PMC:7574920 / 54306-54700 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/7574920","sourcedb":"PMC","sourceid":"7574920","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7574920","text":"One PCR per plate with 1 μl of the eluate and RT-LAMP–specific and Tn5-adapter–specific primers (P7nxt-GeneN-A-LBrc and P7-xi7001..7016, P5.fw) was performed using NEBNext Q5 HotStart polymerase (New England Biolabs) with two cycles at 62°C for annealing and 90 s elongation, followed by two cycles at 65°C for annealing and 90 s elongation, and 13 cycles at 72°C annealing and 90 s elongation.","tracks":[{"project":"LitCovid-PD-CLO","denotations":[{"id":"T438","span":{"begin":109,"end":110},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"attributes":[{"subj":"T438","pred":"source","obj":"LitCovid-PD-CLO"}]},{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T298","span":{"begin":46,"end":48},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"attributes":[{"subj":"T298","pred":"source","obj":"LitCovid-PD-GO-BP"}]},{"project":"LitCovid-PD-GlycoEpitope","denotations":[{"id":"T7","span":{"begin":67,"end":70},"obj":"GlycoEpitope"}],"attributes":[{"id":"A7","pred":"glyco_epitope_db_id","subj":"T7","obj":"http://www.glycoepitope.jp/epitopes/AN0083"},{"subj":"T7","pred":"source","obj":"LitCovid-PD-GlycoEpitope"}]},{"project":"LitCovid-sentences","denotations":[{"id":"T393","span":{"begin":0,"end":394},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T393","pred":"source","obj":"LitCovid-sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCovid-PD-CLO","color":"#93ece8","default":true},{"id":"LitCovid-PD-GO-BP","color":"#ecce93"},{"id":"LitCovid-PD-GlycoEpitope","color":"#b493ec"},{"id":"LitCovid-sentences","color":"#93ec9a"}]}]}}