PMC:7640975 / 9280-10725
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T78 | 76-80 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T79 | 133-138 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T80 | 239-244 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T81 | 402-405 | Body_part | denotes | IgG | http://purl.org/sig/ont/fma/fma62872 |
| T82 | 450-455 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T83 | 519-524 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T84 | 606-613 | Body_part | denotes | Protein | http://purl.org/sig/ont/fma/fma67257 |
| T85 | 723-728 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T86 | 759-764 | Body_part | denotes | cells | http://purl.org/sig/ont/fma/fma68646 |
| T87 | 846-850 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T88 | 1017-1021 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T89 | 1186-1190 | Body_part | denotes | cell | http://purl.org/sig/ont/fma/fma68646 |
| T90 | 1402-1410 | Body_part | denotes | proteins | http://purl.org/sig/ont/fma/fma67257 |
| T91 | 1433-1440 | Body_part | denotes | protein | http://purl.org/sig/ont/fma/fma67257 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T143 | 72-80 | http://purl.obolibrary.org/obo/CLO_0008190 | denotes | per cell |
| T144 | 88-89 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T145 | 90-92 | http://purl.obolibrary.org/obo/CLO_0001382 | denotes | 48 |
| T146 | 133-138 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T147 | 239-244 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T148 | 387-391 | http://purl.obolibrary.org/obo/NCBITaxon_9925 | denotes | goat |
| T149 | 450-455 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T150 | 519-524 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T151 | 647-655 | http://purl.obolibrary.org/obo/CLO_0007225 | denotes | Labeling |
| T152 | 723-728 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T153 | 759-764 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
| T154 | 846-850 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T155 | 1017-1021 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T156 | 1186-1190 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T157 | 1335-1339 | http://purl.obolibrary.org/obo/CLO_0001387 | denotes | 4 °C |
LitCovid-PD-CHEBI
| Id | Subject | Object | Predicate | Lexical cue | chebi_id |
|---|---|---|---|---|---|
| T44 | 124-127 | Chemical | denotes | CO2 | http://purl.obolibrary.org/obo/CHEBI_16526 |
| T45 | 171-178 | Chemical | denotes | ethanol | http://purl.obolibrary.org/obo/CHEBI_16236 |
| T46 | 360-375 | Chemical | denotes | Alexa Fluor 488 | http://purl.obolibrary.org/obo/CHEBI_52661 |
| T47 | 366-371 | Chemical | denotes | Fluor | http://purl.obolibrary.org/obo/CHEBI_24061 |
| T48 | 606-613 | Chemical | denotes | Protein | http://purl.obolibrary.org/obo/CHEBI_16541 |
| T49 | 882-887 | Chemical | denotes | group | http://purl.obolibrary.org/obo/CHEBI_24433 |
| T50 | 1083-1089 | Chemical | denotes | buffer | http://purl.obolibrary.org/obo/CHEBI_35225 |
| T51 | 1132-1135 | Chemical | denotes | SDS | http://purl.obolibrary.org/obo/CHEBI_8984 |
| T52 | 1141-1145 | Chemical | denotes | urea | http://purl.obolibrary.org/obo/CHEBI_16199 |
| T53 | 1150-1169 | Chemical | denotes | protease inhibitors | http://purl.obolibrary.org/obo/CHEBI_37670|http://purl.obolibrary.org/obo/CHEBI_60258 |
| T55 | 1159-1169 | Chemical | denotes | inhibitors | http://purl.obolibrary.org/obo/CHEBI_35222 |
| T56 | 1402-1410 | Chemical | denotes | proteins | http://purl.obolibrary.org/obo/CHEBI_36080 |
| T57 | 1433-1440 | Chemical | denotes | protein | http://purl.obolibrary.org/obo/CHEBI_36080 |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T26 | 626-635 | http://purl.obolibrary.org/obo/GO_0007586 | denotes | Digestion |
| T27 | 1077-1082 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T46 | 82-210 | Sentence | denotes | After a 48-h cultivation at 37 °C with 5% CO2, the cells were fixed with prechilled 100% ethanol for 15 min at room temperature. |
| T47 | 211-309 | Sentence | denotes | After washing with PBS, the cells were probed with the mAb 1A3 (1:5000 dilution) at 37 °C for 1 h. |
| T48 | 310-487 | Sentence | denotes | Following another washing step, 1000-fold-diluted Alexa Fluor 488-conjugated goat antimouse IgG (Thermo Fisher Scientific) was added to the cells and incubated at 37 °C for 1 h. |
| T49 | 488-599 | Sentence | denotes | After washing thrice with PBS, cells were observed with an Eclipse Ci-S microscope (Nikon Corp., Tokyo, Japan). |
| T50 | 601-655 | Sentence | denotes | 2.5 Protein Extraction, Digestion, and iTRAQ Labeling |
| T51 | 656-860 | Sentence | denotes | At 24 h postinfection (hpi), both PDCoV- and mock-infected IPEC-J2 cells grown in T25 flasks (∼5 × 106 cells/flask) were rinsed twice with prechilled PBS, and then harvested with disposable cell scrapers. |
| T52 | 861-971 | Sentence | denotes | Three flasks of each group (PDCoV or mock) were harvested and used as three independent biological replicates. |
| T53 | 972-1181 | Sentence | denotes | After centrifugation at 300g for 10 min, the cell pellets from each flask were lysed with 800 μL of RIPA lysis buffer (Beyotime, Shanghai, China) containing 1% SDS, 8 M urea and protease inhibitors (Beyotime). |
| T54 | 1182-1287 | Sentence | denotes | The cell lysates were further sonicated on ice for 5 min with 10 s bursts and 10 s pauses between cycles. |
| T55 | 1288-1411 | Sentence | denotes | After centrifugation for 20 min at 12 000g and 4 °C, the supernatant was collected and used as the total cellular proteins. |
LitCovid-PubTator
| Id | Subject | Object | Predicate | Lexical cue | tao:has_database_id |
|---|---|---|---|---|---|
| 200 | 124-127 | Chemical | denotes | CO2 | MESH:D002245 |
| 201 | 171-178 | Chemical | denotes | ethanol | MESH:D000431 |
| 202 | 230-233 | Chemical | denotes | PBS | |
| 203 | 360-375 | Chemical | denotes | Alexa Fluor 488 | |
| 204 | 514-517 | Chemical | denotes | PBS | |
| 205 | 16-24 | Disease | denotes | infected | MESH:D007239 |
| 206 | 28-36 | Disease | denotes | infected | MESH:D007239 |
| 216 | 806-809 | Chemical | denotes | PBS | |
| 217 | 1072-1089 | Chemical | denotes | RIPA lysis buffer | |
| 218 | 1132-1135 | Chemical | denotes | SDS | MESH:D012967 |
| 219 | 1141-1145 | Chemical | denotes | urea | MESH:D014508 |
| 220 | 706-714 | Disease | denotes | infected | MESH:D007239 |
| 221 | 715-722 | CellLine | denotes | IPEC-J2 | CVCL:2246 |