PMC:7574920 / 14326-15170 JSONTXT

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T57","span":{"begin":374,"end":376},"obj":"Disease"},{"id":"T58","span":{"begin":460,"end":462},"obj":"Disease"}],"attributes":[{"id":"A57","pred":"mondo_id","subj":"T57","obj":"http://purl.obolibrary.org/obo/MONDO_0017178"},{"id":"A58","pred":"mondo_id","subj":"T58","obj":"http://purl.obolibrary.org/obo/MONDO_0017178"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T136","span":{"begin":3,"end":4},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T137","span":{"begin":58,"end":59},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T138","span":{"begin":162,"end":163},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T139","span":{"begin":233,"end":235},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"},{"id":"T140","span":{"begin":261,"end":262},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T141","span":{"begin":411,"end":412},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T142","span":{"begin":557,"end":558},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T143","span":{"begin":574,"end":575},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T144","span":{"begin":611,"end":615},"obj":"http://purl.obolibrary.org/obo/UBERON_0000473"},{"id":"T145","span":{"begin":667,"end":669},"obj":"http://purl.obolibrary.org/obo/CLO_0001000"},{"id":"T146","span":{"begin":671,"end":672},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T147","span":{"begin":753,"end":754},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T148","span":{"begin":822,"end":823},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T48","span":{"begin":93,"end":99},"obj":"Chemical"},{"id":"T49","span":{"begin":104,"end":107},"obj":"Chemical"}],"attributes":[{"id":"A48","pred":"chebi_id","subj":"T48","obj":"http://purl.obolibrary.org/obo/CHEBI_15882"},{"id":"A49","pred":"chebi_id","subj":"T49","obj":"http://purl.obolibrary.org/obo/CHEBI_37958"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}

{"project":"LitCovid-PubTator","denotations":[{"id":"170","span":{"begin":536,"end":543},"obj":"Species"},{"id":"171","span":{"begin":93,"end":99},"obj":"Chemical"}],"attributes":[{"id":"A170","pred":"tao:has_database_id","subj":"170","obj":"Tax:9606"},{"id":"A171","pred":"tao:has_database_id","subj":"171","obj":"MESH:D019800"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T76","span":{"begin":18,"end":20},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T77","span":{"begin":603,"end":605},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T78","span":{"begin":791,"end":793},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T79","span":{"begin":836,"end":838},"obj":"http://purl.obolibrary.org/obo/GO_0001171"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}

{"project":"LitCovid-sentences","denotations":[{"id":"T92","span":{"begin":0,"end":148},"obj":"Sentence"},{"id":"T93","span":{"begin":149,"end":326},"obj":"Sentence"},{"id":"T94","span":{"begin":327,"end":445},"obj":"Sentence"},{"id":"T95","span":{"begin":446,"end":737},"obj":"Sentence"},{"id":"T96","span":{"begin":738,"end":844},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"In a colorimetric RT-LAMP reaction, positive samples with a CT \u003c 30 changed the color of the phenol-red dye within the first 30 min of the reaction. Samples with a CT \u003e 30 either did not change their color or did so at time points \u003e 35 min, simultaneously with a color change observed in some of the negative samples (Fig. 1). On the basis of this observation, we used the ΔOD value at 30 min to decide whether a sample was positive or negative. Plotting the ΔOD measurements versus CT values at the 30-min time point revealed that all patient samples with a CT \u003c 30 showed a robust color change in the RT-LAMP test, whereas for samples with CT values between 30 and 35, a positive result was observed for only 1 of 10 samples (Fig. 2C). This suggested a detection limit of the colorimetric RT-LAMP assay corresponding to a CT ≈ 30 for RT-qPCR."}