PMC:7594251 / 44864-45840 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7594251","sourcedb":"PMC","sourceid":"7594251","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7594251","text":"Saturation Transfer Difference (STD) NMR depends on the Nuclear Overhauser Effect (NOE), which is often used to enhance the sensitivity of less sensitive nuclei such as 13C and 15N [256,257]. This increase in sensitivity is possible because of dipolar coupling (i.e., through space interactions of separate nuclei) [257]. The increase in sensitivity is actually brought about by applying a long, low power radiofrequency pulse that selectively saturates the magnetization [256] of a specific chemical group (i.e., the methyl groups on a protein), which is then given time to transfer to another chemical group via the NOE dipolar coupling within a few angstroms [258]. The transfer in magnetization is easily visualized on a NMR spectrum that takes the differences in the signal intensities from before and after the irradiation. This new spectrum is called a “difference spectrum”, and it reveals what chemical groups interact with the irradiated signal [259] (see Figure 8).","tracks":[{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T80711","span":{"begin":537,"end":544},"obj":"Body_part"}],"attributes":[{"id":"A91418","pred":"fma_id","subj":"T80711","obj":"http://purl.org/sig/ont/fma/fma67257"},{"subj":"T80711","pred":"source","obj":"LitCovid-PD-FMA-UBERON"}]},{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T36","span":{"begin":32,"end":35},"obj":"Disease"}],"attributes":[{"id":"A36","pred":"mondo_id","subj":"T36","obj":"http://purl.obolibrary.org/obo/MONDO_0021681"},{"subj":"T36","pred":"source","obj":"LitCovid-PD-MONDO"}]},{"project":"LitCovid-PD-CLO","denotations":[{"id":"T306","span":{"begin":388,"end":389},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T307","span":{"begin":481,"end":482},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T308","span":{"begin":535,"end":536},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T309","span":{"begin":646,"end":647},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T310","span":{"begin":723,"end":724},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T311","span":{"begin":772,"end":778},"obj":"http://purl.obolibrary.org/obo/SO_0000418"},{"id":"T312","span":{"begin":851,"end":859},"obj":"http://purl.obolibrary.org/obo/PR_000001898"},{"id":"T313","span":{"begin":948,"end":954},"obj":"http://purl.obolibrary.org/obo/SO_0000418"}],"attributes":[{"subj":"T306","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T307","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T308","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T309","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T310","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T311","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T312","pred":"source","obj":"LitCovid-PD-CLO"},{"subj":"T313","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T436","span":{"begin":154,"end":160},"obj":"Chemical"},{"id":"T437","span":{"begin":307,"end":313},"obj":"Chemical"},{"id":"T438","span":{"begin":501,"end":506},"obj":"Chemical"},{"id":"T439","span":{"begin":518,"end":524},"obj":"Chemical"},{"id":"T441","span":{"begin":537,"end":544},"obj":"Chemical"},{"id":"T442","span":{"begin":604,"end":609},"obj":"Chemical"}],"attributes":[{"id":"A436","pred":"chebi_id","subj":"T436","obj":"http://purl.obolibrary.org/obo/CHEBI_33252"},{"id":"A437","pred":"chebi_id","subj":"T437","obj":"http://purl.obolibrary.org/obo/CHEBI_33252"},{"id":"A438","pred":"chebi_id","subj":"T438","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"id":"A439","pred":"chebi_id","subj":"T439","obj":"http://purl.obolibrary.org/obo/CHEBI_32875"},{"id":"A440","pred":"chebi_id","subj":"T439","obj":"http://purl.obolibrary.org/obo/CHEBI_29309"},{"id":"A441","pred":"chebi_id","subj":"T441","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A442","pred":"chebi_id","subj":"T442","obj":"http://purl.obolibrary.org/obo/CHEBI_24433"},{"subj":"T436","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T437","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T438","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T439","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T441","pred":"source","obj":"LitCovid-PD-CHEBI"},{"subj":"T442","pred":"source","obj":"LitCovid-PD-CHEBI"}]},{"project":"LitCovid-PubTator","denotations":[{"id":"582","span":{"begin":169,"end":172},"obj":"Chemical"},{"id":"583","span":{"begin":177,"end":180},"obj":"Chemical"}],"attributes":[{"id":"A582","pred":"tao:has_database_id","subj":"582","obj":"MESH:C000615229"},{"subj":"582","pred":"source","obj":"LitCovid-PubTator"},{"subj":"583","pred":"source","obj":"LitCovid-PubTator"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T315","span":{"begin":0,"end":191},"obj":"Sentence"},{"id":"T316","span":{"begin":192,"end":321},"obj":"Sentence"},{"id":"T317","span":{"begin":322,"end":668},"obj":"Sentence"},{"id":"T318","span":{"begin":669,"end":829},"obj":"Sentence"},{"id":"T319","span":{"begin":830,"end":976},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T315","pred":"source","obj":"LitCovid-sentences"},{"subj":"T316","pred":"source","obj":"LitCovid-sentences"},{"subj":"T317","pred":"source","obj":"LitCovid-sentences"},{"subj":"T318","pred":"source","obj":"LitCovid-sentences"},{"subj":"T319","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute 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