PMC:7574920 / 35531-36310
Annnotations
LitCovid-PD-FMA-UBERON
| Id | Subject | Object | Predicate | Lexical cue | fma_id |
|---|---|---|---|---|---|
| T140 | 138-141 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T141 | 397-400 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
| T142 | 535-538 | Body_part | denotes | RNA | http://purl.org/sig/ont/fma/fma67095 |
LitCovid-PD-CLO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T305 | 30-31 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T306 | 457-458 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T307 | 475-479 | http://purl.obolibrary.org/obo/UBERON_0000473 | denotes | test |
| T308 | 651-652 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
| T309 | 716-717 | http://purl.obolibrary.org/obo/CLO_0001020 | denotes | a |
LitCovid-PD-GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T215 | 232-234 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T216 | 467-469 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T217 | 631-633 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
| T218 | 695-697 | http://purl.obolibrary.org/obo/GO_0001171 | denotes | RT |
LitCovid-sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T267 | 0-255 | Sentence | denotes | Last, our analysis found that a short heat treatment of 5 min at 95°C, which poses minimal additional handling steps, did not destroy the RNA but rather stabilized it and this improved the sensitivity and specificity of the swab–to–RT-LAMP assay (Fig. 5). |
| T268 | 256-401 | Sentence | denotes | The heat likely helped to homogenize the sample, to inactivate ribonucleases (RNAses), and to break up the viral capsid to release the viral RNA. |
| T269 | 402-599 | Sentence | denotes | Overall, our data demonstrate the feasibility of using a swab–to–RT-LAMP test and suggest applications especially in scenarios where RNA isolation is not available, e.g., in resource-poor settings. |
| T270 | 600-779 | Sentence | denotes | In such cases, the hot swab–to–RT-LAMP assay seems a good option given that the direct swab–to–RT-LAMP assay yields a number of false positives due to spurious amplification (14). |