PMC:7574920 / 46951-47230 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T177","span":{"begin":84,"end":87},"obj":"Body_part"},{"id":"T178","span":{"begin":188,"end":192},"obj":"Body_part"},{"id":"T179","span":{"begin":224,"end":228},"obj":"Body_part"},{"id":"T180","span":{"begin":229,"end":232},"obj":"Body_part"}],"attributes":[{"id":"A177","pred":"fma_id","subj":"T177","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A178","pred":"fma_id","subj":"T178","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A179","pred":"fma_id","subj":"T179","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A180","pred":"fma_id","subj":"T180","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T393","span":{"begin":159,"end":160},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T394","span":{"begin":188,"end":192},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T395","span":{"begin":194,"end":195},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T396","span":{"begin":224,"end":228},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T397","span":{"begin":268,"end":269},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T92","span":{"begin":209,"end":218},"obj":"Chemical"}],"attributes":[{"id":"A92","pred":"chebi_id","subj":"T92","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}

{"project":"LitCovid-PubTator","denotations":[{"id":"296","span":{"begin":0,"end":3},"obj":"Gene"}],"attributes":[{"id":"A296","pred":"tao:has_database_id","subj":"296","obj":"Gene:83881"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T275","span":{"begin":131,"end":133},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T276","span":{"begin":237,"end":239},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}

{"project":"LitCovid-sentences","denotations":[{"id":"T341","span":{"begin":108,"end":193},"obj":"Sentence"},{"id":"T342","span":{"begin":194,"end":278},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"mix (10 μl) was distributed per reaction into 96-well plates, and 10 μl of purified RNA was added per well. The performance of the RT-qPCR was validated using a positive control for the E gene. A total of 103 molecules of E gene RNA per RT-qPCR reaction correspond to a CT ≈ 30.\n"}