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PMC:7574920 / 51259-52509 JSONTXT

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LitCovid-PD-CLO

Id Subject Object Predicate Lexical cue
T422 94-95 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T423 221-222 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T424 304-305 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T425 445-446 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T426 515-516 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T427 612-615 http://purl.obolibrary.org/obo/CLO_0001387 denotes 4°C
T428 719-720 http://purl.obolibrary.org/obo/CLO_0001020 denotes a
T429 1031-1032 http://purl.obolibrary.org/obo/CLO_0001020 denotes a

LitCovid-PubTator

Id Subject Object Predicate Lexical cue tao:has_database_id
352 764-767 Gene denotes mix Gene:83881
353 205-208 Gene denotes mix Gene:83881
354 43-50 Species denotes patient Tax:9606
355 680-687 Species denotes patient Tax:9606
356 383-388 Chemical denotes metal MESH:D008670
357 1229-1234 Chemical denotes metal MESH:D008670

LitCovid-PD-GO-BP

Id Subject Object Predicate Lexical cue
T293 27-29 http://purl.obolibrary.org/obo/GO_0001171 denotes RT
T294 955-957 http://purl.obolibrary.org/obo/GO_0001171 denotes RT

LitCovid-sentences

Id Subject Object Predicate Lexical cue
T372 0-115 Sentence denotes For direct and hot swab–to–RT-LAMP assays, patient swab specimens were transferred first onto a 96-well seed plate.
T373 116-276 Sentence denotes For the direct assay, we then transferred 1 μl of the specimen directly to 19 μl of LAMP mix per well in a ready-made 96-well PCR plate (0030128672, Eppendorf).
T374 277-395 Sentence denotes The plate was sealed using a transparent adhesive foil (GK480-OS, Kisker Biotech) and kept on an ice-cold metal block.
T375 396-577 Sentence denotes For the hot assay, we sealed the seed plate with a pierceable lid (4ti-0566/96, Brooks Life Sciences) and heated it in a PCR cycler for 5 min at 95°C (with the lid heated to 105°C).
T376 578-643 Sentence denotes The seed plate was cooled down to 4°C on an ice-cold metal block.
T377 644-777 Sentence denotes Afterward, 1 μl of the heat-treated patient specimens was quickly added to a second ready-made plate with 19 μl of LAMP mix per well.
T378 778-863 Sentence denotes This plate was also sealed with transparent adhesive foil (GK480-OS, Kisker Biotech).
T379 864-937 Sentence denotes Both plates were then incubated at 65°C for the LAMP reaction to proceed.
T380 938-1099 Sentence denotes For both swab–to–RT-LAMP assays, the PCR plates were briefly spun down and then incubated in a PCR cycler at 65°C for 10 to 60 min (with the lid heated to 75°C).
T381 1100-1250 Sentence denotes To perform measurements at the indicated time points, the reactions were taken out of the PCR cycler and placed into an ice-cold metal block for 30 s.