PMC:7574920 / 15171-15868 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T75","span":{"begin":71,"end":84},"obj":"Body_part"},{"id":"T76","span":{"begin":71,"end":74},"obj":"Body_part"},{"id":"T77","span":{"begin":315,"end":318},"obj":"Body_part"},{"id":"T78","span":{"begin":474,"end":487},"obj":"Body_part"},{"id":"T79","span":{"begin":474,"end":477},"obj":"Body_part"},{"id":"T80","span":{"begin":634,"end":637},"obj":"Body_part"},{"id":"T81","span":{"begin":641,"end":644},"obj":"Body_part"}],"attributes":[{"id":"A75","pred":"fma_id","subj":"T75","obj":"http://purl.org/sig/ont/fma/fma84126"},{"id":"A76","pred":"fma_id","subj":"T76","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A77","pred":"fma_id","subj":"T77","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A78","pred":"fma_id","subj":"T78","obj":"http://purl.org/sig/ont/fma/fma84126"},{"id":"A79","pred":"fma_id","subj":"T79","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A80","pred":"fma_id","subj":"T80","obj":"http://purl.org/sig/ont/fma/fma67095"},{"id":"A81","pred":"fma_id","subj":"T81","obj":"http://purl.org/sig/ont/fma/fma67095"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T149","span":{"begin":40,"end":41},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T150","span":{"begin":260,"end":261},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T151","span":{"begin":378,"end":379},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T152","span":{"begin":555,"end":556},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T50","span":{"begin":75,"end":84},"obj":"Chemical"},{"id":"T51","span":{"begin":478,"end":487},"obj":"Chemical"}],"attributes":[{"id":"A50","pred":"chebi_id","subj":"T50","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"},{"id":"A51","pred":"chebi_id","subj":"T51","obj":"http://purl.obolibrary.org/obo/CHEBI_25367"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}

{"project":"LitCovid-PubTator","denotations":[{"id":"173","span":{"begin":553,"end":554},"obj":"Gene"}],"attributes":[{"id":"A173","pred":"tao:has_database_id","subj":"173","obj":"Gene:43740575"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T80","span":{"begin":4,"end":6},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T81","span":{"begin":220,"end":222},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T82","span":{"begin":332,"end":334},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T83","span":{"begin":363,"end":365},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T84","span":{"begin":496,"end":498},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T85","span":{"begin":581,"end":583},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}

{"project":"LitCovid-sentences","denotations":[{"id":"T97","span":{"begin":0,"end":195},"obj":"Sentence"},{"id":"T98","span":{"begin":196,"end":279},"obj":"Sentence"},{"id":"T99","span":{"begin":280,"end":520},"obj":"Sentence"},{"id":"T100","span":{"begin":521,"end":697},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The RT-qPCR kit used was calibrated and a CT ≈ 30 corresponded to 1000 RNA molecules present in the reaction according to the certificate provided by the manufacturer (see Materials and Methods). The performance of each RT-qPCR run was validated using this as a positive control. Considering that 10 μl of isolated RNA was used for RT-qPCR, but only 1 μl for the RT-LAMP assay, a cutoff of CT ≈ 30 agreed well with the observed experimental sensitivity of approximately 100 RNA molecules for the RT-LAMP assay (Fig. 1A). Therefore, it appeared that the N-A primer set used for the RT-LAMP assay performed equally well with either IVT RNA or RNA samples isolated from the pharyngeal swab specimens."}