| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T74 |
0-13 |
Sentence |
denotes |
RT-qPCR assay |
| T75 |
14-124 |
Sentence |
denotes |
RT-qPCR was performed using the One Step PrimeScript III RT-qPCR Mix (Takara Bio) as reported previously [14]. |
| T76 |
125-343 |
Sentence |
denotes |
The reaction mixture (total volume = 20 μL) contained 10 μL of 2× One Step PrimeScript III RT-qPCR Mix, 0.4 μL of ROX Reference Dye, 2 μL of 10× primer-probe mixture, 2 μL of RNA sample, and 5.6 μL of RNase-free water. |
| T77 |
344-411 |
Sentence |
denotes |
Two different sets of primer-probe mixture were used in this study. |
| T78 |
412-608 |
Sentence |
denotes |
The first set was developed by NIID (NIID-qPCR) and used with the final concentration of 500 nM, 700 nM, and 200 nM of the forward primer, reverse primer, and FAM-labeled probe, respectively [15]. |
| T79 |
609-775 |
Sentence |
denotes |
The second primer-probe set was reported by WHO for detection of E gene of SARS-CoV-2 and SARS (WHO-qPCR), and used 400 nM of each primer and 200 nM of the probe [9]. |
| T80 |
776-1038 |
Sentence |
denotes |
The NIID-qPCR reaction was performed using the StepOnePlus instrument (Applied Biosystems) with a thermal cycle program of 52°C for 5 min, 95°C for 10 sec, followed by 45 cycles of 95°C for 5 sec, and 60°C for 30 sec WHO-qPCR was performed with the same program. |
| T81 |
1039-1103 |
Sentence |
denotes |
Cut-off values were set at the threshold cycle (Ct) value of 40. |
| T82 |
1104-1245 |
Sentence |
denotes |
To quantify viral RNA, a standard curve was generated with 10-fold serial dilutions of synthesized standard RNA of the qPCR target sequences. |
