PMC:7668588 / 1428-2271 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7668588","sourcedb":"PMC","sourceid":"7668588","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7668588","text":"Rapid, accurate and simple diagnostic assay is required to provide an appropriate treatment and establish a surveillance system to prevent the spread of COVID-19. In this study, we developed the RT-LAMP assay using a portable platform equipped with a battery for detection of SARS-CoV-2 in clinical specimens. It could detect over 23.7 copies within 15 min. Since RNA extraction step is one of the main obstacles for rapid diagnosis, we also developed the RT-LAMP assay without this step, Direct RT-LAMP. This assay could detect over 203 copies of viral RNA in virus-spiked swab solution within 10.5 min. Since our RT-LAMP assay is a rapid, simple and highly sensitive diagnostic system for identifying COVID-19 case, it would be useful for the detection of SARS-CoV-2, especially in the field or the settings with insufficient infrastructure.","tracks":[{"project":"LitCovid-PD-HP","denotations":[{"id":"T2","span":{"begin":652,"end":668},"obj":"Phenotype"}],"attributes":[{"id":"A2","pred":"hp_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/HP_0041092"},{"subj":"T2","pred":"source","obj":"LitCovid-PD-HP"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"30","span":{"begin":276,"end":286},"obj":"Species"},{"id":"31","span":{"begin":758,"end":768},"obj":"Species"},{"id":"32","span":{"begin":153,"end":161},"obj":"Disease"},{"id":"33","span":{"begin":703,"end":711},"obj":"Disease"}],"attributes":[{"id":"A30","pred":"tao:has_database_id","subj":"30","obj":"Tax:2697049"},{"id":"A31","pred":"tao:has_database_id","subj":"31","obj":"Tax:2697049"},{"id":"A32","pred":"tao:has_database_id","subj":"32","obj":"MESH:C000657245"},{"id":"A33","pred":"tao:has_database_id","subj":"33","obj":"MESH:C000657245"},{"subj":"30","pred":"source","obj":"LitCovid-PubTator"},{"subj":"31","pred":"source","obj":"LitCovid-PubTator"},{"subj":"32","pred":"source","obj":"LitCovid-PubTator"},{"subj":"33","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T12","span":{"begin":0,"end":162},"obj":"Sentence"},{"id":"T13","span":{"begin":163,"end":309},"obj":"Sentence"},{"id":"T14","span":{"begin":310,"end":357},"obj":"Sentence"},{"id":"T15","span":{"begin":358,"end":504},"obj":"Sentence"},{"id":"T16","span":{"begin":505,"end":604},"obj":"Sentence"},{"id":"T17","span":{"begin":605,"end":843},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T12","pred":"source","obj":"LitCovid-sentences"},{"subj":"T13","pred":"source","obj":"LitCovid-sentences"},{"subj":"T14","pred":"source","obj":"LitCovid-sentences"},{"subj":"T15","pred":"source","obj":"LitCovid-sentences"},{"subj":"T16","pred":"source","obj":"LitCovid-sentences"},{"subj":"T17","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-HP","color":"#ec93c1","default":true},{"id":"LitCovid-PubTator","color":"#93dbec"},{"id":"LitCovid-sentences","color":"#ece393"}]}]}}