PMC:7668588 / 10796-11680 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7668588","sourcedb":"PMC","sourceid":"7668588","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7668588","text":"We designed two SARS-CoV-2 specific LAMP primer sets that targeted conserved sequences in the ORF1b region (name: ORF1b-1 and ORF1b-2) (Table 1). To examine the sensitivity of the RT-LAMP assay for SARS-CoV-2, serial 10-fold dilutions of in vitro synthesized RNA possessing the target sequence of Wuhan-Hu-1 strain were used as template. All (6/6) or 83% (5/6) of the reactions with 50 copies of RNA yielded positive results with ORF1b-1 (RT-LAMP/ORF1b-1) or ORF 1b-2 primer sets (RT-LAMP/ORF1b-2), respectively (Table 2). Additionally, RT-LAMP/ORF1b-1 could detect samples containing 5 RNA copies once in six replicates. Moreover, both RT-LAMP/ORF1b-1 and RT-LAMP/ORF1b-2 could detect 500 RNA copies within 8 min and 50 RNA copies within 11.8 min (Table 2). These results indicate that this RT-LAMP assay can be used as a rapid and sensitive test for the detection of SARS-CoV-2 RNA.","tracks":[{"project":"LitCovid-PubTator","denotations":[{"id":"171","span":{"begin":94,"end":99},"obj":"Gene"},{"id":"172","span":{"begin":36,"end":40},"obj":"Gene"},{"id":"173","span":{"begin":16,"end":26},"obj":"Species"},{"id":"174","span":{"begin":198,"end":208},"obj":"Species"},{"id":"175","span":{"begin":869,"end":879},"obj":"Species"}],"attributes":[{"id":"A171","pred":"tao:has_database_id","subj":"171","obj":"Gene:43740578"},{"id":"A172","pred":"tao:has_database_id","subj":"172","obj":"Gene:27074"},{"id":"A173","pred":"tao:has_database_id","subj":"173","obj":"Tax:2697049"},{"id":"A174","pred":"tao:has_database_id","subj":"174","obj":"Tax:2697049"},{"id":"A175","pred":"tao:has_database_id","subj":"175","obj":"Tax:2697049"},{"subj":"171","pred":"source","obj":"LitCovid-PubTator"},{"subj":"172","pred":"source","obj":"LitCovid-PubTator"},{"subj":"173","pred":"source","obj":"LitCovid-PubTator"},{"subj":"174","pred":"source","obj":"LitCovid-PubTator"},{"subj":"175","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T90","span":{"begin":0,"end":145},"obj":"Sentence"},{"id":"T91","span":{"begin":146,"end":337},"obj":"Sentence"},{"id":"T92","span":{"begin":338,"end":522},"obj":"Sentence"},{"id":"T93","span":{"begin":523,"end":621},"obj":"Sentence"},{"id":"T94","span":{"begin":622,"end":758},"obj":"Sentence"},{"id":"T95","span":{"begin":759,"end":884},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T90","pred":"source","obj":"LitCovid-sentences"},{"subj":"T91","pred":"source","obj":"LitCovid-sentences"},{"subj":"T92","pred":"source","obj":"LitCovid-sentences"},{"subj":"T93","pred":"source","obj":"LitCovid-sentences"},{"subj":"T94","pred":"source","obj":"LitCovid-sentences"},{"subj":"T95","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PubTator","color":"#bd93ec","default":true},{"id":"LitCovid-sentences","color":"#93eca3"}]}]}}