PMC:7572125 / 50884-51665 JSONTXT

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{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7572125","sourcedb":"PMC","sourceid":"7572125","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7572125","text":"Transfection efficiency was determined with a siGLO RISC-Free transfection control condition 48 hr after transfection by fluorescence microscopy, which was confirmed to be \u003e90% for all experiments reported. siRNA reagents: siGLO RISC-free (Dharmacon, cat# D-001600–01), siGenome non-targeting control 2 (Dharmacon, cat# D-001210–02), siGenome SERINC1 (Dharmacon, cat# M-010725–00), siGenome ZNF460 (Dharmacon, cat# M-032012–01), siGenome VAPA (Dharmacon, cat# M-021382–01), siGenome CNOT6L (Dharmacon, cat# M-016411–01), and siGenome PTEN (Dharmacon, cat# M-003023–02). 3’UTR plasmids: pcDNA-SERINC1-3’UTR, pcDNA-VAPA-3’UTR1, pcDNA-VAPA-3’UTR2, pcDNA-CNOT6L-3’UTR1, pcDNA-CNOT6L-3’UTR2, pCMV-MCS-PTEN-3’UTR (shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center).","tracks":[]}