PMC:7574920 / 53325-53669 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T191","span":{"begin":78,"end":81},"obj":"Body_part"}],"attributes":[{"id":"A191","pred":"fma_id","subj":"T191","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T433","span":{"begin":219,"end":223},"obj":"http://purl.obolibrary.org/obo/PR_000002979"},{"id":"T434","span":{"begin":287,"end":288},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T435","span":{"begin":336,"end":342},"obj":"http://purl.obolibrary.org/obo/CLO_0009382"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T47167","span":{"begin":78,"end":81},"obj":"Chemical"},{"id":"T6045","span":{"begin":150,"end":156},"obj":"Chemical"},{"id":"T74345","span":{"begin":165,"end":169},"obj":"Chemical"},{"id":"T27218","span":{"begin":170,"end":183},"obj":"Chemical"},{"id":"T25862","span":{"begin":184,"end":195},"obj":"Chemical"},{"id":"T6351","span":{"begin":212,"end":216},"obj":"Chemical"},{"id":"T29057","span":{"begin":241,"end":246},"obj":"Chemical"}],"attributes":[{"id":"A46006","pred":"chebi_id","subj":"T47167","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"},{"id":"A67988","pred":"chebi_id","subj":"T6045","obj":"http://purl.obolibrary.org/obo/CHEBI_35225"},{"id":"A16149","pred":"chebi_id","subj":"T74345","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A52030","pred":"chebi_id","subj":"T27218","obj":"http://purl.obolibrary.org/obo/CHEBI_24712"},{"id":"A28722","pred":"chebi_id","subj":"T25862","obj":"http://purl.obolibrary.org/obo/CHEBI_44209"},{"id":"A92524","pred":"chebi_id","subj":"T6351","obj":"http://purl.obolibrary.org/obo/CHEBI_37527"},{"id":"A89507","pred":"chebi_id","subj":"T29057","obj":"http://purl.obolibrary.org/obo/CHEBI_6636"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}

{"project":"LitCovid-PubTator","denotations":[{"id":"383","span":{"begin":219,"end":223},"obj":"Disease"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T297","span":{"begin":53,"end":55},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}

{"project":"LitCovid-sentences","denotations":[{"id":"T387","span":{"begin":0,"end":344},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Tagmentation was carried out by mixing 1.2 μl of the RT-LAMP product (~200 ng DNA) with 1.5 μl of loaded transposase in freshly prepared tagmentation buffer [10 mM [tris(hydroxymethyl)methylamino]propanesulfonic acid) (TAPS)] (pH 8.5), 5 mM MgCl2, and 10% (v/v) dimethylformamide] using a Liquidator 96 Manual Pipetting System (Mettler Toledo)."}