PMC:7572125 / 44900-48159 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/7572125","sourcedb":"PMC","sourceid":"7572125","source_url":"https://www.ncbi.nlm.nih.gov/pmc/7572125","text":"Reagent type (species) or resource Designation Source or reference Identifiers Additional information\nCell line (Homo sapiens, male) DU145 ATCC cat#:HTB-81; RRID:CVCL_0105\nCell line (H. sapiens, male) Wild-type HCT116 cells Horizon Discovery cat# HD R02-019; RRID:CVCL_HD76\nCell line (H. sapiens, male) DICEREx5 HCT116 cells Horizon Discovery cat# HD R02-019; RRID:CVCL_HD76\nRecombinant DNA reagent psiCHECK-2+PTEN3’UTR Addgene plasmid# 50936; RRID:Addgene_50936\nRecombinant DNA reagent SERINC1 3’UTR doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nRecombinant DNA reagent VAPA 3’UTR1 doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nRecombinant DNA reagent VAPA 3’UTR2 doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nRecombinant DNA reagent CNOT6L 3’UTR1 doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nRecombinant DNA reagent CNOT6L 3’UTR2 doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nRecombinant DNA reagent PTEN 3’UTR doi:10.1016/j.cell.2011.09.029 Shared by Dr. Pier Paolo Pandolfi, Beth Israel Deaconess Medical Center\nSequence-based reagent siGlo RISC-free siRNA Dharmacon cat#:D-001600–01\nSequence-based reagent siGENOME non-targeting siRNA Dharmacon cat#:D-001210–02\nSequence-based reagent siGENOME SERINC1 Dharmacon cat# M-010725–00\nSequence-based reagent siGENOME ZNF460 Dharmacon cat# M-032012–01\nSequence-based reagent siGENOME VAPA Dharmacon cat# M-021382–01\nSequence-based reagent siGENOME CNOT6L Dharmacon cat# M-016411–01\nSequence-based reagent siGENOME PTEN Dharmacon M-003023–02\nSequence-based reagent TaqMan probe SERINC1 Thermo Fisher Scientific Hs00380375_m1\nSequence-based reagent TaqMan probe ZNF460 Thermo Fisher Scientific Hs01104252_m1\nSequence-based reagent TaqMan probe VAPA Thermo Fisher Scientific Hs00427749_m1\nSequence-based reagent TaqMan probe CNOT6L Thermo Fisher Scientific Hs00375913_m1\nSequence-based reagent TaqMan probe PTEN Thermo Fisher Scientific Hs02621230_s1\nSequence-based reagent TaqMan probe PARD3 Thermo Fisher Scientific Hs00969077_m1\nAntibody rabbit anti-PTEN Cell Signaling Technology cat#:9559; clone:138G5; RRID:AB_390810 1:1000 dilution\nAntibody mouse anti-HSP90 BD Biosciences cat#:610419; clone:68; RRID:AB_397798 1:1000 dilution\nAntibody HRP-conjugated donkey anti-rabbit GE Healthcare cat#:NA934; RRID:AB_772206 1:2000 dilution\nAntibody HRP-conjugated rabbit anti-mouse Abcam cat#:ab6728; RRID:AB_955440 1:2000 dilution\nSoftware, algorithm Veritas Microplate Luminometer software Turner BioSystems part#:998–9100; RRID:SCR_018534\nSoftware, algorithm StepOne Plus Real-Time PCR software Applied Biosystems RRID:SCR_014281 Version 2.3\nSoftware, algorithm ImageJ doi:10.1038/nmeth.2089 RRID:SCR_003070 Version 1.50a\nSoftware, algorithm Gen5 software BioTek Instruments RRID:SCR_017317 Version 2.05.5\nSoftware, algorithm R Project for statistical computing https://www.r-project.org RRID:SCR_001905 Version 3.5.1","divisions":[{"label":"tr","span":{"begin":0,"end":105}},{"label":"th","span":{"begin":0,"end":34}},{"label":"th","span":{"begin":36,"end":47}},{"label":"th","span":{"begin":49,"end":68}},{"label":"th","span":{"begin":70,"end":81}},{"label":"th","span":{"begin":83,"end":105}},{"label":"tr","span":{"begin":106,"end":178}},{"label":"td","span":{"begin":106,"end":136}},{"label":"td","span":{"begin":138,"end":143}},{"label":"td","span":{"begin":145,"end":149}},{"label":"td","span":{"begin":151,"end":178}},{"label":"tr","span":{"begin":179,"end":283}},{"label":"td","span":{"begin":179,"end":207}},{"label":"td","span":{"begin":209,"end":231}},{"label":"td","span":{"begin":233,"end":250}},{"label":"td","span":{"begin":252,"end":283}},{"label":"tr","span":{"begin":284,"end":387}},{"label":"td","span":{"begin":284,"end":312}},{"label":"td","span":{"begin":314,"end":335}},{"label":"td","span":{"begin":337,"end":354}},{"label":"td","span":{"begin":356,"end":387}},{"label":"tr","span":{"begin":388,"end":478}},{"label":"td","span":{"begin":388,"end":411}},{"label":"td","span":{"begin":413,"end":433}},{"label":"td","span":{"begin":435,"end":442}},{"label":"td","span":{"begin":444,"end":478}},{"label":"tr","span":{"begin":479,"end":623}},{"label":"td","span":{"begin":479,"end":502}},{"label":"td","span":{"begin":504,"end":517}},{"label":"td","span":{"begin":519,"end":549}},{"label":"td","span":{"begin":552,"end":623}},{"label":"tr","span":{"begin":624,"end":766}},{"label":"td","span":{"begin":624,"end":647}},{"label":"td","span":{"begin":649,"end":660}},{"label":"td","span":{"begin":662,"end":692}},{"label":"td","span":{"begin":695,"end":766}},{"label":"tr","span":{"begin":767,"end":909}},{"label":"td","span":{"begin":767,"end":790}},{"label":"td","span":{"begin":792,"end":803}},{"label":"td","span":{"begin":805,"end":835}},{"label":"td","span":{"begin":838,"end":909}},{"label":"tr","span":{"begin":910,"end":1054}},{"label":"td","span":{"begin":910,"end":933}},{"label":"td","span":{"begin":935,"end":948}},{"label":"td","span":{"begin":950,"end":980}},{"label":"td","span":{"begin":983,"end":1054}},{"label":"tr","span":{"begin":1055,"end":1199}},{"label":"td","span":{"begin":1055,"end":1078}},{"label":"td","span":{"begin":1080,"end":1093}},{"label":"td","span":{"begin":1095,"end":1125}},{"label":"td","span":{"begin":1128,"end":1199}},{"label":"tr","span":{"begin":1200,"end":1341}},{"label":"td","span":{"begin":1200,"end":1223}},{"label":"td","span":{"begin":1225,"end":1235}},{"label":"td","span":{"begin":1237,"end":1267}},{"label":"td","span":{"begin":1270,"end":1341}},{"label":"tr","span":{"begin":1342,"end":1416}},{"label":"td","span":{"begin":1342,"end":1364}},{"label":"td","span":{"begin":1366,"end":1387}},{"label":"td","span":{"begin":1389,"end":1398}},{"label":"td","span":{"begin":1400,"end":1416}},{"label":"tr","span":{"begin":1417,"end":1498}},{"label":"td","span":{"begin":1417,"end":1439}},{"label":"td","span":{"begin":1441,"end":1469}},{"label":"td","span":{"begin":1471,"end":1480}},{"label":"td","span":{"begin":1482,"end":1498}},{"label":"tr","span":{"begin":1499,"end":1568}},{"label":"td","span":{"begin":1499,"end":1521}},{"label":"td","span":{"begin":1523,"end":1539}},{"label":"td","span":{"begin":1541,"end":1550}},{"label":"td","span":{"begin":1552,"end":1568}},{"label":"tr","span":{"begin":1569,"end":1637}},{"label":"td","span":{"begin":1569,"end":1591}},{"label":"td","span":{"begin":1593,"end":1608}},{"label":"td","span":{"begin":1610,"end":1619}},{"label":"td","span":{"begin":1621,"end":1637}},{"label":"tr","span":{"begin":1638,"end":1704}},{"label":"td","span":{"begin":1638,"end":1660}},{"label":"td","span":{"begin":1662,"end":1675}},{"label":"td","span":{"begin":1677,"end":1686}},{"label":"td","span":{"begin":1688,"end":1704}},{"label":"tr","span":{"begin":1705,"end":1773}},{"label":"td","span":{"begin":1705,"end":1727}},{"label":"td","span":{"begin":1729,"end":1744}},{"label":"td","span":{"begin":1746,"end":1755}},{"label":"td","span":{"begin":1757,"end":1773}},{"label":"tr","span":{"begin":1774,"end":1835}},{"label":"td","span":{"begin":1774,"end":1796}},{"label":"td","span":{"begin":1798,"end":1811}},{"label":"td","span":{"begin":1813,"end":1822}},{"label":"td","span":{"begin":1824,"end":1835}},{"label":"tr","span":{"begin":1836,"end":1921}},{"label":"td","span":{"begin":1836,"end":1858}},{"label":"td","span":{"begin":1860,"end":1880}},{"label":"td","span":{"begin":1882,"end":1906}},{"label":"td","span":{"begin":1908,"end":1921}},{"label":"tr","span":{"begin":1922,"end":2006}},{"label":"td","span":{"begin":1922,"end":1944}},{"label":"td","span":{"begin":1946,"end":1965}},{"label":"td","span":{"begin":1967,"end":1991}},{"label":"td","span":{"begin":1993,"end":2006}},{"label":"tr","span":{"begin":2007,"end":2089}},{"label":"td","span":{"begin":2007,"end":2029}},{"label":"td","span":{"begin":2031,"end":2048}},{"label":"td","span":{"begin":2050,"end":2074}},{"label":"td","span":{"begin":2076,"end":2089}},{"label":"tr","span":{"begin":2090,"end":2174}},{"label":"td","span":{"begin":2090,"end":2112}},{"label":"td","span":{"begin":2114,"end":2133}},{"label":"td","span":{"begin":2135,"end":2159}},{"label":"td","span":{"begin":2161,"end":2174}},{"label":"tr","span":{"begin":2175,"end":2257}},{"label":"td","span":{"begin":2175,"end":2197}},{"label":"td","span":{"begin":2199,"end":2216}},{"label":"td","span":{"begin":2218,"end":2242}},{"label":"td","span":{"begin":2244,"end":2257}},{"label":"tr","span":{"begin":2258,"end":2341}},{"label":"td","span":{"begin":2258,"end":2280}},{"label":"td","span":{"begin":2282,"end":2300}},{"label":"td","span":{"begin":2302,"end":2326}},{"label":"td","span":{"begin":2328,"end":2341}},{"label":"tr","span":{"begin":2342,"end":2452}},{"label":"td","span":{"begin":2342,"end":2350}},{"label":"td","span":{"begin":2352,"end":2368}},{"label":"td","span":{"begin":2370,"end":2395}},{"label":"td","span":{"begin":2397,"end":2435}},{"label":"td","span":{"begin":2437,"end":2452}},{"label":"tr","span":{"begin":2453,"end":2551}},{"label":"td","span":{"begin":2453,"end":2461}},{"label":"td","span":{"begin":2463,"end":2479}},{"label":"td","span":{"begin":2481,"end":2495}},{"label":"td","span":{"begin":2497,"end":2534}},{"label":"td","span":{"begin":2536,"end":2551}},{"label":"tr","span":{"begin":2552,"end":2655}},{"label":"td","span":{"begin":2552,"end":2560}},{"label":"td","span":{"begin":2562,"end":2595}},{"label":"td","span":{"begin":2597,"end":2610}},{"label":"td","span":{"begin":2612,"end":2638}},{"label":"td","span":{"begin":2640,"end":2655}},{"label":"tr","span":{"begin":2656,"end":2751}},{"label":"td","span":{"begin":2656,"end":2664}},{"label":"td","span":{"begin":2666,"end":2698}},{"label":"td","span":{"begin":2700,"end":2705}},{"label":"td","span":{"begin":2707,"end":2734}},{"label":"td","span":{"begin":2736,"end":2751}},{"label":"tr","span":{"begin":2752,"end":2864}},{"label":"td","span":{"begin":2752,"end":2771}},{"label":"td","span":{"begin":2773,"end":2812}},{"label":"td","span":{"begin":2814,"end":2831}},{"label":"td","span":{"begin":2833,"end":2864}},{"label":"tr","span":{"begin":2865,"end":2971}},{"label":"td","span":{"begin":2865,"end":2884}},{"label":"td","span":{"begin":2886,"end":2921}},{"label":"td","span":{"begin":2923,"end":2941}},{"label":"td","span":{"begin":2943,"end":2958}},{"label":"td","span":{"begin":2960,"end":2971}},{"label":"tr","span":{"begin":2972,"end":3055}},{"label":"td","span":{"begin":2972,"end":2991}},{"label":"td","span":{"begin":2993,"end":2999}},{"label":"td","span":{"begin":3001,"end":3023}},{"label":"td","span":{"begin":3025,"end":3040}},{"label":"td","span":{"begin":3042,"end":3055}},{"label":"tr","span":{"begin":3056,"end":3143}},{"label":"td","span":{"begin":3056,"end":3075}},{"label":"td","span":{"begin":3077,"end":3090}},{"label":"td","span":{"begin":3092,"end":3110}},{"label":"td","span":{"begin":3112,"end":3127}},{"label":"td","span":{"begin":3129,"end":3143}},{"label":"td","span":{"begin":3144,"end":3163}},{"label":"td","span":{"begin":3165,"end":3200}},{"label":"td","span":{"begin":3202,"end":3227}},{"label":"td","span":{"begin":3229,"end":3244}}],"tracks":[]}