PMC:7574920 / 53112-53324 JSONTXT

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T432","span":{"begin":102,"end":104},"obj":"http://purl.obolibrary.org/obo/CLO_0053794"}],"text":"Transposons were assembled by mixing Tn5(E54K, L372P) transposase (100 ng/μl) [purified according to (41)] with 1.25 μM annealed adapters in 50 mM Tris-HCl (pH 7.5) and incubating the reaction for 1 hour at 23°C."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T99414","span":{"begin":147,"end":151},"obj":"Chemical"},{"id":"T12523","span":{"begin":152,"end":155},"obj":"Chemical"}],"attributes":[{"id":"A59004","pred":"chebi_id","subj":"T99414","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A38373","pred":"chebi_id","subj":"T12523","obj":"http://purl.obolibrary.org/obo/CHEBI_17883"}],"text":"Transposons were assembled by mixing Tn5(E54K, L372P) transposase (100 ng/μl) [purified according to (41)] with 1.25 μM annealed adapters in 50 mM Tris-HCl (pH 7.5) and incubating the reaction for 1 hour at 23°C."}

{"project":"LitCovid-PubTator","denotations":[{"id":"373","span":{"begin":0,"end":11},"obj":"Species"},{"id":"384","span":{"begin":41,"end":45},"obj":"Mutation"},{"id":"385","span":{"begin":47,"end":52},"obj":"Mutation"}],"attributes":[{"id":"A373","pred":"tao:has_database_id","subj":"373","obj":"Tax:2387"},{"id":"A384","pred":"tao:has_standard_notation","subj":"384","obj":"p.E54K"},{"id":"A385","pred":"tao:has_standard_notation","subj":"385","obj":"p.L372P"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Transposons were assembled by mixing Tn5(E54K, L372P) transposase (100 ng/μl) [purified according to (41)] with 1.25 μM annealed adapters in 50 mM Tris-HCl (pH 7.5) and incubating the reaction for 1 hour at 23°C."}

{"project":"LitCovid-PD-GlycoEpitope","denotations":[{"id":"T6","span":{"begin":37,"end":40},"obj":"GlycoEpitope"}],"attributes":[{"id":"A6","pred":"glyco_epitope_db_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/AN0083"}],"text":"Transposons were assembled by mixing Tn5(E54K, L372P) transposase (100 ng/μl) [purified according to (41)] with 1.25 μM annealed adapters in 50 mM Tris-HCl (pH 7.5) and incubating the reaction for 1 hour at 23°C."}

{"project":"LitCovid-sentences","denotations":[{"id":"T386","span":{"begin":0,"end":212},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transposons were assembled by mixing Tn5(E54K, L372P) transposase (100 ng/μl) [purified according to (41)] with 1.25 μM annealed adapters in 50 mM Tris-HCl (pH 7.5) and incubating the reaction for 1 hour at 23°C."}