PMC:7574920 / 52804-53111 JSONTXT

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T431","span":{"begin":292,"end":293},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"In detail, transposon adapters containing well-defining barcodes and unique molecular identifiers (UMIs) were annealed by mixing 25 μM oligos (P5-UMI-xi5001…5096-ME.fw, Tn5hY-Rd2-Wat-SC3) in 5 μM tris-HCl (pH 8), incubating at 99°C for 5 min, and slowly cooling down to 20°C within 15 min in a thermocycler."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T75557","span":{"begin":196,"end":200},"obj":"Chemical"},{"id":"T83003","span":{"begin":201,"end":204},"obj":"Chemical"}],"attributes":[{"id":"A70175","pred":"chebi_id","subj":"T75557","obj":"http://purl.obolibrary.org/obo/CHEBI_9754"},{"id":"A61348","pred":"chebi_id","subj":"T83003","obj":"http://purl.obolibrary.org/obo/CHEBI_17883"}],"text":"In detail, transposon adapters containing well-defining barcodes and unique molecular identifiers (UMIs) were annealed by mixing 25 μM oligos (P5-UMI-xi5001…5096-ME.fw, Tn5hY-Rd2-Wat-SC3) in 5 μM tris-HCl (pH 8), incubating at 99°C for 5 min, and slowly cooling down to 20°C within 15 min in a thermocycler."}

{"project":"LitCovid-PubTator","denotations":[{"id":"372","span":{"begin":206,"end":208},"obj":"Gene"},{"id":"374","span":{"begin":196,"end":200},"obj":"Chemical"},{"id":"375","span":{"begin":201,"end":204},"obj":"Chemical"}],"attributes":[{"id":"A372","pred":"tao:has_database_id","subj":"372","obj":"Gene:5053"},{"id":"A375","pred":"tao:has_database_id","subj":"375","obj":"MESH:D006851"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In detail, transposon adapters containing well-defining barcodes and unique molecular identifiers (UMIs) were annealed by mixing 25 μM oligos (P5-UMI-xi5001…5096-ME.fw, Tn5hY-Rd2-Wat-SC3) in 5 μM tris-HCl (pH 8), incubating at 99°C for 5 min, and slowly cooling down to 20°C within 15 min in a thermocycler."}

{"project":"LitCovid-sentences","denotations":[{"id":"T385","span":{"begin":0,"end":307},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In detail, transposon adapters containing well-defining barcodes and unique molecular identifiers (UMIs) were annealed by mixing 25 μM oligos (P5-UMI-xi5001…5096-ME.fw, Tn5hY-Rd2-Wat-SC3) in 5 μM tris-HCl (pH 8), incubating at 99°C for 5 min, and slowly cooling down to 20°C within 15 min in a thermocycler."}