PMC:7640975 / 18641-18899 JSONTXT

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T240","span":{"begin":209,"end":210},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"tion volume comprised 10 μL of TaKaRa SYBR Premix Ex Taq, 0.4 μL of each forward and reverse primer (10 μM), 7.2 μL of nuclease-free water, and 2 μL of template. The qPCR amplifications were carried out using a thermal profile with an initial step at 95 °C f"}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T133","span":{"begin":133,"end":138},"obj":"Chemical"}],"attributes":[{"id":"A133","pred":"chebi_id","subj":"T133","obj":"http://purl.obolibrary.org/obo/CHEBI_15377"}],"text":"tion volume comprised 10 μL of TaKaRa SYBR Premix Ex Taq, 0.4 μL of each forward and reverse primer (10 μM), 7.2 μL of nuclease-free water, and 2 μL of template. The qPCR amplifications were carried out using a thermal profile with an initial step at 95 °C f"}

{"project":"LitCovid-PubTator","denotations":[{"id":"309","span":{"begin":133,"end":138},"obj":"Chemical"}],"attributes":[{"id":"A309","pred":"tao:has_database_id","subj":"309","obj":"MESH:D014867"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"tion volume comprised 10 μL of TaKaRa SYBR Premix Ex Taq, 0.4 μL of each forward and reverse primer (10 μM), 7.2 μL of nuclease-free water, and 2 μL of template. The qPCR amplifications were carried out using a thermal profile with an initial step at 95 °C f"}