PMC:7640975 / 6453-7822 JSONTXT

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Cells were cultured at 37 °C in a humidified CO2 incubator (Thermo Fisher Scientific, Waltham, MA, USA). The monoclonal antibody (mAb), 1A3, raised against the PDCoV nucleocapsid protein was prepared in our laboratory. Mouse anti-β-actin mAb, mouse anti-IFIT1 and rabbit anti-ANAPC7 polyclonal antibodies were bought from Abcam (Cambridge, MA, USA). Horseradish peroxidase (HRP)-conjugated goat antimouse/rabbit immunoglobulin G (IgG) were obtained from Sigma-Aldrich. Alexa Fluor 488-conjugated goat antimouse IgG was the product of Thermo Fisher 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