PMC:7574920 / 16724-17162 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T91","span":{"begin":144,"end":148},"obj":"Body_part"},{"id":"T92","span":{"begin":226,"end":230},"obj":"Body_part"}],"attributes":[{"id":"A91","pred":"fma_id","subj":"T91","obj":"http://purl.org/sig/ont/fma/fma74402"},{"id":"A92","pred":"fma_id","subj":"T92","obj":"http://purl.org/sig/ont/fma/fma74402"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T166","span":{"begin":31,"end":37},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T167","span":{"begin":45,"end":46},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T168","span":{"begin":82,"end":84},"obj":"http://purl.obolibrary.org/obo/CLO_0053733"},{"id":"T169","span":{"begin":144,"end":148},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T170","span":{"begin":171,"end":172},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T171","span":{"begin":226,"end":230},"obj":"http://purl.obolibrary.org/obo/OGG_0000000002"},{"id":"T172","span":{"begin":323,"end":324},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T52","span":{"begin":263,"end":265},"obj":"Chemical"}],"attributes":[{"id":"A52","pred":"chebi_id","subj":"T52","obj":"http://purl.obolibrary.org/obo/CHEBI_29388"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}

{"project":"LitCovid-PubTator","denotations":[{"id":"187","span":{"begin":75,"end":80},"obj":"Gene"},{"id":"188","span":{"begin":142,"end":143},"obj":"Gene"},{"id":"189","span":{"begin":321,"end":322},"obj":"Gene"}],"attributes":[{"id":"A187","pred":"tao:has_database_id","subj":"187","obj":"Gene:43740578"},{"id":"A188","pred":"tao:has_database_id","subj":"188","obj":"Gene:43740575"},{"id":"A189","pred":"tao:has_database_id","subj":"189","obj":"Gene:43740575"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T90","span":{"begin":8,"end":10},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T91","span":{"begin":231,"end":233},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T92","span":{"begin":344,"end":346},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T93","span":{"begin":390,"end":392},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}

{"project":"LitCovid-sentences","denotations":[{"id":"T106","span":{"begin":0,"end":267},"obj":"Sentence"},{"id":"T107","span":{"begin":268,"end":438},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"For the RT-LAMP assay, we also tested the 1a-A primer set directed against ORF1a (11) and found this primer set to be less sensitive than the N gene LAMP primer set, with a sensitivity cutoff of CT ≈ 25 when plotted against E gene RT-qPCR–derived CT values (fig. S3). On the basis of these results, we decided to use the N-A primer set for the RT-LAMP assay and to compare our results with RT-qPCR performed with the E-Sarbeco primer set."}