> top > docs > PMC:7640975 > spans > 17368-19151 > annotations

PMC:7640975 / 17368-19151 JSONTXT

Annnotations TAB JSON ListView MergeView

LitCovid-PD-FMA-UBERON

Id Subject Object Predicate Lexical cue fma_id
T114 112-116 Body_part denotes mRNA http://purl.org/sig/ont/fma/fma67122
T115 235-238 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T116 280-285 Body_part denotes cells http://purl.org/sig/ont/fma/fma68646
T117 336-339 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T118 433-436 Body_part denotes RNA http://purl.org/sig/ont/fma/fma67095
T119 672-677 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T120 696-700 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402
T121 814-819 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T122 1143-1148 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T123 1615-1620 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T124 1760-1765 Body_part denotes actin http://purl.org/sig/ont/fma/fma67843
T125 1776-1780 Body_part denotes gene http://purl.org/sig/ont/fma/fma74402

LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T232 280-285 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T233 446-447 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T234 590-591 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T235 681-682 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T236 696-700 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene
T237 820-825 http://purl.obolibrary.org/obo/OGG_0000000002 denotes genes
T238 1261-1265 http://purl.obolibrary.org/obo/CLO_0001564 denotes a 20
T239 1261-1265 http://purl.obolibrary.org/obo/CLO_0050194 denotes a 20
T240 1482-1483 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T241 1776-1780 http://purl.obolibrary.org/obo/OGG_0000000002 denotes gene

LitCovid-PD-CHEBI

Id Subject Object Predicate Lexical cue chebi_id
T133 1406-1411 Chemical denotes water http://purl.obolibrary.org/obo/CHEBI_15377

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T32 85-100 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T33 476-485 http://purl.obolibrary.org/obo/GO_0009058 denotes Synthesis

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T91 0-38 Sentence denotes 2.9 Quantitative Real-Time PCR (qPCR)
T92 39-219 Sentence denotes To verify the DEPs identified by iTRAQ at the transcriptional level, the mRNA of two representative DEPs, the downregulated ANAPC7 and the upregulated IFIT1, were detected by qPCR.
T93 220-371 Sentence denotes Total cellular RNA of both PDCoV- and mock-infected IPEC-J2 cells was extracted using the TaKaRa MiniBEST universal RNA extraction kit (Dalian, China).
T94 372-552 Sentence denotes The first strand cDNA was synthesized using 2 μg of cellular RNA and with a RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) following the manufacturer’s instructions.
T95 553-701 Sentence denotes The qPCR assays were conducted using a TaKaRa SYBR Premix Ex Taq kit on an ABI 7500 real-time PCR machine along with β-actin as a housekeeping gene.
T96 702-906 Sentence denotes The primers used for the qPCR assay were designed based on the NCBI reference sequences for ANAPC7, IFIT1 and β-actin genes under GenBank accession numbers NM_016238, HQ679904 and NM_001101, respectively.
T97 907-982 Sentence denotes The information for the designed primers was as following: ANAPC7 (Forward:
T98 983-1019 Sentence denotes 5′-CTTTGCTGAGGAACGCACTG-3′, Reverse:
T99 1020-1064 Sentence denotes 5′-TCCATGTCGTCCACATCCTC-3′); IFIT1 (Forward:
T100 1065-1106 Sentence denotes 5′-GAAGATTTAACCCAACAAGAACATA-3′, Reverse:
T101 1107-1158 Sentence denotes 5′-CTTTCGATACGTAAGGTAATACAGC-3′); β-actin (Forward:
T102 1159-1195 Sentence denotes 5′-TCCCTGGAGAAGAGCTACGA-3′, Reverse:
T103 1196-1224 Sentence denotes 5′-AGCACTGTGTTGGCGTACAG-3′).
T104 1225-1434 Sentence denotes The qPCR assays were carried out in a 20-μL reaction volume comprised 10 μL of TaKaRa SYBR Premix Ex Taq, 0.4 μL of each forward and reverse primer (10 μM), 7.2 μL of nuclease-free water, and 2 μL of template.
T105 1435-1671 Sentence denotes The qPCR amplifications were carried out using a thermal profile with an initial step at 95 °C for 2 min, followed by 40 cycles of 95 °C for 10 s, 60 °C for 10 s (for ANAPC7 and β-actin) or 55 °C for 10 s (for IFIT1) and 72 °C for 40 s.
T106 1672-1783 Sentence denotes Fold-change values were calculated in term of the 2–ΔΔCt method and normalized to the β-actin reference gene.33

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
293 163-169 Gene denotes ANAPC7 Gene:51434
294 190-195 Gene denotes IFIT1 Gene:3434
295 670-677 Gene denotes β-actin Gene:728378
296 794-800 Gene denotes ANAPC7 Gene:51434
297 802-807 Gene denotes IFIT1 Gene:3434
298 812-819 Gene denotes β-actin Gene:728378
299 966-972 Gene denotes ANAPC7 Gene:51434
300 1049-1054 Gene denotes IFIT1 Gene:3434
301 1141-1148 Gene denotes β-actin Gene:728378
302 1602-1608 Gene denotes ANAPC7 Gene:51434
303 1613-1620 Gene denotes β-actin Gene:728378
304 1645-1650 Gene denotes IFIT1 Gene:3434
305 486-489 Gene denotes Kit Gene:396810
306 618-621 Gene denotes kit Gene:396810
307 351-354 Gene denotes kit Gene:396810
308 1758-1765 Gene denotes β-actin Gene:728378
309 1406-1411 Chemical denotes water MESH:D014867
310 263-271 Disease denotes infected MESH:D007239
311 272-279 CellLine denotes IPEC-J2 CVCL:2246