (7,)9-O-acetylated Neu5Ac. |
T427 |
480-669 |
Sentence |
denotes |
No binding to Neu5Gc-terminated glycans was explained by steric hindrance of the extra hydroxyl group of Neu5Gc in the MERS- S1A hydrophobic pocket formed by F39, F101, I131 and I132 [148]. |
T428 |
670-867 |
Sentence |
denotes |
For sialyl linkage specificity, MERS-CoV S1A prefers α2,3 over α2,6 linkages, either short, sulfated, α2,3-linked mono-Sia LewisX or long, branched, α2,3-linked tri-Sia with four type 2 LN repeats. |
T429 |
868-1018 |
Sentence |
denotes |
Pretreatment of Calu-3 human airway cells with neuraminidase inhibited infection of MERS-CoV, indicating that Sia is necessary for MERS-CoV infection. |
T430 |
1019-1388 |
Sentence |
denotes |
Accordingly, multivalent MERS-CoV S1A-containing nanoparticles were shown to specifically bind to infection sites in individual hosts: dromedary camel nasal epithelial cells, human type II pneumocytes in the alveolar wall that contains an abundance of α2,3-sialyl type 2 LN and a small amount of α2,3-sialyl-LewisX [19], and pipistrelle bat intestinal epithelial cells. |
T431 |
1389-1581 |
Sentence |
denotes |
Removal of Sia from cell surface glycans by treatment with neuraminidase abolished binding of the nanoparticles, confirming that MERS-CoV S1A binds to infected cells via Sia recognition [108]. |
T432 |
1582-1648 |
Sentence |
denotes |
The cryo-EM structure of MERS-CoV S in complex with α2,3-SLN (pdb: |
T433 |
1649-1746 |
Sentence |
denotes |
6q06) showed Sia binding in a surface-exposed groove (domain A) of each monomer (Figure 9, left). |
T434 |
1747-1891 |
Sentence |
denotes |
Analysis of the structure indicated the five residues Q36, A92, I132, S133 and R307 directly anchoring Neu5Ac as shown in Figure 9, right [148]. |