PMC:7574920 / 57310-57780 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T197","span":{"begin":299,"end":302},"obj":"Body_part"}],"attributes":[{"id":"A197","pred":"fma_id","subj":"T197","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T462","span":{"begin":4,"end":5},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T89454","span":{"begin":299,"end":302},"obj":"Chemical"}],"attributes":[{"id":"A59274","pred":"chebi_id","subj":"T89454","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}

{"project":"LitCovid-PubTator","denotations":[{"id":"395","span":{"begin":281,"end":284},"obj":"Gene"}],"attributes":[{"id":"A395","pred":"tao:has_database_id","subj":"395","obj":"Gene:5711"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T301","span":{"begin":317,"end":319},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T302","span":{"begin":371,"end":373},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}

{"project":"LitCovid-sentences","denotations":[{"id":"T412","span":{"begin":0,"end":146},"obj":"Sentence"},{"id":"T413","span":{"begin":147,"end":325},"obj":"Sentence"},{"id":"T414","span":{"begin":326,"end":470},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For a few samples, we saw so few reads (less than 200 UMIs) that we suspected that the multiplexing had failed and excluded them from the results. As most of these were in the same row of the same plate, we analyzed these samples after LAMP-sequencing by gel electrophoresis (fig. S5B) to check for DNA content after RT-LAMP. We found that the gel results agree with the RT-LAMP outcome, indicating that the failure likely was caused later, probably during multiplexing."}