| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T198 |
0-217 |
Sentence |
denotes |
On the basis of these preliminary experiments, we decided to use swab samples either directly without any treatment (direct swab–to–RT-LAMP assay) or after heat treatment for 5 min at 95°C (hot swab–to–RT-LAMP assay). |
| T199 |
218-329 |
Sentence |
denotes |
As an additional precaution, we kept the samples in the cold (using an ice-cold metal block) whenever possible. |
| T200 |
330-437 |
Sentence |
denotes |
For testing large numbers of clinical samples, we performed the RT-LAMP assay using several 96-well plates. |
| T201 |
438-596 |
Sentence |
denotes |
In total, we tested 209 different samples using the hot swab–to–RT-LAMP assay, and of these, 131 samples also were tested by the direct swab–to–RT-LAMP assay. |
| T202 |
597-744 |
Sentence |
denotes |
Many samples were tested twice but using aliquots withdrawn at different time points (usually within 24 hours) from the swab samples stored at 4°C. |
| T203 |
745-865 |
Sentence |
denotes |
This resulted in 235 direct swab–to–RT-LAMP assay measurements and 343 hot swab–to–RT-LAMP assay measurements (Fig. 5A). |
| T204 |
866-1108 |
Sentence |
denotes |
The hot swab–to–RT-LAMP assay detected a color change in the majority of samples with a CT < 30 with high sensitivity, whereas the direct swab–to–RT-LAMP assay only exhibited a high sensitivity for samples with a CT < 25 (Fig. 5 and Table 3). |
| T205 |
1109-1253 |
Sentence |
denotes |
The heat treatment rendered the RT-LAMP assay more stringent as it reduced false positives and more sensitive for samples with a CT of 25 to 30. |
| T206 |
1254-1358 |
Sentence |
denotes |
We found that some positive samples did not induce a color change but did so when assayed a second time. |
| T207 |
1359-1434 |
Sentence |
denotes |
We therefore would recommend running this assay using technical duplicates. |
