PMC:7574920 / 32105-32589 JSONTXT

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T279","span":{"begin":275,"end":276},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"We noticed that the pH variability depended on the sample volume used for the RT-LAMP assay and the composition of the medium used for the swabs. For swabs in Amies medium (which was used for the clinical samples in this study), an RT-LAMP assay containing 1 μl of sample in a total volume of 20 μl was optimal. Our results obtained using native and heat-treated swab specimens suggested better performance when using heat treatment of swab specimens before running the RT-LAMP assay."}

{"project":"LitCovid-PubTator","denotations":[{"id":"228","span":{"begin":20,"end":22},"obj":"Gene"},{"id":"229","span":{"begin":159,"end":171},"obj":"Chemical"}],"attributes":[{"id":"A228","pred":"tao:has_database_id","subj":"228","obj":"Gene:5053"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"We noticed that the pH variability depended on the sample volume used for the RT-LAMP assay and the composition of the medium used for the swabs. For swabs in Amies medium (which was used for the clinical samples in this study), an RT-LAMP assay containing 1 μl of sample in a total volume of 20 μl was optimal. Our results obtained using native and heat-treated swab specimens suggested better performance when using heat treatment of swab specimens before running the RT-LAMP assay."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T193","span":{"begin":78,"end":80},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T194","span":{"begin":232,"end":234},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T195","span":{"begin":470,"end":472},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"We noticed that the pH variability depended on the sample volume used for the RT-LAMP assay and the composition of the medium used for the swabs. For swabs in Amies medium (which was used for the clinical samples in this study), an RT-LAMP assay containing 1 μl of sample in a total volume of 20 μl was optimal. Our results obtained using native and heat-treated swab specimens suggested better performance when using heat treatment of swab specimens before running the RT-LAMP assay."}

{"project":"LitCovid-sentences","denotations":[{"id":"T246","span":{"begin":0,"end":145},"obj":"Sentence"},{"id":"T247","span":{"begin":146,"end":311},"obj":"Sentence"},{"id":"T248","span":{"begin":312,"end":484},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"We noticed that the pH variability depended on the sample volume used for the RT-LAMP assay and the composition of the medium used for the swabs. For swabs in Amies medium (which was used for the clinical samples in this study), an RT-LAMP assay containing 1 μl of sample in a total volume of 20 μl was optimal. Our results obtained using native and heat-treated swab specimens suggested better performance when using heat treatment of swab specimens before running the RT-LAMP assay."}