PMC:7574920 / 32602-33059 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T126","span":{"begin":217,"end":220},"obj":"Body_part"}],"attributes":[{"id":"A126","pred":"fma_id","subj":"T126","obj":"http://purl.org/sig/ont/fma/fma74412"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T77","span":{"begin":85,"end":93},"obj":"Disease"},{"id":"T78","span":{"begin":96,"end":105},"obj":"Disease"}],"attributes":[{"id":"A77","pred":"mondo_id","subj":"T77","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A78","pred":"mondo_id","subj":"T78","obj":"http://purl.obolibrary.org/obo/MONDO_0005550"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T280","span":{"begin":144,"end":145},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T281","span":{"begin":310,"end":311},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T282","span":{"begin":411,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T283","span":{"begin":424,"end":431},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T67","span":{"begin":217,"end":220},"obj":"Chemical"}],"attributes":[{"id":"A67","pred":"chebi_id","subj":"T67","obj":"http://purl.obolibrary.org/obo/CHEBI_16991"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-PubTator","denotations":[{"id":"232","span":{"begin":308,"end":309},"obj":"Gene"},{"id":"233","span":{"begin":85,"end":105},"obj":"Disease"}],"attributes":[{"id":"A232","pred":"tao:has_database_id","subj":"232","obj":"Gene:43740575"},{"id":"A233","pred":"tao:has_database_id","subj":"233","obj":"MESH:C000657245"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T196","span":{"begin":50,"end":52},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T197","span":{"begin":217,"end":234},"obj":"http://purl.obolibrary.org/obo/GO_0006277"},{"id":"T198","span":{"begin":284,"end":286},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T199","span":{"begin":358,"end":360},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}

{"project":"LitCovid-sentences","denotations":[{"id":"T250","span":{"begin":0,"end":106},"obj":"Sentence"},{"id":"T251","span":{"begin":107,"end":245},"obj":"Sentence"},{"id":"T252","span":{"begin":246,"end":457},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Here, we evaluated the use and suitability of the RT-LAMP assay for the detection of SARS-CoV-2 infection. We also developed LAMP-sequencing as a fully scalable alternative to colorimetric or fluorometric analysis of DNA amplification reactions. Our results indicate that whereas the RT-LAMP assay using the N-A primer set is not sensitive enough to replace RT-qPCR in all applications, it does hold promise as a method for testing large numbers of samples."}